[Histonet] Bone IHC

Liz Chlipala liz <@t> premierlab.com
Thu Oct 7 14:20:23 CDT 2010

We lower the temp of retrieval to 70C for 2 hours and have good success
with that.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vanessa
J. Phelan
Sent: Thursday, October 07, 2010 1:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bone IHC

Hi everyone,

I was wondering if anyone has any tricks on how to get bone sections to
stop lifting off the slide through the IHC process? I leave them in the
oven for quite a while to make sure they are baked on, however after
antigen retrieval  (pressure cooker for 20mins) most of the boney part
of the tissue comes off and the marrow and muscle stays put! The
sections are cut onto superfrost plus slides.

Any help would be much appreciated, thanks.

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