SPAM-LOW: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

[Patsy Ruegg]

The problem here is that the studies have not been done or at least not
published to say if 6-72 hours fixation in formalin is what we should be
using for ER/PR/Her2.  The ASCO/CAP Guidelines for ER/PR (not Her2) were
based upon literature searches not lab data.  The only reference I found
regarding not extending fixation past 72 hours was from Anthony Leon in
1998, pre antigen retrieval and pre the literature search for the guidelines
which limited themselves to literature published after 2000.  It would be
nice if the guidelines set forth by ASCO/CAP were actually based upon data
tested scientifically but it is not.  It is up to individual labs to do
their own studies and if possible have low expression control tissue that is
fixed at intervals from 6-72 hours which can be used as control indications
for what ever fixation time we come across.  My two sense worth.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kuhnla,
Melissa
Sent: Tuesday, October 12, 2010 11:03 AM
To: Mahoney,Janice A; Mike Pence; Joyce Cline;
histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

I disagree.  Prolonged formalin fixation (over 48 hrs), diminishes
signals

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 12:05 PM
To: 'Mike Pence'; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

Formalin fixation time does not impact the results of FISH as it does
IHC.
Jan M
Omaha

-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net]
Sent: Tuesday, October 12, 2010 11:00 AM
To: Mahoney,Janice A; Joyce Cline; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

I don't think it matters if you do Her2 by FISH or IHC the time is still
48hr. I hope I am wrong, but I don't think I am.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 10:25 AM
To: 'Joyce Cline'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR


We have decided to reflex to FISH those breasts that do not fall within
the recommended formalin fixation time.  We do work on Saturdays so it
is only the rare 3 day weekends that this comes into play. Jan M Omaha

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: Tuesday, October 12, 2010 10:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR

Does anyone have any experience with storing formalin fixed breast
tissue in 70% before processing?  I am trying to comply with the new
guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and
since my lab does not operate on the weekend we have been well above the
48 hour recommended formalin fixation time.  Does 70% affect
antigenicity for either Her2 or ER/PR?  Any information or suggestions
will be greatly appreciated.  Thanks :)


Ronda Souders
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>

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