[Histonet] Flat Ice???

Mon Sep 13 09:52:41 CDT 2010

Thanks. I am going to try that "ice rink: tip. :-) 

What I do to keep my blocks from sliding all over the place now is put a 
kimwipe on my wet ice. They can cool and I also like to hydrate my blocks 
a little. I feel it gives me a better looking section histologically. my 2 
cents as well. ;o)

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

<histotech <@t> imagesbyhopper.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
09/11/2010 09:22 PM

To
"'WILLIAM DESALVO'" <wdesalvo.cac <@t> hotmail.com>, <msherwood <@t> partners.org>, 
<sraibley <@t> yahoo.com>, "'histonet'" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] Flat Ice???

I much prefer the "wet ice" approach to the more dry variety of the cool
trays.  The "wet" ice gets my blocks colder and I like the fact that it
introduces a small amount of moisture into the already faced block.  My
blocks are not on the ice for long periods of time, so over-saturating 
them
with water is not an issue.

To keep my trays from growing mountains, I have found that if I fill them 
up
3/4 of the way and let them freeze and then follow up with the other 1/4
later in the day, I get nice, flat little "ice rinks" that I can lay my
blocks on.  It's not time consuming either, just have to remember to go 
back
and pour more water on them!  By the way, this works on ice cube trays as
well as steel pans we have.  I don't really care for the pans, but some of
my techs do.  They take a little more work to make them ice rinks.  ;o)

My $0.02.  :o)

Michelle

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Friday, September 10, 2010 12:03 PM
To: msherwood <@t> partners.org; sraibley <@t> yahoo.com; histonet
Subject: RE: [Histonet] Flat Ice???

For quality assurance and safety concerns, I would suggest moving away 
from
the "wet ice" and go with a sealed freezing tray. We moved to the small
Histo-Cool ice trays w/ tray. 

 http://www.labstore.com/catalog/index.cfm?Category_ID=375

William DeSalvo, B.S., HTL(ASCP)

> Date: Fri, 10 Sep 2010 10:58:01 -0400
> From: MSHERWOOD <@t> PARTNERS.ORG
> To: sraibley <@t> yahoo.com; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Flat Ice???
> CC:
> 
> We use a styrofoam container and have no problem with it freezing 
> flat. Are you containers sitting flat in the freezer?
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Susan 
> Raibley
> Sent: Friday, September 10, 2010 10:52 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Flat Ice???
> 
> Hello! I would greatly appreciate any tips or tricks to getting ice to 
> freeze flat in the pan! We are tired of opening the freezer to see 
> mountains that have formed in our pans overnight, making it hard to 
> try and fit all the blocks we need to microtome on them. We have two 
> freezers and they both have this problem. Help please! Thanks!
> 
> 
> Susan Bincsik, HT (ASCP)
> 
> 
> 
> 
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