[Histonet] Question about Oil Red O controls

Andrea Grantham algranth <@t> email.arizona.edu
Wed Oct 6 14:42:11 CDT 2010

I don't know what kind of lab you are in, I'm in a core facility and I  
do histology on research projects. When I get an ORO this is what I do  
for a control:

I get a piece of tissue like mouse kidney with some fat attached or  
maybe some muscle with fat and have it snap frozen. I have found that  
the frozen blocks stay good for a long time at
-80ºC and so do the frozen sections on slides. I always cut a bunch of  
slides and store them and take one out when I have the stain ordered.


Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello
P Please consider the environment before printing this email.

On Oct 6, 2010, at 6:15 AM, Komal Gada wrote:

> Hello Histonetters,
> I am trying to find a procedure for using butter and egg yolks as  
> controls
> for the Oil Red O stain (to show the fat).
> Does anyone have something they would be able to share with me?
> Thanks,
> Komal
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