[Histonet] breast fixation times

[Kuhnla, Melissa]

CAP/ASCO released guidelines this summer for ER and PR. Fixation should
be between 6 and  72 hours.  Time before fixation should be less than
one hour.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Wednesday, October 27, 2010 11:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] breast fixation times

There is no exception for core biopsies, as reasonable as that may seem.
I have had that discussion with the purveyors of the guidelines.  6-48
is the current standard.  there was a lot of discussion about exceeding
48 and using the FISH option.  My colleague responsible for this wrote:
It is in the CAP checklist, ANP 22998:

If the laboratory assesses Her2 by IHC or Her2 gene amplification by
in-situ hybridization (FISH, CISH, SISH), does the lab have a documented
procedure for ensuring appropriate length of fixation of specimens
tested? 

Specimens subject to Her2 testing should be fixed in 10% neutral
buffered formalin for at least 6 hours and no longer than 48 hours.
While fixation outside of these time limits is not an absolute exclusion
criterion for Her2 testing, labs should qualify any negative results for
specimens fixed less than 6 hours or longer than 48 hours. For cases
with negative results by IHC, consideration should be given to
performing confirmatory analysis by in-situ hybridization. 

 There is also a table in the original ASCO/CAP Guideline
Recommendations for Her2 in Breast Cancer (Arch Pathol Lab Med, Vol 131,
Jan 2007) that states that tissue fixed in formalin for greater than 48
hours is not an absolute exclusion criterion, but if known to be fixed
longer than 48 hours or unknown, the report should qualify any negative
result with this information (table 6). 

As for upcoming changes, i don't know other than these time limitations
are suppose to be more rigorously applied to ER and PR, along with the
newly instituted documentation of time between excision and time placed
in fixative.
 
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

[None] made the following annotations
---------------------------------------------------------------------
Confidential E-Mail: This e-mail is intended only for the person or
entity to which it is addressed, and may contain information that is
privileged, confidential, or otherwise protected from disclosure.
Dissemination, distribution, or copying of this e-mail or the
information herein by anyone other than the intended recipient is
prohibited. If you have received this e-mail in error, please notify the
sender by reply e-mail, and destroy the original message and all copies.

---------------------------------------------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail  and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you.