[Histonet] Osteoclast

[McMahon, Loralee A]

We were never able to get the TRAP working with Formic Acid decal.  Using a home brewed TRAP kit or the Sigma Kit.  
But Cell Marque has a TRAcP antibody (catalog 341M-95) that is used as a hairy cell marker.  But it also stains macrophages and osteoclasts.  

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Praveen Arany [arany <@t> fas.harvard.edu]
Sent: Wednesday, October 06, 2010 9:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Osteoclast

  Hi Loralee,
I am having similar problems with Formic acid Decal'd sections for TRAP.
After EDTA, are you doing a TRAP IHC or the enzyme assay from Sigma?
As for molecular markers, Cathepsin K is pretty good for Osteoclasts but
I do westerns in a research setting......has anyone had good success
with any antibody for immunostaining?
Best,

Praveen Arany,
Graduate Student,
Harvard University,
Cambridge MA

On 10/6/2010 8:43 AM, McMahon, Loralee A wrote:
> You can do a TRAP stain on EDTA decaled bone.  I haven't been able to get it to work on formic acid decaled bone.  I also believe that there is an antibody for osteoclasts, but I have not tried it.
>
> Loralee McMahon, HTL (ASCP)
> Immunohistochemistry Supervisor
> Strong Memorial Hospital
> Department of Surgical Pathology
> (585) 275-7210
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lin Bustamante [lbustamante <@t> cvm.tamu.edu]
> Sent: Tuesday, October 05, 2010 4:58 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Osteoclast
>
> We need to find a way to stain mainly Osteoclast.
> Any suggestions?
> Thank you very much.
> Lin
>
> Lin S. Bustamante, B.Sc.; HT(ASCP)
> Research Associate
> Histology Lab Supervisor
> Veterinary Integrative Bioscience
> Texas A&M University
> College Station, TX 77843-4458
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