[Histonet] Re: Solvent Recycler
Miyamoto, Garret T Mr CIV USA USAMEDCOM
garret.t.miyamoto <@t> us.army.mil
Mon Sep 13 15:58:43 CDT 2010
Timothy,
We have the B/R 9700 ProCycler to recycle alcohol, xylene, and formalin. The Advantage must be a newer model. About 70 gal of alcohol, 40 gal of xylene, and 20 gal of formalin is recycled every 3 months. This recycler is easy to use and works well for us. As with any equipment, you need to maintain it by doing pm checks (replacing parts) as needed.
Garret
----- Original Message -----
From: histonet-request <@t> lists.utsouthwestern.edu
Date: Saturday, September 11, 2010 7:09 am
Subject: Histonet Digest, Vol 82, Issue 13
To: histonet <@t> lists.utsouthwestern.edu
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> Today's Topics:
>
> 1. Re: H&E+ Alcian Blue (Kim.Donadio <@t> bhcpns.org)
> 2. solvent recycling (Timothy Chilton)
> 3. Liver Processing Question (Herrick, James L. (Jim))
> 4. RE: solvent recycling (WILLIAM DESALVO)
> 5. RE: RE: Ventana vs. Leica (Makhijani, Nalini S)
> 6. RE: LIS, HIS question (Sharon Scalise)
> 7. Microm HM330 (Yu, Jian)
> 8. RE: LIS, HIS question (Feher, Stephen)
> 9. RE: solvent recycling (Feher, Stephen)
>
>
> -------------------------------------------------------------------
> ---
>
> Message: 1
> Date: Fri, 10 Sep 2010 12:46:11 -0500
> From: Kim.Donadio <@t> bhcpns.org
> Subject: Re: [Histonet] H&E+ Alcian Blue
> To: Deborah Faichney <
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> Content-Type: text/plain; charset="US-ASCII"
>
> It wont remain turquoise as long as you use the eosin. At least this is my
> experience. I have known some Pathologist to read the mucin that way
> (purple).
>
>
>
>
> Kim Donadio
> Pathology Supervisor
> Baptist Hospital
> 1000 W Moreno St.
> Pensacola FL 32501
> Phone (850) 469-7718
> Fax (850) 434-4996
>
>
>
> Deborah Faichney <
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 09/09/2010 03:44 AM
>
> To
> "histonet <@t> lists.utsouthwestern.edu" <
> cc
>
> Subject
> [Histonet] H&E+ Alcian Blue
>
>
>
>
>
>
> Hello all,
>
> I have a request to carry out a combined staining with H&E + Alcian Blue
> pH2.5. I have tried in vain to get this to work but regardless of the
> order of staining the end result is dark blue/purple mucin. I have
> carried out a parallel experiment whereby the staining has been checked
> microscopically then stopped after each of the dyes. (Thus giving 3
> slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really
> nicely stained but as soon as the eosin is added (using 2 different stains
> and a variety of times) the mucin staining looks similar to the nuclear
> stain. I am expecting the alcian blue to remain turquoise: should it?
>
> For information, I am trying to stain salmon intestine at 5 microns for
> using the following:
>
> Alcian Blue 8GX (certified), pH has been checked
> Haematoxylin Z (Cellpath Uk)
> 1% aq Eosin (Cellpath uk) and lab prepared solution from dye.
>
> Thanks from a frustrated technician!
>
> Debbie Faichney
> Histopathology
> Institute of Aquaculture
> University of Stirling
> Scotland
> UK
>
>
>
>
>
>
>
>
> --
> The Sunday Times Scottish University of the Year 2009/2010
> The University of Stirling is a charity registered in Scotland,
> number SC 011159.
>
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>
>
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> ------------------------------
>
> Message: 2
> Date: Fri, 10 Sep 2010 13:13:13 -0500
> From: "Timothy Chilton" <
> Subject: [Histonet] solvent recycling
> To: <
> Message-ID: <
> Content-Type: text/plain; charset=US-ASCII
>
> I am researching information on solvent recovery systems and could use some help specifically on B/R Instruments. We currently use the CBG Biotech recycler for alcohol use only but have been given funds from our environment of safety department to purchase two solvent recyclers to be used for alcohol, xylene, and formalin. If anyone is using the B/R 9700 ProCycler Advantage to regulate all three waste solvents in one system I would greatly appreciate any feedback.
>
> Thanks in advance,
>
> Timothy Chilton, HT (ASCP)
> Histology Supervisor
> University of Kansas Hospital
> 3901 Rainbow Blvd
> Kansas City, Kansas 66160
>
> phone: (913) 588-1134
> fax: (913) 588-8780
>
> tchilton <@t> kumc.edu
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 10 Sep 2010 14:33:58 -0500
> From: "Herrick, James L. (Jim)" <
> Subject: [Histonet] Liver Processing Question
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello everyone,
>
> I am trying to process and embed liver tissue in MMA. I used the same
> protocol that has worked very well a hundred times over, for harder
> tissues, but when used on the liver tissue, polymerized into a
> relatively soft and rubbery textured plastic for one of the two
> specimens (the second of the two also being too soft to properly
> section). If anyone would have any ideas/suggestions or protocols for me
> to follow, it would be very much appreciated. Thanks and have a great
> weekend!!
>
> Jim
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 10 Sep 2010 13:51:21 -0600
> From: WILLIAM DESALVO <
> Subject: RE: [Histonet] solvent recycling
> To: <, histonet <
> Message-ID: <
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> I suggest you look into Creative Waste Solutions Inc. for recycling (actually filtering through a slurry) your Formalin and Alcohols. I have over 8 years experience with the filtering systems. Dirty solution in, clean solution out and there is no breakdown of the chemical, just a cleaning (crystal clear and pure). For alcohol we combine several grades and then re-use them in all dilutions. Fresh alcohol is used for "100%" steps. For the Formalin, we combine filtered, test for concentration and buffering and add fresh 10% Formalin as needed to bring back into spec. Simple to use, very cost effective and the units are custom made to meet your volume of waste.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>
>
> > Date: Fri, 10 Sep 2010 13:13:13 -0500
> > From: tchilton <@t> kumc.edu
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] solvent recycling
> >
> > I am researching information on solvent recovery systems and could use some help specifically on B/R Instruments. We currently use the CBG Biotech recycler for alcohol use only but have been given funds from our environment of safety department to purchase two solvent recyclers to be used for alcohol, xylene, and formalin. If anyone is using the B/R 9700 ProCycler Advantage to regulate all three waste solvents in one system I would greatly appreciate any feedback.
> >
> > Thanks in advance,
> >
> > Timothy Chilton, HT (ASCP)
> > Histology Supervisor
> > University of Kansas Hospital
> > 3901 Rainbow Blvd
> > Kansas City, Kansas 66160
> >
> > phone: (913) 588-1134
> > fax: (913) 588-8780
> >
> > tchilton <@t> kumc.edu
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 10 Sep 2010 13:56:44 -0700
> From: "Makhijani, Nalini S" <
> Subject: RE: [Histonet] RE: Ventana vs. Leica
> To: "Mahoney,Janice A" <, "Nails, Felton"
> <, "Maria Katleba"
> <, "histonet"
> <
> Message-ID:
> <
> Content-Type: text/plain; charset="us-ascii"
>
> Jan,
> What a wonderful way to put everything in perspective! Definitely one
> of the most wise quotes by the timeless Beatles! I've been repeating the
> "Life is very short..." mantra all day. Thank you.
> On a similar vein is their "Let it be, let it be.." words.
> Any body else with Beatle words to live (sing) by?
> Nalini
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Mahoney,Janice A
> Sent: Wednesday, September 08, 2010 8:26 AM
> To: 'Nails, Felton'; 'Maria Katleba'; histonet
> Subject: [Histonet] RE: Ventana vs. Leica
>
> In My opinion (maybe we all need to start prefacing our comments with
> this), all of the Ventana reps I have encountered are very professional
> and would NEVER write something lilke this about a customer. I'm sure
> if it happened he would be fired on the spot.
> Perfect time for one of my favorite quotes of all time
> "Life is very short and there's no time for fussing and fighting my
> friends."
> -Lennon&Mccartney
> Jan Mahoney
> Omaha
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 10 Sep 2010 17:29:35 -0400
> From: "Sharon Scalise" <
> Subject: RE: [Histonet] LIS, HIS question
> To: <
> Message-ID: <
> Content-Type: text/plain; charset=US-ASCII
>
> We will be going live with Soft next March and I am looking for anyone currently using SoftPath to discuss some issues with. We are also interested in anyone using SoftPath that is doing immunohistochemistry. If you will be at the NSH meeting in Seattle we would love to meet with you and share information.
>
>
> Sharon E. Scalise, HTL (ASCP)
> Histology Supervisor
> William Beaumont Hospital
> Royal Oak, MI 48073
> 248 898-5981
> sscalise <@t> beaumonthospitals.com
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 10 Sep 2010 17:45:58 -0400
> From: "Yu, Jian" <
> Subject: [Histonet] Microm HM330
> To: "histonet <@t> lists.utsouthwestern.edu"
> <
> Message-ID:
> <
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I have a used HM330 which has been working well for past several years. Recently, the quick release clamp does not lock the knife/holder down well, which is causing problems in knife consumption and quality of sections. I paid ~$3000 about 4 years ago for this one. Does anyone know whether anyone services this and sell replacement parts, or I am better off get another used one. I have a small research lab with 3-4 people using the microtome for cutting mouse tissues, not on a daily basis.
>
> I am at University of Pittsburgh. Any info will be greatly appreciated.
>
> Thanks and have a great weekend,
>
> Jian Yu
> ****************************************************
> Jian Yu, Ph.D.
> Assistant Professor of Pathology
> University of Pittsburgh Cancer Institute
> Hillman Cancer Center Research Pavilion
> Office Suite 2.26h, Laboratory 2.43
> 5117 Centre Avenue, Pittsburgh, PA 15213
> �
> Phone: 412-623-7786, (Lab) 412-623-3255
> Fax:���412-623-7778
> Email: yuj2 <@t> upmc.edu
> ****************************************************
>
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 10 Sep 2010 19:24:59 -0400
> From: "Feher, Stephen" <
> Subject: RE: [Histonet] LIS, HIS question
> To: "Sharon Scalise" <,
> <
> Message-ID:
> <
> Content-Type: text/plain; charset="us-ascii"
>
> Sharon,
>
> We are using SoftPath with the Bond Max system. I will be presenting
> WS#50 Designing a LEAN Pathology Lab from Scratch, on Monday, Sept 27 at
> 8 am. We can meet after the workshop or perhaps it may come up during
> the workshop. I have quite a few lessons learned and would have, could
> have, should have's from my experience in bringing Soft in without
> having an LIS that it was replacing.
>
> I would be happy to meet with you to discuss this.
>
>
> Steve
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
> Scalise
> Sent: Friday, September 10, 2010 5:30 PM
> To: HISTONET <@t> PATHOLOGY.SWMED.EDU
> Subject: RE: [Histonet] LIS, HIS question
>
> We will be going live with Soft next March and I am looking for anyone
> currently using SoftPath to discuss some issues with. We are also
> interested in anyone using SoftPath that is doing immunohistochemistry.
> If you will be at the NSH meeting in Seattle we would love to meet with
> you and share information.
>
>
> Sharon E. Scalise, HTL (ASCP)
> Histology Supervisor
> William Beaumont Hospital
> Royal Oak, MI 48073
> 248 898-5981
> sscalise <@t> beaumonthospitals.com
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Fri, 10 Sep 2010 19:29:24 -0400
> From: "Feher, Stephen" <
> Subject: RE: [Histonet] solvent recycling
> To: "WILLIAM DESALVO" <, <,
> "histonet" <
> Message-ID:
> <
> Content-Type: text/plain; charset="us-ascii"
>
> I agree with William. We are currently using the Creative Waste
> Solutions recyclers pretty much the same way. One advantage is that
> Creative Waste will work with you using some pretty creative parameters
> to lease the recyclers. I suggest getting in touch with them. You will
> be surprised at the overall cost.
>
>
> Steve
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM
> DESALVO
> Sent: Friday, September 10, 2010 3:51 PM
> To: tchilton <@t> kumc.edu; histonet
> Subject: RE: [Histonet] solvent recycling
>
>
> I suggest you look into Creative Waste Solutions Inc. for recycling
> (actually filtering through a slurry) your Formalin and Alcohols. I have
> over 8 years experience with the filtering systems. Dirty solution in,
> clean solution out and there is no breakdown of the chemical, just a
> cleaning (crystal clear and pure). For alcohol we combine several grades
> and then re-use them in all dilutions. Fresh alcohol is used for "100%"
> steps. For the Formalin, we combine filtered, test for concentration and
> buffering and add fresh 10% Formalin as needed to bring back into spec.
> Simple to use, very cost effective and the units are custom made to meet
> your volume of waste.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>
>
> > Date: Fri, 10 Sep 2010 13:13:13 -0500
> > From: tchilton <@t> kumc.edu
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] solvent recycling
> >
> > I am researching information on solvent recovery systems and could use
> some help specifically on B/R Instruments. We currently use the CBG
> Biotech recycler for alcohol use only but have been given funds from our
> environment of safety department to purchase two solvent recyclers to be
> used for alcohol, xylene, and formalin. If anyone is using the B/R 9700
> ProCycler Advantage to regulate all three waste solvents in one system I
> would greatly appreciate any feedback.
> >
> > Thanks in advance,
> >
> > Timothy Chilton, HT (ASCP)
> > Histology Supervisor
> > University of Kansas Hospital
> > 3901 Rainbow Blvd
> > Kansas City, Kansas 66160
> >
> > phone: (913) 588-1134
> > fax: (913) 588-8780
> >
> > tchilton <@t> kumc.edu
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 82, Issue 13
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