[Histonet] Fwd: IHC coverglassing

[sgoebel <@t> xbiotech.com]

Absolutely!!  After the buffer rinse (after the counterstain), rinse in
water, then 95, then 100, then xylene, and coverslip away!!  I do it
everyday and it works fine =)


Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




-------- Original Message --------
Subject: [Histonet] Fwd: IHC coverglassing
From: Jen Campbell <jcampbell_mdl <@t> frontier.com>
Date: Tue, October 19, 2010 10:16 am
To: histonet <@t> lists.utsouthwestern.edu



Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

----- Forwarded Message -----
From: "Jen Campbell" <jcampbell_mdl <@t> frontier.com>
To: "histonet" <histonet-bounces <@t> lists.utsouthwestern.edu>
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can
"non-automated" IHC slides be dehydrated and cleared(xylene substitute)
after staining and be placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet