[Histonet] About home made mounting medium

alonso.martinezcanabal <@t> utoronto.ca alonso.martinezcanabal <@t> utoronto.ca
Wed Sep 8 09:21:19 CDT 2010


   Hi everyone.

     I am trying to make aquous mouning medium for our lab. I am doing  
10% PVV, 10% and 1% NPG in PBS. For a reason that I dont understand,  
when I try to mix the PVV wth the NPG the PVV starts to polymerize,  
even if it is completely dissolved. When I dissolve the NPG directly  
in the PVV solution it dissolves fine, but acquires a dark color that  
maybe be harmles but looks bad and apparently the appeareance is  
importnt in this lab... So, any suggestions?
Thank you very much

  Alonso




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