[Histonet] DIF Transport Media

[Tony Henwood]

Vinnie,

I hope you are well.

The following might be of use:

Specimens for immunofluorescence are usually submitted to the laboratory in cell culture fluid (eg Hanks or RPMI) or on saline soaked gauze. For transport to other institutions, Michel's Transport Medium has often been used:

Michel's Buffer
1M potassium citrate, pH 7.0		2.5 ml
0.1M magnesium sulphate  		5.0 ml
0.1M N ethylmalemide       		5.0 ml
Distilled H2O 				87.5 ml
* Mix well and store at 2 8oC.   Exp. 1 year

Michel's Transport Medium
Michel's Buffer 			100ml
Ammonium sulphate  			55gm
Adjust pH to 7.0 7.4 with 1M KOH.  Store at 2 8oC.  Exp. 1 year

Unfortunately Michel's Transport Medium has erroneously been called Michel's Fixative. None of the components of Michel's Transport Medium is a fixative. Ammonium sulphate precipitates antigen-antibody complexes in diseased skin and renal tissues. N ethylmalemide modifies free sulphydryl groups of cysteine residues in proteins (Fischer 2006). 

Michel's Transport Medium has been shown to be deleterious to morphology. Ultrastructurally, complete destruction of plasma membranes and intracytoplasmic organelles occurs after 48 hours storage. On the other hand, antigenicity is well preserved even after many days storage (Fischer 2006).

Fischer (2006) Intern J Surg Pathol 14(1):108.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie
Sent: Wednesday, 6 October 2010 6:10 AM
To: 'kristen arvidson'; histonet
Subject: RE: [Histonet] DIF Transport Media


Zeus was a company that used to market michel's under their own label. I don't believe zeus still exists. Michel's is the name associated with the author of the original paper. I don't have the reference.

This solution does not require refrigeration.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Monday, October 04, 2010 2:30 PM
To: histonet
Subject: [Histonet] DIF Transport Media

Is Michel Medium the same as Zeus?  Do these need to be refrigerated? Thanks.


      
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