[Histonet] negative controls

Sebree Linda A LSebree <@t> uwhealth.org
Fri Oct 15 11:07:59 CDT 2010 

To clarify even further:  we cut 2 patient sections, put one on a slide with a section of positive control already on it.  This slide gets stained with the antibody  The other section goes on another slide and is run as a corresponding negative control using the same antibody protocol but substituting a negative control serum for the antibody, thus this is a "negative reagent control" slide.  Elements within the patient slide that received antibody and are expected to be negative, serve as a "negative tissue control".  Again, we run 1 negative control slide for EVERY 1 patient block in a run but only 1 negative control per any number of antibodies run on that same block, using the harshest protocol.  Only autopsy cases differ in that we run 1 negative control per TISSUE TYPE.


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: sgoebel <@t> xbiotech.com [mailto:sgoebel <@t> xbiotech.com] 
Sent: Friday, October 15, 2010 10:48 AM
To: Rene J Buesa
Cc: Histo Net list server; Sebree Linda A
Subject: RE: [Histonet] negative controls

So for every HP you do, you process a control cassette with the patient
tissue cassette?  That seems like alot?  How do you get that many
control tissues on a daily basis?  What do you do with the remaining
tissue in the control block?  If you throw them away everyday, I would
be interested in some of them.  How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time?  We have always just had a bunch of
blocks that you cut a control from?  I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue.  If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times.  Why couldn't you
just have one for all 3 cases?  Then the next day have a fresh ONE for
that day, date them, and file them.  So if you needed to see the HP
control for October 15th, you could go pull the control for that day...

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-5107




-------- Original Message --------
Subject: RE: [Histonet] negative controls
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A <LSebree <@t> uwhealth.org>, sgoebel <@t> xbiotech.com
Cc: Histo Net list server <HistoNet <@t> lists.utsouthwestern.edu>

Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J. 

--- On Fri, 10/15/10, sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com> wrote:


From: sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com>
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Cc: "Histo Net list server" <HistoNet <@t> lists.utsouthwestern.edu>
Date: Friday, October 15, 2010, 11:17 AM

 
   Why do you need a negative control for each block if you are runn=
ing
   the  same  antibody  on each patient block?  Is it just for case by c
  ase  reference  so  the negative is filed with the patient slide?  Why
   co=  uldn't  you  have  a control slide bank that was dated so all
the
   slides you d= id on that day, on that run, could be referenced back
to
   that control? = ; Just curious?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician<= br>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bld= g 4 Suite 100

   Austin, Texas  78744

   =

   (512)386-= 5107

   -------- Original Message --------
   Subject: RE: [Histonet] negative controls
   From: "Sebree Linda A" <[1]LSebree@= uwhealth.org>
   Date: Fri, October 15, 2010 8:08 am
   To:  "Victoria  Baker"  <[2]bakevict= oria <@t> gmail.com>, "Histo Net
list
   server"
   <[3]HistoNet <@t> lists.uts= outhwestern.edu>
   We run negative controls on every block of a case within the same
run.
   On autopsy cases, we only run 1 negative per tissue type, within the
   same run...this is the only exception to the rule of 1 negative per
   block.
   Linda A. Sebree
   University of Wisconsin Hospital & Clinics
   IHC/ISH Laboratory
   DB1-223 VAH
   600 Highland Ave.
   Madison, WI 53792
   (608)265-6596
   -----Original Message-----
   From: [4]histonet= -bounces <@t> lists.utsouthwestern.edu
   [[5]mailto:histon=  et-bounces <@t> lists.utsouthwestern.edu]  On Behalf
Of
   Victoria
   Baker
   Sent: Friday, October 15, 2010 9:26 AM
   To: Histo Net list server
   Subject: [Histonet] negative controls
   Hi
   I have a hypothetical question to those who run IHC on Ventana
   instruments.
   Are you running your negatives with your patient/test cases or on a
   separate
   run? Also, if you are doing this and have to use a different
detection
   kit
   how do you work the QA/QC portion of this for CAP requirements.
   Thanks
   Vikki
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References

   1. 3D"mailto:LSebree <@t> uwhealth.org"
   2. 3D"mailto:bakevictoria <@t> gmail.com"
   3. 3D"mailto:HistoNet <@t> lists.utsouthwestern.edu"
   4. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
   5. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
   6. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
   7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
   8. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
   9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
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