[Histonet] RE: Autofluorescence

Biedermann, JoAnn JABiedermann <@t> uams.edu
Mon Oct 18 14:04:58 CDT 2010


Richard, we had trouble with autofluorescence in brain tissue of the elderly. We have found that 1% Sudan Black B in 70% EtOH for 2 minutes before the secondary antibody works very well.

Jo Ann Biedermann
Research Assistant
University of Arkansas for Medical Sciences
Reynolds Institute on Aging
629 Jack Stephens Drive
Room 3173    Mail Slot 807
Little Rock, AR 72205
Phone: 501-526-5803
FAX: 501-526-5830
JABiedermann <@t> uams.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, October 18, 2010 12:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 83, Issue 29

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Today's Topics:

   1. RE: Re: Histonet Digest, Vol 83, Issue 20 (Weems, Joyce)
   2. RE: AFB (Weems, Joyce)
   3. RE: Re: Histonet Digest, Vol 83, Issue 20
      (histotech <@t> imagesbyhopper.com)
   4. Re: AFB (Lee & Peggy Wenk)
   5. autofluorescence (Edwards, Richard E.)
   6. RE: IHC FILD (Stephanie Rivera)
   7. (no subject) (Debora Probst)
   8. Eosin (sgoebel <@t> xbiotech.com)
   9. Block release (Bernice Frederick)
  10. RE: Block release (Helen Fedor)
  11. RE: Block release (Wanda.Smith <@t> HCAhealthcare.com)
  12. Re: Eosin (Rene J Buesa)
  13. clinical trial blocks (Tench, Bill)


----------------------------------------------------------------------

Message: 1
Date: Sun, 17 Oct 2010 18:46:54 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
To: "histotech <@t> imagesbyhopper.com" <histotech <@t> imagesbyhopper.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <92AD9B20A6C38C4587A9FEBE3A30E16403A6810A2F <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

I posted it when I found out about it last October. Sorry to everyone that didn't see it! j

-----Original Message-----
From: histotech <@t> imagesbyhopper.com [mailto:histotech <@t> imagesbyhopper.com]
Sent: Saturday, October 16, 2010 08:17
To: Weems, Joyce
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20

Joyce,

THANK YOU for the link!  :o)

Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update?

This is really going to help the "widget" count in my lab!  My paths frequently want to test more than one block on their tumor cases and now I can charge for them!

:o)

Michelle


-----Original Message-----
From: Weems, Joyce [mailto:JWeems <@t> sjha.org]
Sent: Friday, October 15, 2010 10:45 AM
To: histotech <@t> imagesbyhopper.com
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20


http://www.flpath.org/rli2.asp

Here is a link that will help..

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Friday, October 15, 2010 09:59
To: Weems, Joyce
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20

Can you please provide the specific CMS update number?  The website doesn't
seem to be too user friendly ...   thanks!



On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" <JWeems <@t> sjha.org> wrote:

>
> CMS/NCCI Update Dated October 1, 2009
>
> 8. The unit of service for special stains (CPT codes 88312-88313) and
> immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If
> it is medically reasonable and necessary to perform the same stain on
> more than one specimen or more than one block of tissue from the same
> specimen, additional units of service may be reported for the
> additional specimen(s) or block(s). Physicians should not report more
> than one unit of service for a stain performed on a single tissue
> block. For example it is common practice to cut multiple levels from a
> tissue block and stain each level with the same stain. The multiple
> levels from the same block of tissue stained with the same stain
> should not be reported as additional units of service. Only one unit
> of service should be reported for the stain on multiple levels from
> the single tissue block. Additionally, controls performed with special
> stains should not be reported as separate units of service for the
> stain.
>
>
> -----Original Message-----
> From: Mike Pence [mailto:mpence <@t> grhs.net]
> Sent: Thursday, October 14, 2010 11:31
> To: Weems, Joyce; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
>
> Can you site your source, please.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
> Joyce
> Sent: Thursday, October 14, 2010 10:25 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
>
>
>
>
> The change is that you can bill per block now and not per specimen.
> This is for immunos and special stains. It does make a huge difference!
>
> Best,
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chris
> Evanish
> Sent: Thursday, October 14, 2010 11:10
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
>
> Has anyone heard of a cpt coding change that allows us to bill 88342
> per slide run instead of per antibody? One of our Pathologist was at a
> conference and was told that we could do that. It makes a big
> difference with running cytokeratins on multiple blocks and levels of
> sentinel nodes.
>
>  Thanks,
> Chris Evanish
> Montgomery Hospital
> Norristown PA
>
> Chris D. Evanish
> Histology Supervisor
> Montgomery Hospital
> 610-270-2379
>
> Please consider the environment before printing this email " to your
> outgoing mail.
> Confidentiality Notice:
> This e-mail, including any attachments is the property of Catholic Health
East and is intended for the sole use of the intended recipient(s).
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>
>
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>
>
> _______________________________________________
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------------------------------

Message: 2
Date: Sun, 17 Oct 2010 18:47:41 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] AFB
To: Cheryl Crowder <ccrowder <@t> vetmed.lsu.edu>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <92AD9B20A6C38C4587A9FEBE3A30E16403A6810A30 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

I thought Fite's was more specific for leprosy.. whatever that bug is called now. j

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder
Sent: Saturday, October 16, 2010 10:49
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AFB

Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast bacteria.  The Fite's stain is usually considered specific for mycobacteria.
Cheryl

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------------------------------

Message: 3
Date: Sun, 17 Oct 2010 19:15:29 -0400
From: <histotech <@t> imagesbyhopper.com>
Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
To: "'Weems, Joyce'" <JWeems <@t> sjha.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <F75416DD10B6413E96EC8C85AC1A975C <@t> hopperPC>
Content-Type: text/plain; charset=US-ASCII

Joyce,

Certainly not *your* fault that *I* missed it!  THANK YOU for reposting the
link.  :o)

Michelle



-----Original Message-----
From: Weems, Joyce [mailto:JWeems <@t> sjha.org]
Sent: Sunday, October 17, 2010 6:47 PM
To: histotech <@t> imagesbyhopper.com
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20


I posted it when I found out about it last October. Sorry to everyone that
didn't see it! j

-----Original Message-----
From: histotech <@t> imagesbyhopper.com [mailto:histotech <@t> imagesbyhopper.com]
Sent: Saturday, October 16, 2010 08:17
To: Weems, Joyce
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20

Joyce,

THANK YOU for the link!  :o)

Now I wonder, if this is over a year old, is the the first time it's been
seen on Histonet, or did I just miss this important update?

This is really going to help the "widget" count in my lab!  My paths
frequently want to test more than one block on their tumor cases and now I
can charge for them!

:o)

Michelle


-----Original Message-----
From: Weems, Joyce [mailto:JWeems <@t> sjha.org]
Sent: Friday, October 15, 2010 10:45 AM
To: histotech <@t> imagesbyhopper.com
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20


http://www.flpath.org/rli2.asp

Here is a link that will help..

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Friday, October 15, 2010 09:59
To: Weems, Joyce
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20

Can you please provide the specific CMS update number?  The website doesn't
seem to be too user friendly ...   thanks!



On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" <JWeems <@t> sjha.org> wrote:

>
> CMS/NCCI Update Dated October 1, 2009
>
> 8. The unit of service for special stains (CPT codes 88312-88313) and
> immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If
> it is medically reasonable and necessary to perform the same stain on
> more than one specimen or more than one block of tissue from the same
> specimen, additional units of service may be reported for the
> additional specimen(s) or block(s). Physicians should not report more
> than one unit of service for a stain performed on a single tissue
> block. For example it is common practice to cut multiple levels from a
> tissue block and stain each level with the same stain. The multiple
> levels from the same block of tissue stained with the same stain
> should not be reported as additional units of service. Only one unit
> of service should be reported for the stain on multiple levels from
> the single tissue block. Additionally, controls performed with special
> stains should not be reported as separate units of service for the
> stain.
>
>
> -----Original Message-----
> From: Mike Pence [mailto:mpence <@t> grhs.net]
> Sent: Thursday, October 14, 2010 11:31
> To: Weems, Joyce; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
>
> Can you site your source, please.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
> Joyce
> Sent: Thursday, October 14, 2010 10:25 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
>
>
>
>
> The change is that you can bill per block now and not per specimen.
> This is for immunos and special stains. It does make a huge difference!
>
> Best,
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chris
> Evanish
> Sent: Thursday, October 14, 2010 11:10
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
>
> Has anyone heard of a cpt coding change that allows us to bill 88342
> per slide run instead of per antibody? One of our Pathologist was at a
> conference and was told that we could do that. It makes a big
> difference with running cytokeratins on multiple blocks and levels of
> sentinel nodes.
>
>  Thanks,
> Chris Evanish
> Montgomery Hospital
> Norristown PA
>
> Chris D. Evanish
> Histology Supervisor
> Montgomery Hospital
> 610-270-2379
>
> Please consider the environment before printing this email " to your
> outgoing mail.
> Confidentiality Notice:
> This e-mail, including any attachments is the property of Catholic Health
East and is intended for the sole use of the intended recipient(s).
> It may contain information that is privileged and confidential.  Any
unauthorized review, use, disclosure, or distribution is prohibited. If you
are not the intended recipient, please delete this message, and reply to the
sender regarding the error in a separate email.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice:
> This e-mail, including any attachments is the property of Catholic
> Health East and is intended for the sole use of the intended
> recipient(s). It may contain information that is privileged and
> confidential.  Any unauthorized review, use, disclosure, or
> distribution is prohibited. If you are not the intended recipient,
> please delete this message, and reply to the sender regarding the
> error in a separate email.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

_______________________________________________
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Confidentiality Notice:
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for the sole use of the intended recipient(s).
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not the intended recipient, please delete this message, and
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No virus found in this incoming message.
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Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10
18:34:00

Confidentiality Notice:
This e-mail, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
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not the intended recipient, please delete this message, and
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No virus found in this incoming message.
Checked by AVG - www.avg.com
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18:33:00




------------------------------

Message: 4
Date: Sun, 17 Oct 2010 22:02:23 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] AFB
To: "Cheryl Crowder" <ccrowder <@t> vetmed.lsu.edu>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <474B6E602A164B3C90484AFE22963783 <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
        reply-type=original

Hi - Can't find the original question - must have deleted it, so I'll answer
through Cheryl's, if that's OK with her.

If I remember, the question was something like - are there other
microorganisms besides TB that stain with Kinyoun (or something like that).

First, any mycobacterium (acid fast bacteria AFB) will stain with
Ziehl-Neelsen, Kinyoun, Auramine-Rhodamine or Fites (hence anything staining
with these stains is called AFB positive, meaning staining with these AF
stains) . So that means Mycobacterium tuberculosis (TB), Mycobacterium avium
intracellular (found in birds and I've seen it in spleen and bone marrow of
immune suppressed patients), Mycobacterium paratuberculosis (found in
intestine of infected cows and goats, causing something similar to Crone's
disease), and a lot of other Mycobacterium are AFB positive staining with
these procedures.

Mycobacterium leprae (leprosy) is a very thin walled mycobacterium, so needs
the Fites stain with the peanut oil (or any other oil, such as mineral oil)
to coat the leprosy microorganism, so it can withstand decolorization with
aqueous alcohol. But Fites can also be used to demonstrate any of the
Mycobacterium.

These AFB stains will also demonstrate the sulfur clubs of actinomyces, and
the filamentous strands of nocardia are also weakly AFB positive. (Sort of
bacteria with some fungus characteristics.)

Cryptosporidium found in the intestine (causing severe diarrhea) can also
stain AFB positive.

And there are bacterium (mycobacterium or others) in tap water that are AFB
positive, which can adhere to your slides from the flotation bath water, or
from the tap water.  Usually non-pathogenic AFB, but I don't know their
names.

And I'm sure there are other less common bacteria that I've forgotten or
never known about that will stain AFB positive, which are not in the
Mycobacterium genus.

And let's remember that hair follicles, sperm (heads with lipids),
lipofucsin (wear and tear pigment of lipids) also stain AFB positive. And
also Russell bodies (inclusions of antibodies in plasma cells) will stain
AFB positive. And keratin which is dense can simply require longer
differentiation, or else it will retain the red color of the dye.

So, yes, Amy, there are other things that stain positive with Kinyoun that
are not TB/mycobacterium.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

--------------------------------------------------
From: "Cheryl Crowder" <ccrowder <@t> vetmed.lsu.edu>
Sent: Saturday, October 16, 2010 10:48 AM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] AFB

> Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast
> bacteria.  The Fite's stain is usually considered specific for
> mycobacteria.
> Cheryl
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 5
Date: Mon, 18 Oct 2010 10:51:36 +0100
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] autofluorescence
To: "Histonet <@t> lists.utsouthwestern.edu"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <7722595275A4DD4FA225B92CDBF174A1E8DB32C9DF <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"

Any information on current methods to  counter  autoflourescence in formalin fixed paraffin processed tissues would be much appreciated.

                                   Many thanks

                                    Richard  Edwards

                                            Leicester  University
                                                   U.K................





------------------------------

Message: 6
Date: Mon, 18 Oct 2010 08:13:07 -0500
From: Stephanie Rivera <stephanie.d.rivera <@t> gsk.com>
Subject: [Histonet] RE: IHC FILD
To: Debora Probst <Debora.Probst <@t> crhs.net>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <14A77A757E86BC499A34E86B47649B64054FC6BA7D <@t> 019D-NAMSG-05.019D.MGD.MSFT.NET>

Content-Type: text/plain; charset="us-ascii"

My company does not recognize the certification. It was just for my personal development. Some hospitals do recognize it as a specialty but I don't know if you get an increase. Check with your manager.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Debora Probst
Sent: Friday, October 15, 2010 1:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC FILD

Can anyone tell me if once you have taken the IHC certification test and
passed dose the administration consider that a specialty field and give
you a pay increase? Or is it just for a persons own gratification to
take it and pass?

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 7
Date: Mon, 18 Oct 2010 10:51:08 -0400
From: "Debora Probst" <Debora.Probst <@t> crhs.net>
Subject: [Histonet] (no subject)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <4843CFFC8DC9A54E88E4AB3F5A50689819C1A93F <@t> crhs_exch.nterprise.crhs.net>

Content-Type: text/plain;       charset="us-ascii"

I would just like to thank everyone for their response back on IHC
certification. What a great resource for our field (Histology). Again
thank you everyone.

Debora Probst

Columbus Regional, Columbus Ga.



------------------------------

Message: 8
Date: Mon, 18 Oct 2010 07:53:00 -0700
From: <sgoebel <@t> xbiotech.com>
Subject: [Histonet] Eosin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <20101018075300.9e2d9aa830e8449a2412eb1e4f2f067e.1df96a717f.wbe <@t> email04.secureserver.net>

Content-Type: text/plain; charset="utf-8"


   Once  upon a time I heard that eosin fluoresces is this true?    color  does it show up?  Could this be used as a sort of back gr   stain so you can tell exactly how many cells are in the field?
   Sarah Goebel, B.A., HT (ASCP)
   Histotechnician
      8201 East Riversid   Austin, Texas   (512)386-2907


------------------------------

Message: 9
Date: Mon, 18 Oct 2010 10:04:23 -0500
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: [Histonet] Block release
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0AD641BB647144BCBC1AE4CD68C18B22 <@t> lurie.northwestern.edu>
Content-Type: text/plain;       charset="US-ASCII"

Everyone,

I have been asked to post a query as to what your institution does in terms
of releasing blocks for oncology clinical trials. Please respond as it is
important to us as we receive a lot of those blocks here at Northwestern
(policies, procedures, alternative submissions etc) If you are in Australia,
Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of
know already) as we do get blocks from you also.

Thanks

Bernice





Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

ECOGPCO-RL

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723





------------------------------

Message: 10
Date: Mon, 18 Oct 2010 11:12:08 -0400
From: Helen Fedor <hfedor <@t> jhmi.edu>
Subject: RE: [Histonet] Block release
To: Bernice Frederick <b-frederick <@t> northwestern.edu>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <3201CF51728F6048A24FA3AFFFEEF1D31781F6FE65 <@t> JHEMTEXVS3.win.ad.jhu.edu>
Content-Type: text/plain; charset="us-ascii"

Hello, Our institution does release blocks, the requirement is that a patient consent is signed and we have an MTA (Material Transfer Agreement) For the study and appropriate IRB's in place for the study. It is a lot to set up, but after this is done for one study any following studies are easier to handle.

Helen

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice Frederick
Sent: Monday, October 18, 2010 11:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Block release

Everyone,

I have been asked to post a query as to what your institution does in terms
of releasing blocks for oncology clinical trials. Please respond as it is
important to us as we receive a lot of those blocks here at Northwestern
(policies, procedures, alternative submissions etc) If you are in Australia,
Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of
know already) as we do get blocks from you also.

Thanks

Bernice





Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

ECOGPCO-RL

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 11
Date: Mon, 18 Oct 2010 10:22:33 -0500
From: <Wanda.Smith <@t> HCAhealthcare.com>
Subject: RE: [Histonet] Block release
To: <b-frederick <@t> northwestern.edu>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9E2D36CE2D7CBA4A94D9B22E8328A3BA1395E57654 <@t> NADCWPMSGCMS03.hca.corpad.net>

Content-Type: text/plain; charset="us-ascii"

Good Morning to All,
We, as often as possible, try not to release blocks for studies or legal cases.  Most studies will let you submit 10-20 unstained recut slides on Plus slides.  On legal cases, we always give recuts if there is enough tissue.  When slides will not suffice, we will send the block and keep a record of the block being sent.
Wanda


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice Frederick
Sent: Monday, October 18, 2010 11:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Block release

Everyone,

I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also.

Thanks

Bernice





Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

ECOGPCO-RL

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 12
Date: Mon, 18 Oct 2010 08:51:43 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Eosin
To: histonet <@t> lists.utsouthwestern.edu, sgoebel <@t> xbiotech.com
Message-ID: <9500.31952.qm <@t> web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if you observe a solution with transmitted light.
Ren? J.

--- On Mon, 10/18/10, sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com> wrote:


From: sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com>
Subject: [Histonet] Eosin
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, October 18, 2010, 10:53 AM



???Once? upon a time I heard that eosin fluoresces is this true? = ; What
???color? does it show up?? Could this be used as a sort of back gr= ound
???stain so you can tell exactly how many cells are in the field?
???Sarah Goebel, B.A., HT (ASCP)
???Histotechnician
???= = XBiotech USA Inc.
???8201 East Riversid= e Dr. Bldg 4 Suite 100
???Austin, Texas=???78744
???(512)386-2907
_______________________________________________
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------------------------------

Message: 13
Date: Mon, 18 Oct 2010 08:58:44 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] clinical trial blocks
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56FE <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii

The fundamental philosophy that we have is that we do not want to stand
in the way of the patient receiving any kind of treatment.  That being
said, we also have the policy that whether or not we "own" the material
(patients are actually suppose to sign an admission consent that says
they give up any ownership rights), we are clearly the legal custodians
of the material, and as such are responsible for insuring that no
irreparable distruction occurs.  So, we prefer to not send out blocks
unless the clilnical trial says there are no alternatives.  We contact
them and ask if we can send recuts from which they can obtain the
information they desire.  We also review what we have.  If we have only
one section, we cut a recut for the file before sending out any blocks.
If we have only one block, with not much in it, we again discuss the
issue with the trial coordinator.  We do insist on return of the block,
and assuming that there is an outside review of the material, we insist
on a copy of the report.  We also have the patient sign a release
informing them that they need to understand that this process may
exhaust any tissue that would be useful for some other new treatment
which may come up down the line. Since we have to pull this material for
review and usually make recuts, we bill the clinical trial.  Years back,
they never had any budget allowances for this, and it was always a
hassle.  More recently, we get a lot less BS.  They know they have to
pay, and they do.  Unfortunately, nothing is free any more.

Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604


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