[Histonet] Training
Carter, Kendra
CarterK <@t> MedImmune.com
Thu Oct 7 08:24:23 CDT 2010
Does anyone know of any GLP training in El Paso, TX?
Kendra Leigh Carter
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, October 06, 2010 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 83, Issue 6
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Today's Topics:
1. RE: Helicobacter pylori (Laurie Colbert)
2. RE: AChE fiber staining protocoll? (szigcs <@t> bio.u-szeged.hu)
3. RE: Helicobacter pylori (Weems, Joyce)
4. PAS STAIN (Diana McCaig)
5. Histokinette 2000 (Biedermann, JoAnn)
6. RE: PAS STAIN (Rathborne, Toni)
7. Query Special Stainers (Joanne Clark)
8. Re: Helicobacter pylori (Brandi Higgins)
9. AW: [Histonet] PAS STAIN (Gudrun Lang)
10. Where to buy Michels RE: [Histonet] DIF Transport Media
(gayle callis)
11. RE: Query Special Stainers (Mahoney,Janice A)
12. RE: PAS STAIN (gayle callis)
13. RE: Query Special Stainers (Marcia Funk)
14. PAS staining (Janet Keeping)
----------------------------------------------------------------------
Message: 1
Date: Wed, 6 Oct 2010 07:48:25 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: RE: [Histonet] Helicobacter pylori
To: "Kathy M. Gorham" <KMB01 <@t> grh.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<57BE698966D5C54EAE8612E8941D768309A5ACBC <@t> EXCHANGE3.huntingtonhospital.com>
Content-Type: text/plain; charset="us-ascii"
We basically do a dif quik stain. I buy a kit called "Three Step Stain"
from Cardinal. The total staining process takes about 1 minute.
Laurie Colbert
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kathy M.
Gorham
Sent: Wednesday, October 06, 2010 7:20 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Helicobacter pylori
Good Morning Histo land. I would like to know what others are using for
a special stain for Helicobacter pylori not IHC. Do you use a kit? From
where? Make up your own? Procedure. Thanks you. You have always been
so helpful. Kathy Gorham H.T.
GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS
Financial Excellence Award 2010 awarded by the national Rural Health
Research & Policy Analysis Center
Healthcare Achievement Award for Quality in Patient Care Delivery and
Satisfaction 2010 awarded by Amerinet
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We will ensure access to high-quality, cost-effective health services in
a safe, customer-friendly environment for all those in need of our
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Message: 2
Date: Wed, 06 Oct 2010 17:00:35 +0200
From: szigcs <@t> bio.u-szeged.hu
Subject: RE: [Histonet] AChE fiber staining protocoll?
To: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20101006170035.j2waztulso4480gg <@t> webmail.u-szeged.hu>
Content-Type: text/plain; charset=ISO-8859-2; DelSp="Yes";
format="flowed"
Idézet ("Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>):
Thank you for the answer. We will try this protocol and i will reply
the results.
Csaba Szigeti
> I have had great success in fine fibers with the modification of
> Martucciello et al, Eur J Pediatr Surg 2001; 11: 300-304, but only
> in 15 micron intestine sections, so I do not know how it will
> transpose to brain studies and the thicker sections
>
> Ronnie Houston
> Anatomic Pathology Manager
> Nationwide Children's Hospital
> Columbus OH 43205
> (614) 722 5450
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> szigcs <@t> bio.u-szeged.hu
> Sent: Wednesday, October 06, 2010 3:34 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] AChE fiber staining protocoll?
>
> Dear Histonet Members!
>
> We are going to investigate AChE fiber density changes in the cortex
> of transcardially perfused rat brain slices (30 micrometer, criostat
> sections, perfusing solution: 4% formaldehyde in PB ph 7.4). We need a
> protocoll to visualize FINE fiber structure to be able to count changes.
>
> We used modification of Hedreen and original Tago protocoll. Hedreen
> good results for general, but no fiber staining, Tago no sucsses.
>
> Could you please tell us some advise, protocolls functioning on teh
> above mentioned objects?
>
> Thank you all in advance...
> Csaba Szigeti
>
> ----------------------------------------------------------------
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Message: 3
Date: Wed, 6 Oct 2010 11:01:52 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: Helicobacter pylori
To: "Kathy M. Gorham" <KMB01 <@t> grh.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E1640398777983 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
We use what we call a modified Genta. That is a modified Steiner if done manually. Because we have the Artisan and DAKO didn't have a Genta kit, I tried using the Warthin Starry and adding the Alcian blue and H&E manually and it worked fine. So we have not renamed it a modified Warthin Starry! But the H. pylori are very visible as well as the mucin and the morphology. J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham
Sent: Wednesday, October 06, 2010 10:20
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Helicobacter pylori
Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T.
GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet
GRH Mission
We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services.
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Message: 4
Date: Wed, 6 Oct 2010 11:03:05 -0400
From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] PAS STAIN
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DCFD9E6A390E294AAF3A2561CD32E5C4139D8C97 <@t> ckhamail1.ckha.on.ca>
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Hi
We have been doing a PAS stain on the same control block and same
reagent supplier for a long time.
Yesterday when we ran the slides, they failed to stain
We opened new bottles of Periodic Acid and Schiff's Reagent and recut
fresh controls
Still, we are unable to get any staining.
Suggestions to help would be appreciated
Diana
------------------------------
Message: 5
Date: Wed, 6 Oct 2010 10:04:55 -0500
From: "Biedermann, JoAnn" <JABiedermann <@t> uams.edu>
Subject: [Histonet] Histokinette 2000
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<833890C7E1BE584B926A2584E6B37ADC6B03F42E <@t> MAIL2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
I have a Leica Histokinette 2000 that needs one beaker too be usable. Does anyone know where I can buy one of these? IMEB, inc does not have them.
Jo Ann Biedermann
Research Assistant
University of Arkansas for Medical Sciences
Reynolds Institute on Aging
629 Jack Stephens Drive
Room 3173 Mail Slot 807
Little Rock, AR 72205
Phone: 501-526-5803
FAX: 501-526-5830
JABiedermann <@t> uams.edu<mailto:JABiedermann <@t> uams.edu>
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Message: 6
Date: Wed, 6 Oct 2010 11:06:12 -0400
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] PAS STAIN
To: "Diana McCaig" <dmccaig <@t> ckha.on.ca>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E78340C766A5284D999F5F5891DDF890144747B7 <@t> smcmail.somerset-healthcare.com>
Content-Type: text/plain; charset="utf-8"
Were the new bottles from the same lot?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Diana
McCaig
Sent: Wednesday, October 06, 2010 11:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PAS STAIN
Hi
We have been doing a PAS stain on the same control block and same
reagent supplier for a long time.
Yesterday when we ran the slides, they failed to stain
We opened new bottles of Periodic Acid and Schiff's Reagent and recut
fresh controls
Still, we are unable to get any staining.
Suggestions to help would be appreciated
Diana
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Message: 7
Date: Wed, 6 Oct 2010 09:22:12 -0600
From: "Joanne Clark" <jclark <@t> pcnm.com>
Subject: [Histonet] Query Special Stainers
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01375230 <@t> mail.pcnm.com>
Content-Type: text/plain; charset="us-ascii"
Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use
for our IHC. We now have a different IHC platform that we use and would
like to change the Ventana over to use for running our special stains.
Does anyone use the Ventana for their specials and have any advice or
comments about how it performs? Thanks!
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
------------------------------
Message: 8
Date: Wed, 6 Oct 2010 11:27:59 -0400
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: Re: [Histonet] Helicobacter pylori
To: "Kathy M. Gorham" <KMB01 <@t> grh.org>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTim=jFxk+Fh+vHs-zHw=oW9KiLu0NoRMU89W9KZY <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello,
We use what is pretty much a diff quik stain, the stain is called QW and it
is from poly scientific. The procedure is really easy. Deparaffinize,
alcohols, methanol, water and then we do 15 slow dips in the QW2, 15 slow
dips in the QW3, wash in water, 2 quick dips in alcohol and then dry in the
oven, coverslip. Depending on your pathologist's preference you may need to
adjust the dips in QW3 or the dips in the alcohol (which will decolorize
slightly). Hope this helps!
Brandi Higgins, BS, HT(ASCP)
On Wed, Oct 6, 2010 at 10:19 AM, Kathy M. Gorham <KMB01 <@t> grh.org> wrote:
> Good Morning Histo land. I would like to know what others are using for
> a special stain for Helicobacter pylori not IHC. Do you use a kit? From
> where? Make up your own? Procedure. Thanks you. You have always been
> so helpful. Kathy Gorham H.T.
>
>
> GRH National Recognition
> Outstanding Rural Health Organization of 2009 awarded by NRHA
> Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
> Leader in Innovative Excellence 2009 awarded by the OAHHS
> Financial Excellence Award 2010 awarded by the national Rural Health
> Research & Policy Analysis Center
> Healthcare Achievement Award for Quality in Patient Care Delivery and
> Satisfaction 2010 awarded by Amerinet
>
> GRH Mission
> We will ensure access to high-quality, cost-effective health services in a
> safe, customer-friendly environment for all those in need of our services.
>
>
> GRH Confidentiality Notice
> This e-mail and any attached documents are for the intended recipient/s
> only
> and should be protected against viewing by unauthorized persons. The
> information
> herein may have been disclosed from records whose confidentiality is
> protected
> by Federal and State Law. Federal regulations prohibit further distribution
> or
> copying of this information without permission. If you received this
> e-mail
> transmission in error, please notify the sender immediately to arrange for
> return
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------------------------------
Message: 9
Date: Wed, 6 Oct 2010 17:39:03 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] PAS STAIN
To: "'Diana McCaig'" <dmccaig <@t> ckha.on.ca>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <986B2C6B449344EBAECEB4080A6E542D <@t> dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
You can test the activity of the Schiffs. Add a few drops of formalin to a
small amount of Schiffs. There should be the typical pink stain.
The pH should be about 2. If there are white flakes in the bottle, this
could be a sign for a too high pH.
Is your periodic acid ok?
Gudrun
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Diana
McCaig
Gesendet: Mittwoch, 06. Oktober 2010 17:03
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] PAS STAIN
Hi
We have been doing a PAS stain on the same control block and same
reagent supplier for a long time.
Yesterday when we ran the slides, they failed to stain
We opened new bottles of Periodic Acid and Schiff's Reagent and recut
fresh controls
Still, we are unable to get any staining.
Suggestions to help would be appreciated
Diana
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Wed, 6 Oct 2010 09:40:17 -0600
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: Where to buy Michels RE: [Histonet] DIF Transport Media
To: "'Tony Henwood'" <AnthonyH <@t> chw.edu.au>, "'Della Speranza, Vinnie'"
<dellav <@t> musc.edu>, "'kristen arvidson'" <arvidsonkristen <@t> yahoo.com>,
"'histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002801cb656c$c8bd8cd0$5a38a670$@callis <@t> bresnan.net>
Content-Type: text/plain; charset="iso-8859-1"
Dear Tony,
Thank you for the fine reply on Michel's Transport Media. We had excellent
results with human renal biopsies destined for immunofluorescence staining.
I don't recall ever exceeding 48 hours in Michels as 72 hours was allowed
per recommendation from the original and Elias's publications. The kidney
morphology didn't suffer excessively from this transport media as seen on
our H&E stained frozen sections from Liquid Nitrogen cooled isopentane snap
frozen needle biopsies. My renal pathologist always commented that the
frozen section H&E looked very much like his the FFPE H&E section. This must
vary from laboratory to laboratory and also what tissue was transported e.g.
skin biopsies versus kidney biopsies. We always were very careful with good
Michels buffer rinses.
As for where to access Michels Transport Media and Michels buffer, we
purchased these from Poly Scientific in much larger volumes at a cheaper
price than Wampole(?)(Zeus). Aliquots were made and distributed to
laboratories although that may not be ideal since busy laboratories may find
this a bit labor intensive. I know of laboratories who make up Michels with
great success.
As for transporting tissue on saline soaked gauze, I can't stress the
importance enough to NEVER let the tissue dry out, and snap freeze it as
soon as it arrives in the laboratory. I would prefer receiving a tissue in
cell culture fluid to ensure no drying.
The reference is much appreciated.
G'day and missed seeing you at NSH symposium/convention this year.
Gayle M. Callis
HTL/HT/MT(ASCP)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: Tuesday, October 05, 2010 5:44 PM
To: Della Speranza, Vinnie; kristen arvidson; histonet
Subject: RE: [Histonet] DIF Transport Media
Vinnie,
I hope you are well.
The following might be of use:
Specimens for immunofluorescence are usually submitted to the laboratory in
cell culture fluid (eg Hanks or RPMI) or on saline soaked gauze. For
transport to other institutions, Michel's Transport Medium has often been
used:
Michel's Buffer
1M potassium citrate, pH 7.0 2.5 ml
0.1M magnesium sulphate 5.0 ml
0.1M N ethylmalemide 5.0 ml
Distilled H2O 87.5 ml
* Mix well and store at 2 8oC. Exp. 1 year
Michel's Transport Medium
Michel's Buffer 100ml
Ammonium sulphate 55gm
Adjust pH to 7.0 7.4 with 1M KOH. Store at 2 8oC. Exp. 1 year
Unfortunately Michel's Transport Medium has erroneously been called Michel's
Fixative. None of the components of Michel's Transport Medium is a fixative.
Ammonium sulphate precipitates antigen-antibody complexes in diseased skin
and renal tissues. N ethylmalemide modifies free sulphydryl groups of
cysteine residues in proteins (Fischer 2006).
Michel's Transport Medium has been shown to be deleterious to morphology.
Ultrastructurally, complete destruction of plasma membranes and
intracytoplasmic organelles occurs after 48 hours storage. On the other
hand, antigenicity is well preserved even after many days storage (Fischer
2006).
Fischer (2006) Intern J Surg Pathol 14(1):108.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Della
Speranza, Vinnie
Sent: Wednesday, 6 October 2010 6:10 AM
To: 'kristen arvidson'; histonet
Subject: RE: [Histonet] DIF Transport Media
Zeus was a company that used to market michel's under their own label. I
don't believe zeus still exists. Michel's is the name associated with the
author of the original paper. I don't have the reference.
This solution does not require refrigeration.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Monday, October 04, 2010 2:30 PM
To: histonet
Subject: [Histonet] DIF Transport Media
Is Michel Medium the same as Zeus? Do these need to be refrigerated?
Thanks.
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Message: 11
Date: Wed, 6 Oct 2010 10:49:22 -0500
From: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Subject: [Histonet] RE: Query Special Stainers
To: 'Joanne Clark' <jclark <@t> pcnm.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8F0EE4144E8E2F4CA1F6B051A2E5BFEE029CA43F40 <@t> EXCHMBC2.ad.ah.local>
Content-Type: text/plain; charset="us-ascii"
We have 2 Ventana special stainers and they are very reliable work horses. They run every day over half the day and put out very consistent results. Very user friendly too.
Jan M
Omaha
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Wednesday, October 06, 2010 10:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Query Special Stainers
Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use
for our IHC. We now have a different IHC platform that we use and would
like to change the Ventana over to use for running our special stains.
Does anyone use the Ventana for their specials and have any advice or
comments about how it performs? Thanks!
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
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------------------------------
Message: 12
Date: Wed, 6 Oct 2010 10:04:44 -0600
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: RE: [Histonet] PAS STAIN
To: "'Rathborne, Toni'" <trathborne <@t> somerset-healthcare.com>, "'Diana
McCaig'" <dmccaig <@t> ckha.on.ca>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003401cb6570$33037c00$990a7400$@callis <@t> bresnan.net>
Content-Type: text/plain; charset="utf-8"
I suspect bad Periodic acid. We never buy periodic acid already in solution and if that has been sitting around even in a kit, it may have gone bad. In a workshop taught by Charles Culling, an expert on PAS staining, he stressed making periodic acid fresh daily or each time the stain is done. It is not expensive, goes into solution readily, then toss the periodic acid to never be reused with exception of that one day. Also, you can test your Schiffs reagent by putting a few drops of Schiffs into 10 mls NBF, watch it turn a very bright pink red instantly. If it turns purplish, it is not good. It may be a bad kit, but you never said you were using a kit only "new bottles of ....."
Consequently we buy periodic acid in crystalline form, make up 1% Periodic acid, and buy the Schiffs Reagent from Fisher, never a kit. Sigma also sells good Schiffs, but Fisher Scientific aka Thermo has larger quantity for less price. We also store our Schiffs in the refrigerator rather than room temperature, a habit left over from days when we made this reagent up in house.
Gayle M. Callis
HTL/HT/MT(ASCP)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, October 06, 2010 9:06 AM
To: Diana McCaig; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] PAS STAIN
Were the new bottles from the same lot?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Diana
McCaig
Sent: Wednesday, October 06, 2010 11:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PAS STAIN
Hi
We have been doing a PAS stain on the same control block and same
reagent supplier for a long time.
Yesterday when we ran the slides, they failed to stain
We opened new bottles of Periodic Acid and Schiff's Reagent and recut
fresh controls
Still, we are unable to get any staining.
Suggestions to help would be appreciated
Diana
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Message: 13
Date: Wed, 06 Oct 2010 12:08:04 -0400
From: "Marcia Funk" <FUNKM <@t> mercyhealth.com>
Subject: [Histonet] RE: Query Special Stainers
To: "Janice A Mahoney" <Janice.Mahoney <@t> alegent.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "'Joanne Clark'"
<jclark <@t> pcnm.com>
Message-ID: <4CAC5893.9B87.00AC.0 <@t> mercyhealth.com>
Content-Type: text/plain; charset=US-ASCII
We also have 2 Vantnan Special stainers and totally agree.
Marcia M
mason City
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907
>>> "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org> 10/06/2010 10:49 AM >>>
We have 2 Ventana special stainers and they are very reliable work horses. They run every day over half the day and put out very consistent results. Very user friendly too.
Jan M
Omaha
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Wednesday, October 06, 2010 10:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Query Special Stainers
Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use
for our IHC. We now have a different IHC platform that we use and would
like to change the Ventana over to use for running our special stains.
Does anyone use the Ventana for their specials and have any advice or
comments about how it performs? Thanks!
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person.
The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation.
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------------------------------
Message: 14
Date: Wed, 6 Oct 2010 14:13:23 -0230
From: Janet Keeping <jankeeping <@t> gmail.com>
Subject: [Histonet] PAS staining
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTikwzC9T3oc4TxTMOMnBJy-n=VEa_Q+KOOtpWN3z <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I have for some time had a problem with Schiff's reagent and PAS staining.
- I have tested each new, unopened bottle of Schiff's reagent with
formaldehyde and always the color development was immediate, but purple,
definately not pink.This result has been quite consistant.
- PAS staining for glycogen using the method in *Histotechnology a self
Instructional text,* would fail to demonstrate any glycogen in autopsy
liver specimens.
I teach Histology at a community college and this problem has driven me
crazy for a number of years. I have tried several brands of Schiff with the
same results. Recently I obtained sheep tissues which were promptly
refrigerated and fixed after death, and I had hoped these tissues would
demonstrate glycogen. ( My thinking was that perhaps delay before autopsy
was somehow diminishing the glycogen in the specimens that I had.or that
perhaps long term NBF fixation had hampered staining.) Basement membranes
were stained with the Schiff reagent as expected despite the purple color in
the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced
beautiful staining of fungus a lovely magenta color. A search of the web
made me suspiciious when I noted that Schiff added to 37-40% formaldehyde
should produce a pink or red color, however, A spot check of formalin using
Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to
ask if he could make any recommendation.
Brian was not surprised by the purple color devopment in testing, He
suggested that a large number of available aldehyde groups would be expected
to produce this color. He suggested that I increase my periodate oxidation
to 20-30 minutes and my Schiff application to 30 minutes.
This worked and I am extremely grateful!. Has anyone else had an experience
like this?
Janet
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End of Histonet Digest, Vol 83, Issue 6
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