[Histonet] m.bovis

[Kathleen Jones]

Hello Histonet! 

I know this has come up in the past, but I have not been able to find a
solution in the archives...I am trying to get a mycoplasma bovis
protocol worked up and am having tremendous difficulties. My antibody
strain is M23 but it does not seem to be working very well for my IHC
applications. I have tried several antigen retrieval methods, antibody
dilutions and detection methods to no avail. I am thinking I need a new
source of antibody...Can anyone suggest a commercially available m.bovis
antibody that works for them? Any suggestions at all would be greatly
appreciated!

Thanks!!
Kathleen


Kathleen Jones
Research Technologist
Pathology/Microbiology
AVC - UPEI
(902)566-0595