[Histonet] About home made mounting medium

[alonso.martinezcanabal <@t> utoronto.ca]

   Hi everyone.

     I am trying to make aquous mouning medium for our lab. I am doing  
10% PVV, 10% and 1% NPG in PBS. For a reason that I dont understand,  
when I try to mix the PVV wth the NPG the PVV starts to polymerize,  
even if it is completely dissolved. When I dissolve the NPG directly  
in the PVV solution it dissolves fine, but acquires a dark color that  
maybe be harmles but looks bad and apparently the appeareance is  
importnt in this lab... So, any suggestions?
Thank you very much

  Alonso