[Histonet] Re: Eosin to dye small Biopsies

[Patrick Laurie]

We have our grossing staff cover all GI biopsies, breast biopsies and
endometrial curettings or any small colorless tissue with 0.1% basic
fuchsin.  It leaves it a nice pinkish tint after processing, the specimens
are visible in the paraffin, and we haven't had any pathologist complaints.


On Fri, Oct 22, 2010 at 8:10 AM, <DKBoyd <@t> chs.net> wrote:

> As much as I respect Dr. Richmond, I would have to disagree that staining
> bx's with eosin is a waste of pathologist time.  It helps the embedding
> tech and cutting tech see the minute pieces, which may be otherwise lost.
> Sometimes that is the diagnostic material.
> We would not want to put a patient through another procedure because we
> couldn't recover the tissue submitted.
> We use a vial with a dropper.  Once the biopsy is placed in the cassette
> you take 1 second more to drop a drop of eosin on the specimen.
> Well worth everyone's time in my humble opinion.
>
>
> Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
> Center I
> 200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F:
> 804-765-5582 l dkboyd <@t> chs.net
>
>
>
>
>
>
>
> Robert Richmond <rsrichmond <@t> gmail.com>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 10/22/2010 10:44 AM
>
> To
> histonet <@t> lists.utsouthwestern.edu
> cc
>
> Subject
> [Histonet] Re: Eosin to dye small Biopsies
>
>
>
>
>
>
> Allison Scott at LBJ Hospital in Houston, Texas asks about the use of
> eosin to dye small biopsy specimens.
>
> Several replies mention addition of eosin to one of the processing
> alcohols. I have never seen this done, in maybe 60 pathology services
> I've worked in. (I'd know, because I nearly always examine the
> paraffin block when I order recuts or send a case out for
> consultation.)
>
> It's a fine time-waster for the pathologist to mark small specimens
> with dye while grossing. I've used Mercurochrome (merbromin, related
> to eosin but with 26% mercury) which fortunately was banned in the USA
> about ten years ago. I've used eosin, and I've used safranin (from the
> microbiology lab's Gram stain setup). I don't know whether safranin
> interferes with FISH, as eosin is well known to, nor do I know if you
> can put safranin in the processing alcohol. And I've used Davidson
> tissue marking inks.
>
> I've never seen or heard of cobalt blue used for this purpose - is
> this the insoluble coloring material, chemically cobalt aluminate?
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com