[Histonet] RE: accu-edge low profile microtome blades

[O'Donnell, Bill]

 The oil serves a valuable purpose and shopuld be found on all blades of
high quality. It protects against oxidation and allows the blades to be
easily separated from one another, so the oil has always been there. I
have personally found no advantage to wiping or not wiping the oil from
the slides. My 2cents - Bill

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sherwood, Margaret 
Sent: Wednesday, September 01, 2010 1:27 PM
To: Laurie Colbert; Brandi Higgins; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] accu-edge low profile microtome blades

We use the low-profile microtome blades and love them.  We actually
found Thermo-Fisher's brand of the low-profile to work just as well and
they are less expensive.  We had problems with other manufacturers' low
-profile blades that had the oil on them.  However we have several
sample boxes and I will have the techs wipe the oil off and see if they
work better.  Thanks, Laurie.

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Laurie
Colbert
Sent: Mon 8/30/2010 4:25 PM
To: Brandi Higgins; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] accu-edge low profile microtome blades



We also use those blades and love them.  We've tried others but always
come back to the Accu-edge.  I have always noticed a film of oil on the
blades.  I usually wipe the edge of the blade with a kimwipe before
using it.
Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Brandi
Higgins
Sent: Monday, August 30, 2010 1:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] accu-edge low profile microtome blades

Hello all,

We use the accu-edge low profile microtome blades (exclusively, as they
work best for us).  We noticed an oil/lubricant of some sort on the
blades in the last box that we opened (we checked one other box and it
has the same, although none previously did, or at least we didn't notice
it).  The oil is giving us problems with our sectioning.  Has anyone
else noticed this, either now or in the past?

Also, does anyone have a suggestions of other blades we should use?

Thanks for your input,
Brandi Higgins, BS, HT(ASCP)
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