[Histonet] Napsin A

[Jessica Piche]

Hi Loralee,
Thanks for your response. What did you use for the multi normal tissue block? 
Kidney?
Also for the multi-tumor block did you just use tumors from organs that  you 
knew were negative for Napsin or did you use both positive and negative tumors? 

Thanks, 
Jessica




________________________________
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
To: Jessica Piche <jhisto37 <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu" 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Mon, September 13, 2010 1:29:58 PM
Subject: RE: [Histonet] Napsin A

We are using a Lung Adenocarcinoma as a daily control.  Although I have noticed 
that normal lung macrophages will pick it up. 

We ran our validation of the antibody using a multi tumor block (contains 
various tumors from various organs) and a multi normal tissue block to ensure 
that it stained only the expected things and not anything else.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jessica Piche 
[jhisto37 <@t> yahoo.com]
Sent: Monday, September 13, 2010 11:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Napsin A

Hello Everyone,

We are in the process of working up Napsin A. I was wondering what tissue
others are using for multi tissue blocks to validate the anitbody? Thanks,


Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital, CT



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet