[Histonet] modified Mayers Hematoxylin

[Tony Henwood]

Margaret,

This might be of help:

Garvey's Modified Mayer's Haematoxylin

This solution is a progressive haematoxylin that uses 10% less alum. Chloral hydrate, because of its toxicity, has been replaced with ethanol, a more effective penetrator and bacteriostat. Mercury oxide has been replaced with sodium iodate (Garvie, (1991) J. Histotechn 14(3):164-165).

Fixation:      10% buffered formalin.

Microtomy:     paraffin sections at 5µm.

Reagents:
 
1.	Dissolve 45g ammonium or potassium alum in 900ml distilled water with the aid of heat. 
2.	Dissolve 2.5g Haematoxylin (CI 75290) in 100ml absolute ethanol.
3.	Combine above solutions and add 0.2g Sodium Iodate and 1g citric acid.
4.	Mix well. The solution is stable for several months.

Procedure:

1.	Dewax and hydrate sections.
2.	Stain in modified Mayer's Haematoxylin 40secs.
3.	Wash in warm water 5min.
4.	If required, counterstain in Eosin solution.
5.	Dehydrate, clear and mount.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Perry, Margaret
Sent: Friday, 3 September 2010 6:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] modified Mayers Hematoxylin


We are looking for a procedure for modified mayer's hematoxylin.  The pathologists  don't like the Gills.  We need something that is close to Mayer's in staining.  We were told to find something without the chloral hydrate.  We have found a modified Hematoxylin from Thermo that the doc's like but would like to keep costs down by making our own.    Can you help us?

Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

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