[Histonet] RE: Saponin Technique

[McMahon, Loralee A]

It's been a while but I think that we used to add a little diluted glacial acetic acid to our cytology specimens to lyse the red cells. 
Not sure on the dilution, but maybe someone else knows. 



Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [Valerie.Hannen <@t> parrishmed.com]
Sent: Monday, October 25, 2010 11:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: Saponin Technique

________________________________

From: Hannen, Valerie
Sent: Monday, October 25, 2010 11:39 AM
To: 'histonet <@t> list.utsouthwestern.edu'
Subject: Saponin Technique


Hi folks..
I am hoping someone out there in Histo-land can help. One of our
Pathologists is asking us to check into doing the Saponin Technique to
lyse the red cells in some of our cytology specimens. She gave a copy of
one of the pages from one of her manuals, and it shows that in order for
us to do this we would need to buy alot of reagents that we currently do
not have. Being that we would probably not be doing this technique very
often, and not sure how cost effective it will be for us to buy the
reagents, we are wondering if  anyone knows of a commercial product that
we can buy that would be a cheaper way out.

Thanks in advance!!
Valerie Hannen, Histotechnologist
Parrish Medical Center
Titusville,Florida


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