[Histonet] Re: Histonet Digest, Vol 83, Issue 20
Mike Pence
mpence <@t> grhs.net
Thu Oct 14 15:57:16 CDT 2010
Now for someone to do the same study using FISH!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chris
Evanish
Sent: Thursday, October 14, 2010 10:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
Ibarra JA and Rogers LW: Fixation time does not affect expression of
HER2/neu. Am J Clin Pathol 2010;134:594-596.
If you leave the tissue in the 70% for 36 hours, doesn't it cause the
tissue to become extra hardened do to the alcohol?
Chris D. Evanish
Histology Supervisor
Montgomery Hospital
610-270-2379
Please consider the environment before printing this email " to your
outgoing mail.
>>> <histonet-request <@t> lists.utsouthwestern.edu> 10/14/2010 9:04 AM >>>
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."
Today's Topics:
1. pathology reports (Horn, Hazel V)
2. Seeking Grossing/Histo Tech (Mighnon Lashus)
3. Re: Urinary bladder diverticula (Robert Richmond)
4. pathology reports (Tench, Bill)
5. Re: Histonet Digest, Vol 83, Issue 19 (Cathy.Crumpton <@t> tuality.org)
6. RE: RE: New Cap Guidelines for Her2 and ER/PR (Kuhnla, Melissa)
7. RE: RE: New Cap Guidelines for Her2 and ER/PR (Bill B.)
8. RE: RE: New Cap Guidelines for Her2 and ER/PR (McMahon, Loralee A)
9. FW: How to remove Hematoxilin (Margaryan, Naira)
10. I agree with Jose, etal. (Orr, Rebecca)
11. Training labs in the San Francisco Bay Area? (Morken, Tim)
12. RE: New Cap Guidelines for Her2 and ER/PR (Amos Brooks)
13. Re: Training labs in the San Francisco Bay Area?
(Jennifer MacDonald)
14. Re: IHC OOps wrong secondary (Kimberly Tuttle)
15. "2010 Focus on IHC" one day event comes to NJ (Pedro Louro)
16. Advanced workshop in 3D live cell imaging in Sydney on 16-19
November. (Anya Salih)
17. fridge/freezer storage space (Edwards, Richard E.)
18. RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR (Patsy Ruegg)
19. RE: fridge/freezer storage space (John Shelley)
----------------------------------------------------------------------
Message: 1
Date: Wed, 13 Oct 2010 12:06:50 -0500
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: [Histonet] pathology reports
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<25A4DE08332B19499904459F00AAACB7181249D05B <@t> EVS1.archildrens.org>
Content-Type: text/plain; charset="ISO-8859-1"
When you have results from an outside lab, i.e. flow results, bone
marrows, ect. How do you integrate this into your path report?
It is a verbatim copy inside the report in the same format as the
outside lab? Or can it be a copy with the results in a different
format? A discussion has occurred within in or transcription
department over this matter. We do attach the outside report to the
hard copy report in our paper file.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
******************************************************
The information contained in this message may be privileged and
confidential and protected from disclosure. If the reader of this
message is not the
intended recipient, or an employee or agent responsible for delivering
this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please
notify
us immediately by replying to the message and deleting it from your
computer. Thank you.
------------------------------
Message: 2
Date: Wed, 13 Oct 2010 12:21:05 -0500
From: Mighnon Lashus <MLashus <@t> pathgroup.com>
Subject: [Histonet] Seeking Grossing/Histo Tech
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Cc: Dewayne Belew <dbelew <@t> pathgroup.com>
Message-ID:
<197CD0B02A81F94994A285C59C8AE05C05F49D4CDB <@t> pgnexchange.pathgroup.com>
Content-Type: text/plain; charset="us-ascii"
We are seeking a qualified candidate for the position of Grossing/Lead
Histo Tech in our Chattanooga location. We are a state of art
laboratory using the Ventana Vantage system to maintain specimen
integrity; we also have Ventana special stainers and the Benchmark Ultra
and XTs for our Immunohistochemistry stains. We offer a friendly
working environment along with an excellent benefit package. You may
obtain more information about this position at CareerBuilders.com. We
also have an opening for a cytotechnologist at our Pathology office on
the Erlanger Health System campus in Chattanooga, TN.
Mighnon Lashus, HT (ASCP)
PathGroup Lab
4071 S. Access Road, Suite 107
Chattanooga, TN 37406
423-493-0207
423-493-0208 fax
mlashus <@t> pathgroup.com
________________________________
Important Notice: This e-mail is intended for the use of the person to
whom it is addressed and may contain information that is privileged and
confidential. If you are not the intended recipient, any disclosure,
copying, distribution, or use of the contents of this message is
strictly prohibited. If you have received this e-mail in error, please
destroy this message and contact the Security Officer at PathGroup, Inc
immediately at 615-562-9255. Thank you
------------------------------
Message: 3
Date: Wed, 13 Oct 2010 13:26:33 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Urinary bladder diverticula
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTik_8nzHuf6FpxMyh9VEfJ7UimXLfW0mpGmzWpz8 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Joyce Weems in Atlanta asks:
>>If TUR is 88307 and resection is 88309, what CPT code do you use for
>>bladder diverticuli?<<
A transurethral resection of the prostate (TURP) is coded 88305 no
matter how many blocks. A TUR of the bladder (presumably a TURBT, that
is, a TUR of a bladder tumor) is 88307. A cystectomy specimen for cancer
(resection) is 88309.
There are no specific instructions for a diverticulum of the urinary
bladder. Diverticula of the GI tract are however coded 88305, and I
would code a urinary tract diverticulum (bladder or urethra) as 88305
also.
The singular is diverticulum, the plural is diverticula. There is no
such word as *diverticuli.
If you don't have a copy of the anatomic pathology CPT codes, 2010
edition, I can send you a PDF of a scan of those pages.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 4
Date: Wed, 13 Oct 2010 10:30:24 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] pathology reports
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A56C2 <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii
We scan all outside laboratory reports (including consultations, flow
cytometry, special immunohistochemistry, molecular tests, etc) and
insert the scanned material into an addendum which is electronically
tied to the primary report. If the outside report has graphic material
(ie, material that cannot be converted into a "WORD" format) it has to
be excised because at least our vision of Cerner Millenium will not
permit non-text material to be inserted into the report document (We use
an Epson scanner and Epson program that does a very handy job of
converting PDF documents into WORD documents and allows for the excision
of non-WORD material---like fancy headers). When appropriate, ie, it is
not obvious what the results mean, we may add our own comment about how
these results should be interpreted in the setting of our other
material. This saves a lot of transcription (which use to be the way we
accomplished this) and avoids transcriptional errors. It generally
allows capture of the name and address of the outside laboratory, which
is a CLIA requirement. Incorporating the results of special tests into
the report is a CAP requirement.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
[None] made the following annotations
---------------------------------------------------------------------
Confidential E-Mail: This e-mail is intended only for the person or
entity to which it is addressed, and may contain information that is
privileged, confidential, or otherwise protected from disclosure.
Dissemination, distribution, or copying of this e-mail or the
information herein by anyone other than the intended recipient is
prohibited. If you have received this e-mail in error, please notify the
sender by reply e-mail, and destroy the original message and all copies.
---------------------------------------------------------------------
------------------------------
Message: 5
Date: Wed, 13 Oct 2010 10:40:39 -0700
From: Cathy.Crumpton <@t> tuality.org
Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 19
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF4DC0EC3C.CA2AE1F2-ON882577BB.00611B22-882577BB.00611B4F <@t> tuality.org>
Content-Type: text/plain; charset="ISO-8859-1"
We are not open on weekends and worked out a deal with our core l
that is open 24/7. When we have a breast on the processer we will
run a Sat. program that ends at 21:00. They added a task on their
dai They j were being embed the embedding cent embedding.
No harm
Cathy Tuality Community Hospital
Hillsbo (503)681-1292
------------------------------
Message: 6
Date: Wed, 13 Oct 2010 13:51:45 -0400
From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
To: Mike Pence <mpence <@t> grhs.net>, Phyllis Thaxton <dchihc <@t> yahoo.com>,
"Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>, Joyce Cline
<Joyce.Cline <@t> wchsys.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C76F12086768614F9C2618ED9966FEE10698F36B <@t> MMDCNT0BXVS003.chsli.org>
Content-Type: text/plain; charset="US-ASCII"
Fixation prior to the processor will not exce4ed 12 hrs. That is why we
program the processor for 36...not to exceed 48.
________________________________
From: Mike Pence [mailto:mpence <@t> grhs.net]
Sent: Wednesday, October 13, 2010 11:04 AM
To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
How much time is the tissue in formalin prior to going in the processor?
Your total time in formalin can not exceed 48 hr. And you will still
need to validate your process if you hold your tissue longer than
"normal processing" time in 70% (ie. more than 1 hr).
-----Original Message-----
From: Kuhnla, Melissa [mailto:Melissa.Kuhnla <@t> chsli.org]
Sent: Wednesday, October 13, 2010 9:45 AM
To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
For the weekend, we have our processor set for 36 hours in
formalin and then a hold in 70%. This allows for complete fixation and
cuts down on prolonged time in 70%
________________________________
From: Phyllis Thaxton [mailto:dchihc <@t> yahoo.com]
Sent: Wednesday, October 13, 2010 10:32 AM
To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
We run a weekend (Friday til Monday AM) breast run where the
tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in
order to complete processing on Monday morning. So far no problems.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
________________________________
From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence
<mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>;
histonet <@t> lists.utsouthwestern.edu
Sent: Tue, October 12, 2010 12:02:32 PM
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
I disagree. Prolonged formalin fixation (over 48 hrs),
diminishes
signals
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 12:05 PM
To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
Formalin fixation time does not impact the results of FISH as it
does
IHC.
Jan M
Omaha
-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net]
Sent: Tuesday, October 12, 2010 11:00 AM
To: Mahoney,Janice A; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
I don't think it matters if you do Her2 by FISH or IHC the time
is still
48hr. I hope I am wrong, but I don't think I am.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 10:25 AM
To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
We have decided to reflex to FISH those breasts that do not fall
within
the recommended formalin fixation time. We do work on Saturdays
so it
is only the rare 3 day weekends that this comes into play. Jan M
Omaha
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Joyce
Cline
Sent: Tuesday, October 12, 2010 10:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR
Does anyone have any experience with storing formalin fixed
breast
tissue in 70% before processing? I am trying to comply with the
new
guidelines set forth by CAP and ASCO with regard to Her2 and
ER/PR and
since my lab does not operate on the weekend we have been well
above the
48 hour recommended formalin fixation time. Does 70% affect
antigenicity for either Her2 or ER/PR? Any information or
suggestions
will be greatly appreciated. Thanks :)
Ronda Souders
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>
________________________________
***** CONFIDENTIALITY NOTICE ***** This message contains
confidential
information and is intended only for the individual named. If
you are
not the named addressee you should not disseminate, distribute
or copy
this e-mail. Please notify the sender immediately by e-mail if
you have
received this e-mail by mistake and delete this e-mail from your
system.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Sponsored by Catholic Health Initiatives and Immanuel, Alegent
Health is
faithful to the healing ministry of Jesus Christ, providing high
quality
care for the body, mind and spirit of every person.
The information contained in this communication, including
attachments,
is confidential and private and intended only for the use of the
addressees. Unauthorized use, disclosure, distribution or
copying is
strictly prohibited and may be unlawful. If you received this
communication in error, please inform us of the erroneous
delivery by
return e-mail message from your computer. Additionally,
although all
attachments have been scanned at the source for viruses, the
recipient
should check any attachments for the presence of viruses before
opening.
Alegent Health accepts no liability for any damage caused by any
virus
transmitted by this e-mail. Thank you for your cooperation.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Sponsored by Catholic Health Initiatives and Immanuel, Alegent
Health is
faithful to the healing ministry of Jesus Christ, providing high
quality
care for the body, mind and spirit of every person.
The information contained in this communication, including
attachments,
is confidential and private and intended only for the use of the
addressees. Unauthorized use, disclosure, distribution or
copying is
strictly prohibited and may be unlawful. If you received this
communication in error, please inform us of the erroneous
delivery by
return e-mail message from your computer. Additionally,
although all
attachments have been scanned at the source for viruses, the
recipient
should check any attachments for the presence of viruses before
opening.
Alegent Health accepts no liability for any damage caused by any
virus
transmitted by this e-mail. Thank you for your cooperation.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The information in this e-mail, and any attachments therein, is
confidential and for use by the intended addressee only. If this message
is received by you in error please do not disseminate or read further.
Please reply to the sender that you have received the message in error,
then delete the message. Although Catholic Health Services of Long
Island attempts to sweep e-mail and attachments for viruses, it does
not guarantee that either are virus-free and accepts no liability for
any damage sustained as a result of viruses. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The information in this e-mail, and any attachments therein, is
confidential and for use by the intended addressee only. If this message
is received by you in error please do not disseminate or read further.
Please reply to the sender that you have received the message in error,
then delete the message. Although Catholic Health Services of Long
Island attempts to sweep e-mail and attachments for viruses, it does not
guarantee that either are virus-free and accepts no liability for any
damage sustained as a result of viruses. Thank you.
The information in this e-mail, and any attachments therein, is
confidential and for use by the intended addressee only. If this message
is received by you in error please do not disseminate or read further.
Please reply to the sender that you have received the message in error,
then delete the message. Although Catholic Health Services of Long
Island attempts to sweep e-mail and attachments for viruses, it does
not guarantee that either are virus-free and accepts no liability for
any damage sustained as a result of viruses. Thank you.
------------------------------
Message: 7
Date: Wed, 13 Oct 2010 14:12:59 -0500
From: "Bill B." <bill501 <@t> mindspring.com>
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <p06240800c8dbb47898bf@[4.245.12.30]>
Content-Type: text/plain; charset="us-ascii"
How do you define fixation time? A half pound hunk of fat with a tumor
in the middle will remain unfixed until blocked. A small biopsy will
start fixing almost immediately.
Bill
At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote:
>Fixation prior to the processor will not exce4ed 12 hrs. That is why
>we program the processor for 36...not to exceed 48.
>
>
>
>________________________________
>
>From: Mike Pence [mailto:mpence <@t> grhs.net]
>Sent: Wednesday, October 13, 2010 11:04 AM
>To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
>
>
>How much time is the tissue in formalin prior to going in the
>processor? Your total time in formalin can not exceed 48 hr. And you
>will still need to validate your process if you hold your tissue longer
>than "normal processing" time in 70% (ie. more than 1 hr).
>
> -----Original Message-----
> From: Kuhnla, Melissa [mailto:Melissa.Kuhnla <@t> chsli.org]
> Sent: Wednesday, October 13, 2010 9:45 AM
> To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
>
> For the weekend, we have our processor set for 36 hours in
formalin
>and then a hold in 70%. This allows for complete fixation and cuts down
>on prolonged time in 70%
>
>
>
>
>________________________________
>
>
> From: Phyllis Thaxton [mailto:dchihc <@t> yahoo.com]
> Sent: Wednesday, October 13, 2010 10:32 AM
> To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
>
>
>
>
> We run a weekend (Friday til Monday AM) breast run where the
tissues
>are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order
>to complete processing on Monday morning. So far no problems.
>
>
>
> Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL
>
>
>
>
>
>
>________________________________
>
>
> From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
> To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence
><mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>;
>histonet <@t> lists.utsouthwestern.edu
> Sent: Tue, October 12, 2010 12:02:32 PM
> Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
>
> I disagree. Prolonged formalin fixation (over 48 hrs),
diminishes
> signals
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Mahoney,Janice A
> Sent: Tuesday, October 12, 2010 12:05 PM
> To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
>
> Formalin fixation time does not impact the results of FISH as it
does
> IHC.
> Jan M
> Omaha
>
> -----Original Message-----
> From: Mike Pence [mailto:mpence <@t> grhs.net]
> Sent: Tuesday, October 12, 2010 11:00 AM
> To: Mahoney,Janice A; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>
> I don't think it matters if you do Her2 by FISH or IHC the time
is
>still
> 48hr. I hope I am wrong, but I don't think I am.
>
> Mike
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Mahoney,Janice A
> Sent: Tuesday, October 12, 2010 10:25 AM
> To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
>
> We have decided to reflex to FISH those breasts that do not fall
>within
> the recommended formalin fixation time. We do work on Saturdays
so it
> is only the rare 3 day weekends that this comes into play. Jan M
>Omaha
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Joyce
> Cline
> Sent: Tuesday, October 12, 2010 10:10 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR
>
> Does anyone have any experience with storing formalin fixed
breast
> tissue in 70% before processing? I am trying to comply with the
>new
> guidelines set forth by CAP and ASCO with regard to Her2 and
>ER/PR and
> since my lab does not operate on the weekend we have been well
>above the
> 48 hour recommended formalin fixation time. Does 70% affect
> antigenicity for either Her2 or ER/PR? Any information or
>suggestions
> will be greatly appreciated. Thanks :)
>
>
> Ronda Souders
> Hagerstown Medical Laboratory
> 301-665-4980
> fax 301-665-4941
> ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>
------------------------------
Message: 8
Date: Wed, 13 Oct 2010 15:23:10 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
To: "Bill B." <bill501 <@t> mindspring.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C27AA2A01CEF31469813089E226F582E0393BC6F27 <@t> URMCMS7.urmc-sh.rochester.ed
u>
Content-Type: text/plain; charset="us-ascii"
If our cases are needle cores they are almost immediately put into
formalin from the patient. That time is recorded by the nurse or the
clinician that took the specimen.
The larger breast samples are received fresh from the OR and are
immediately grossed either by a PA or resident. Those samples are
examined, sliced (to expose the surface area of the specimen) and placed
into formalin. That is when the fixation time is recorded for the
larger specimens.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bill B.
[bill501 <@t> mindspring.com]
Sent: Wednesday, October 13, 2010 3:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
How do you define fixation time? A half pound hunk of fat with a tumor
in the middle will remain unfixed until blocked. A small biopsy will
start fixing almost immediately.
Bill
At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote:
>Fixation prior to the processor will not exce4ed 12 hrs. That is why
>we program the processor for 36...not to exceed 48.
>
>
>
>________________________________
>
>From: Mike Pence [mailto:mpence <@t> grhs.net]
>Sent: Wednesday, October 13, 2010 11:04 AM
>To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
>
>
>How much time is the tissue in formalin prior to going in the
>processor? Your total time in formalin can not exceed 48 hr. And you
>will still need to validate your process if you hold your tissue longer
>than "normal processing" time in 70% (ie. more than 1 hr).
>
> -----Original Message-----
> From: Kuhnla, Melissa [mailto:Melissa.Kuhnla <@t> chsli.org]
> Sent: Wednesday, October 13, 2010 9:45 AM
> To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>
> For the weekend, we have our processor set for 36 hours in
>formalin and then a hold in 70%. This allows for complete fixation and
>cuts down on prolonged time in 70%
>
>
>
>
>________________________________
>
>
> From: Phyllis Thaxton [mailto:dchihc <@t> yahoo.com]
> Sent: Wednesday, October 13, 2010 10:32 AM
> To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>
>
>
>
> We run a weekend (Friday til Monday AM) breast run where the
>tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in
>order to complete processing on Monday morning. So far no problems.
>
>
>
> Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL
>
>
>
>
>
>
>________________________________
>
>
> From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
> To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>; Mike Pence
><mpence <@t> grhs.net>; Joyce Cline <Joyce.Cline <@t> wchsys.org>;
>histonet <@t> lists.utsouthwestern.edu
> Sent: Tue, October 12, 2010 12:02:32 PM
> Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>
> I disagree. Prolonged formalin fixation (over 48 hrs),
>diminishes
> signals
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Mahoney,Janice A
> Sent: Tuesday, October 12, 2010 12:05 PM
> To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>
> Formalin fixation time does not impact the results of FISH as it
>does
> IHC.
> Jan M
> Omaha
>
> -----Original Message-----
> From: Mike Pence [mailto:mpence <@t> grhs.net]
> Sent: Tuesday, October 12, 2010 11:00 AM
> To: Mahoney,Janice A; Joyce Cline;
>histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and
>ER/PR
>
> I don't think it matters if you do Her2 by FISH or IHC the time
>is still
> 48hr. I hope I am wrong, but I don't think I am.
>
> Mike
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Mahoney,Janice A
> Sent: Tuesday, October 12, 2010 10:25 AM
> To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
>
>
> We have decided to reflex to FISH those breasts that do not fall
>within
> the recommended formalin fixation time. We do work on Saturdays
>so it
> is only the rare 3 day weekends that this comes into play. Jan M
>Omaha
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>Joyce
> Cline
> Sent: Tuesday, October 12, 2010 10:10 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR
>
> Does anyone have any experience with storing formalin fixed
>breast
> tissue in 70% before processing? I am trying to comply with the
>new
> guidelines set forth by CAP and ASCO with regard to Her2 and
>ER/PR and
> since my lab does not operate on the weekend we have been well
>above the
> 48 hour recommended formalin fixation time. Does 70% affect
> antigenicity for either Her2 or ER/PR? Any information or
>suggestions
> will be greatly appreciated. Thanks :)
>
>
> Ronda Souders
> Hagerstown Medical Laboratory
> 301-665-4980
> fax 301-665-4941
> ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Wed, 13 Oct 2010 16:03:28 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] FW: How to remove Hematoxilin
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1BA93040C6B9A4A8D84878F93FEC36A039D2050DA <@t> CMHEXCC01MBX.childrensmemori
al.org>
Content-Type: text/plain; charset="us-ascii"
Hi histoworld,
I would like to repeat my staining on the slides already coverslipped
but need to remove hematoxilin first. How to remove hematoxilin? Is it
need to repeat Antigen retrieval?
Thanks in advance,
Naira
------------------------------
Message: 10
Date: Wed, 13 Oct 2010 16:29:49 -0500
From: "Orr, Rebecca" <ROrr <@t> northshore.org>
Subject: [Histonet] I agree with Jose, etal.
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B44CA3426A2C084F9BA90B93D528159B04D2C9488A <@t> EXCH34.enhnet.org>
Content-Type: text/plain; charset="us-ascii"
Thanks for your response, Jose, I agree with your points.
I see these guidelines as the first attempt to standardize something
that has numerous variables. How can we expect to have the
manufacturers of these markers offer (or be required by the FDA)
consistent results when our end of this process is "all over the board"?
An ER, PR Her2 that we both buy from the same manufacturer should work
the exact same way in my lab as it does in yours, IF we both process in
the exact same way. I doubt very much if histology and IHC testing will
ever be as exact as a clinical chemistry assay for example, but it's a
start. I forsee the time gap in formalin shortening as labs get used to
this first step. This would mean more validation, but since we should be
doing validation each year, then this may not be such a giant task.
Becky Orr CLA,HT(ASCP)QIHC
Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem
847-570-2771
------------------------------
Message: 11
Date: Wed, 13 Oct 2010 15:12:54 -0700
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] Training labs in the San Francisco Bay Area?
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1AAF670737F193429070841C6B2ADD4C026967E880 <@t> EXMBMCB15.ucsfmedicalcenter.
org>
Content-Type: text/plain; charset=us-ascii
To anyone in the San Francisco Bay area, generally northern half: Is
anyone interested in training a neophyte for histotechnology?
A person in San Francisco contacted me about learning histology. He came
and observed in our lab and is very interested but needs a lab to train
in. He is looking at online histology programs in the meantime. He does
not have a degree in biology/chemistry (it is in literature) but he did
work summers in a lab during high school and college and actually did
limited histology at that time.
If you can help him out contact me directly. No need to post back to
Histonet.
Thanks!
Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA
415.514.6042 (office)
415.885.7409 Fax
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
------------------------------
Message: 12
Date: Wed, 13 Oct 2010 18:21:22 -0400
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
To: JEllin <@t> yumaregional.org, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTimUkwqHFLPC1YYdZ+66ihAJxuQyhDC+fOTkGPJz <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
I'm going to have to disagree with this approach. Lumping all
specimens into the same procedure (fixation/processing time) is in no
way a step toward individualized care that is so often discussed. Doing
this ignores the basic fundamental differences between the specimens. A
liver is different from a breast and a brain. Likewise a particularly
fatty breast is different from a fiberous one, or one that is cut
smaller than the one the other pathologist (or PA) stuffed into a
cassette. Each of these situations needs to be addressed differently
from fixation to processing times and to be entirely honest staining
times in many cases. I fear you may be oversimplifying the situation and
calling it standardization.
All the best,
Amos
Date: Wed, 13 Oct 2010 08:05:27 -0700
From: "Jesus Ellin" <JEllin <@t> yumaregional.org>
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
To: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>, "Phyllis
Thaxton"
<dchihc <@t> yahoo.com>, "Mahoney,Janice A" <
Janice.Mahoney <@t> alegent.org>,
"Mike Pence" <mpence <@t> grhs.net>, "Joyce Cline"
<Joyce.Cline <@t> wchsys.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<29BE166A2CF48D459853F8EC57CD37E8021C68C9 <@t> EXCHANGECLUSTER.yumaregional.l
ocal
>
Content-Type: text/plain; charset="US-ASCII"
OK I usually do not like to chime in on this, but here I go. How can a
true validation of a specific target be obtained if the wiggle room is 6
to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the
results for this testing if you are going to be varying process for the
weekend vs weekday? This is the flaw in the guidelines in my
perspective, when this much time is allowed it is like anything else. We
are going to go the path of least resistance to change, instead of what
is right. I know that as techs we always want the best, but are pushed
to produce next day. Techs for years have been saying more fixation is
needed on tissue. Well enough of that.
What we do is we hold at 12 hours of fixation for all specimens no
matter what? We document ischemic cold time through our LIS, to include
time placed in formalin, and time of first cut. We feel that all
specimens need the same fixation times. This is imperative to
standardize the process, but once again we also have our processors set
up in such a way that they come off at different times and our
production of H an E is in sync with this. It might sound like a lot,
but we get most of our work done around 96 to 97 % of cases within 24
hours or less using conventional processing techniques.
With the future relying more and more on, patient centered care, through
personalized medicine, we need to really look on how we can do the
optimal requirements, not do the minimal requirements to reach our
goals.
Jesus Ellin
------------------------------
Message: 13
Date: Wed, 13 Oct 2010 15:30:21 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Training labs in the San Francisco Bay Area?
To: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF3E5472D1.158A0BFC-ON882577BB.007B009D-882577BB.007BA428 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
With the on-line programs the training facility will need to be approved
by the program. They will need to sign an affiliation agreement between
the program (school) and the training site.
"Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/13/2010 03:17 PM
To
Histonet <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Training labs in the San Francisco Bay Area?
To anyone in the San Francisco Bay area, generally northern half: Is
anyone interested in training a neophyte for histotechnology?
A person in San Francisco contacted me about learning histology. He came
and observed in our lab and is very interested but needs a lab to train
in. He is looking at online histology programs in the meantime. He does
not have a degree in biology/chemistry (it is in literature) but he did
work summers in a lab during high school and college and actually did
limited histology at that time.
If you can help him out contact me directly. No need to post back to
Histonet.
Thanks!
Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA
415.514.6042 (office)
415.885.7409 Fax
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 13 Oct 2010 19:14:57 -0400
From: "Kimberly Tuttle" <ktuttle <@t> umm.edu>
Subject: Re: [Histonet] IHC OOps wrong secondary
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Kimberly Tuttle"
<ktuttle <@t> umm.edu>
Message-ID: <4CB60536.90CE.001A.3 <@t> umm.edu>
Content-Type: text/plain; charset=US-ASCII
Thanks to everyone who responded. I removed the coverslip, and ran it
back to water and re applied the secondary, dab and counterstain. It
worked like a charm.
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax
Please consider the environment before printing this e-mail.
>>> "Kimberly Tuttle" <ktuttle <@t> umm.edu> 10/13/2010 12:07 pm >>>
Is it possible for me to remove the coverslip, run back to water and
re-use the slides starting with the correct secondary?
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax
Please consider the environment before printing this e-mail.
This e-mail and any accompanying attachments may be privileged,
confidential, contain protected health information about an identified
patient or be otherwise protected from disclosure. State and federal law
protect the confidentiality of this information. If the reader of this
message is not the intended recipient; you are prohibited from using,
disclosing, reproducing or distributing this information; you should
immediately notify the sender by telephone or e-mail and delete this
e-mail.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This e-mail and any accompanying attachments may be privileged,
confidential, contain protected health information about an identified
patient or be otherwise protected from disclosure. State and federal law
protect the confidentiality of this information. If the reader of this
message is not the intended recipient; you are prohibited from using,
disclosing, reproducing or distributing this information; you should
immediately notify the sender by telephone or e-mail and delete this
e-mail.
------------------------------
Message: 15
Date: Wed, 13 Oct 2010 20:34:17 -0400
From: Pedro Louro <histologyinfo <@t> gmail.com>
Subject: [Histonet] "2010 Focus on IHC" one day event comes to NJ
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTikMYxjZ2PAEe9VApB2T+Fono4OPK8ETWaOhTHrO <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
The date is set, the topics are in place, and the money is just right,
all we need....is you to attend.
When: Friday, November 5, 2010
Where: Somerset Medical Center
110 Rehill Ave
Somerville, NJ 08876
*"2010 Focus on IHC"* presented by NJSH and sponsored by BioCare
Medical, LLC
$10.00(members) $40.00 (Non-members) gets you:
- 6
CEU's
-
Breakfast, Lunch and Snacks
-
Great learning experience
Hope to see you there,
Pedro Louro
President (New Jersey Society for Histotechnology)
Co-chair Membership Committee
________________________________________________________________________
___
*Meeting Schedule *
7:30-8:30AM Registration and Continental Breakfast
*
8:30-12:00
*AM Session with Coffee Break
*
Seminars
*
Mouse Models and IHC; Linda Dean
Antibodies 2010; Fatima Natar
*
OR
**
Wet Workshop
*
Multiplex Staining in the Anatomic Laboratory; Tara Kennedy
*
12:00-1:00
*Lunch (wraps, salad, soup, dessert) *
1:00-4:30
*PM Session with Coffee Break *
Seminars
*
CAP Regulations; Terry Murphy
Validation in the IHC Laboratory; George Hoernig
*
OR
**
Wet Workshop
* Rapid In Situ Hybridization; Will
Chappell
------------------------------
Message: 16
Date: Thu, 14 Oct 2010 15:34:27 +1100
From: "Anya Salih" <A.Salih <@t> uws.edu.au>
Subject: [Histonet] Advanced workshop in 3D live cell imaging in
Sydney on 16-19 November.
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<512D5A4F81BF054F9735F9C0AC45AB3B0281BC51 <@t> VIOLA.AD.UWS.EDU.AU>
Content-Type: text/plain; charset="us-ascii"
> You and your students are invited to attend the 3rd Advanced
> Bio-Imaging Workshop at the University of Western Sydney on 16 - 19
> November, 2010 and the Bio-Imaging Expo (free event on 16th November
> 2010)
>
> Tracking Molecules with Light
>
> Training in confocal imaging and protein 3D tracking, aggregation,
> diffusion analyses. Experiments will involve mammalian cell lines,
> invertebrate (coral) cells, plant and algae, bacteria and other
> samples.
>
> Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop.
>
> Places limited to 35 and only 20 places left so register now.
>
>
> Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury
> Campus, University of Western Sydney
> Organiser: Dr Anya Salih
> Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ.
> California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker &
> Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss)
>
>
> Lectures and intensive hands-on training on confocal microscopes by
> top researchers in the field. Learn how to explore and analyse the 3D
> structural complexity of invertebrate, animal & plant cells, tissues
> and micro-organisms, visualize and analyse movement of organelles and
> molecules. Trial a range of novel GFP-type protein constructs. Discuss
> your experiments and trial new approaches. Workshop emphasis on
> advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B,
> FLIM, photoactivatable fluorescent proteins.
>
> Invited speakers
>
> * Professor Enrico Gratton, Director Laboratory for Fluorescence
> Dynamics, University of California, Irvine
> * Professor Takeharu Nagai, Laboratory for Nanosystems
> Physiology & Nikon Imaging Center, Hokkaido University Research
> Institute Electronic Science, Japan
> * Dr. Michelle Digman, Director Optical Biology Core Facility,
> University of California, Irvine
> * A/Professor Guy Cox, Australian Centre for Microscopy &
> Microanalysis, University of Sydney
> * Professor Leann Tilley, Department of Biochemistry, D/Director
> Centre of Excellence in coherent X-ray Science, La Trobe University
> * Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging
> Facility, Garvan Institute of Medical Research, University of Sydney
> * Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute,
> University of Sydney
> * Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin
>
> Training sessions cover the following:
>
> Multi-colour Fluorescent proteins
> Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby,
> pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B
> histone, actin, tubulin, Golgi, membrane); novel Photoactive
> Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from
> reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein
> localization & diffusion.
>
> Confocal Spectral Imaging
> Acquisition of microspectral data (x, y, lambda) in 3D image stacks
> from samples with multiple fluorescent probes or from fluorescent
> coral tissues expressing a variety of GFP-type proteins (A. Salih
> fluorescent corals
> http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he
> alth)- Spectral unmixing, analysis, spectral FRET.
>
> Analysis of molecular movement and diffusion
> Track proteins and other molecules in live cells. Measure protein
> femtoliter concentrations. Monitor mobility and binding using
> fluorescence correlation spectroscopy (FCS), scanning FCS, raster
> image correlation spectroscopy (RICS), number & brightness (N&B) and
> photon counting histogram (PCH).
>
> Fluorescence Lifetime Imaging Microscopy (FLIM)
> - a powerful tool to analyse spatial distribution of excited state
> lifetimes in samples: studied examples will include FRET-FLIM to study
> protein interactions (e.g., DNA-Protein) in cells, imaging of
> photoactivatable FPs, quenching of chlorophyll in plants, etc.
> Hands-on training in FLIM and Phasor FLIM.
>
>
> Workshop microscopes & companies
>
> Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto
> optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B
> Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging,
> photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid
> image acquisition, resonant scanner & 32 channel microspectral
> detection Olymus FluoView FV1000 - variable barrier filter (VBF),
> spectral detection, RICS and N&B
> Ultra VIEW VoX spinning disc confocal microscope, high speed,
> multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments,
> PerkinElmer.
> Confocal FLIM system, Becker & Hickl GmbH, Berlin A range of new
> GFP-type protein constructs of many colours (cyan to far red) linked
> to a variety of cellular proteins for in vivo protein localization
> and dynamic studies, Evrogen And many more other instruments.
>
>
> Registration
>
> Full workshop registration (lectures + training) will be limited to 35
> participants. Registration will be on a first come first served basis.
>
> Contact Anya Salih a.salih <@t> uws.edu.au to reserve your workshop place
> Students $650 (GST inclusive)
> All other $850 (GST inclusive)
>
>
> Attendance of the BioImaging Expo on 16th November does not require
> registration by please rsvp Pamela McMurtry
> [pamela.mcmurtry <@t> theconferenceteam.com.au]
> Accommodation details at registration website, from $260 per week per
> single room at UWS College. Bus shuttle will be available between
> Sydney airport and the workshop venue at UWS campus.
>
> Kind regards,
Anya
Dr Anya Salih
Confocal Bio-Imaging Facility
University Western Sydney
Australia
> 61 2 45701452
> a.salih <@t> uws.edu.au
------------------------------
Message: 17
Date: Thu, 14 Oct 2010 12:07:46 +0100
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] fridge/freezer storage space
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7722595275A4DD4FA225B92CDBF174A1E8DB32C9CC <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"
Does anyone out there have an effective policy for monitoring
the storage of material at 4C,-20C or -80C. At the moment if any
more space is required the solution is usually to buy another
fridge or freezer, which then takes up more floor space, uses more
electricity and quickly fills as individuals use the space that has
become available. So can anyone suggest/ or has in place a system
whereby the contents of fridges/freezers can be monitored on a
regular basis and old/out of date stuff is disposed off. I expect
that all you GLP laboratories have it sorted, and I would
welcome any input from you. Ours is a university lab where no such
rules/regulations seem to exist and often when people leave they
forget to throw out their stuff which can then remain for years.
Many thanks
Richard Edwards
Leicester
University
U.K.
------------------------------
Message: 18
Date: Thu, 14 Oct 2010 06:49:54 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and
ER/PR
To: "'Amos Brooks'" <amosbrooks <@t> gmail.com>, <JEllin <@t> yumaregional.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <26E0799EA0D8454A86A3F86DF8A6F22D <@t> prueggihctechlt>
Content-Type: text/plain; charset="us-ascii"
Here here Amos. This is why I am for using multi tissue controls that
have
considered a range for most of the differences in tissues, fixation and
processing encountered.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos
Brooks
Sent: Wednesday, October 13, 2010 4:21 PM
To: JEllin <@t> yumaregional.org; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
Hi,
I'm going to have to disagree with this approach. Lumping all
specimens
into the same procedure (fixation/processing time) is in no way a step
toward individualized care that is so often discussed. Doing this
ignores
the basic fundamental differences between the specimens. A liver is
different from a breast and a brain. Likewise a particularly fatty
breast is
different from a fiberous one, or one that is cut smaller than the one
the
other pathologist (or PA) stuffed into a cassette. Each of these
situations
needs to be addressed differently from fixation to processing times and
to
be entirely honest staining times in many cases. I fear you may be
oversimplifying the situation and calling it standardization.
All the best,
Amos
Date: Wed, 13 Oct 2010 08:05:27 -0700
From: "Jesus Ellin" <JEllin <@t> yumaregional.org>
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
To: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>, "Phyllis
Thaxton"
<dchihc <@t> yahoo.com>, "Mahoney,Janice A" <
Janice.Mahoney <@t> alegent.org>,
"Mike Pence" <mpence <@t> grhs.net>, "Joyce Cline"
<Joyce.Cline <@t> wchsys.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<29BE166A2CF48D459853F8EC57CD37E8021C68C9 <@t> EXCHANGECLUSTER.yumaregional.l
ocal
>
Content-Type: text/plain; charset="US-ASCII"
OK I usually do not like to chime in on this, but here I go. How can a
true validation of a specific target be obtained if the wiggle room is 6
to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the
results for this testing if you are going to be varying process for the
weekend vs weekday? This is the flaw in the guidelines in my
perspective, when this much time is allowed it is like anything else.
We are going to go the path of least resistance to change, instead of
what is right. I know that as techs we always want the best, but are
pushed to produce next day. Techs for years have been saying more
fixation is needed on tissue. Well enough of that.
What we do is we hold at 12 hours of fixation for all specimens no
matter what? We document ischemic cold time through our LIS, to include
time placed in formalin, and time of first cut. We feel that all
specimens need the same fixation times. This is imperative to
standardize the process, but once again we also have our processors set
up in such a way that they come off at different times and our
production of H an E is in sync with this. It might sound like a lot,
but we get most of our work done around 96 to 97 % of cases within 24
hours or less using conventional processing techniques.
With the future relying more and more on, patient centered care, through
personalized medicine, we need to really look on how we can do the
optimal requirements, not do the minimal requirements to reach our
goals.
Jesus Ellin
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Thu, 14 Oct 2010 08:58:25 -0400
From: John Shelley <jshelley <@t> sanfordburnham.org>
Subject: [Histonet] RE: fridge/freezer storage space
To: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CEDED70E7CD1FD418B1525ABD333554F7BF2078D9A <@t> LN-MAIL07.ln.burnham.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi Richard,
We are a research facility and have the same issue with not always
knowing where and whose stuff is in the freezer or refrigerators. Even
with the best system though you still have to have people who
communicate that they are taking stuff out and also replacing back where
it was removed from.
We have instituted the use of a system called Freezer Pro. Here is the
web address http://www.ruro.com/products/freezerpro.html
At least this is a start, hope it helps.
Kind Regards!
John J Shelley
Senior Research Associate, Histology Core Facility
Sanford-Burnham Medical Research Institute at Lake Nona
6400 Sanger Road
Orlando, FL 32827
Tel: (407) 745-2000 Ext.2517
Fax: (407) 745-2001
email: jshelley <@t> sanfordburnham.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Edwards,
Richard E.
Sent: Thursday, October 14, 2010 7:08 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fridge/freezer storage space
Does anyone out there have an effective policy for monitoring
the storage of material at 4C,-20C or -80C. At the moment if any
more space is required the solution is usually to buy another
fridge or freezer, which then takes up more floor space, uses more
electricity and quickly fills as individuals use the space that has
become available. So can anyone suggest/ or has in place a system
whereby the contents of fridges/freezers can be monitored on a
regular basis and old/out of date stuff is disposed off. I expect
that all you GLP laboratories have it sorted, and I would
welcome any input from you. Ours is a university lab where no such
rules/regulations seem to exist and often when people leave they
forget to throw out their stuff which can then remain for years.
Many thanks
Richard Edwards
Leicester
University
U.K.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 83, Issue 20
****************************************
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
More information about the Histonet
mailing list