Fwd: [Histonet] FW: Saponin Technique

Brandi Higgins brandihiggins <@t> gmail.com
Mon Oct 25 11:08:58 CDT 2010

---------- Forwarded message ----------
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Date: Mon, Oct 25, 2010 at 12:08 PM
Subject: Re: [Histonet] FW: Saponin Technique
To: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>

We use Carnoy's to lyse the red blood cells.  We get thyroid and lymph node
FNA's regularly and we always process the slides through the Carnoy's.  The
solution we use is 120 ml 95% alcohol, 60 ml chloroform, and 20 ml glacial
acetic acid.  This 200 ml solution is good for the regular sized container
for a full rack of slides, but if you only have a few slides and not a whole
rack you can use 12 ml, 6 ml, 2ml (or any ratio you want obviously) so as
not to waste solution.  We place slides in Carnoy's for 5 minutes.  The
literature says 3-5 minutes, and not to exceed 15 minutes and you may
require more time in the hematoxlin following this procedure, although we
have not found that to be necessary.  Also please note that the solution
must be prepared fresh before use.  We already had glacal acetic acid on
hand when we started using Carnoy's so we just had to order chloroform.

Brandi Higgins, BS, H(ASCP)

On Mon, Oct 25, 2010 at 11:39 AM, Hannen, Valerie <
Valerie.Hannen <@t> parrishmed.com> wrote:

> ________________________________
> From: Hannen, Valerie
> Sent: Monday, October 25, 2010 11:39 AM
> To: 'histonet <@t> list.utsouthwestern.edu'
> Subject: Saponin Technique
> Hi folks..
> I am hoping someone out there in Histo-land can help. One of our
> Pathologists is asking us to check into doing the Saponin Technique to
> lyse the red cells in some of our cytology specimens. She gave a copy of
> one of the pages from one of her manuals, and it shows that in order for
> us to do this we would need to buy alot of reagents that we currently do
> not have. Being that we would probably not be doing this technique very
> often, and not sure how cost effective it will be for us to buy the
> reagents, we are wondering if  anyone knows of a commercial product that
> we can buy that would be a cheaper way out.
> Thanks in advance!!
> Valerie Hannen, Histotechnologist
> Parrish Medical Center
> Titusville,Florida
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