[Histonet] Re: Histonet Digest, Vol 83, Issue 4

Stephanie Rodriguez srodriguez <@t> phenopath.com
Tue Oct 5 12:56:09 CDT 2010

Barb, Eric, all-

We also use a diamond pen/pencil-and they do contain an industrial grade
diamond, so they are quite reasonably priced; I looked one up on the VWR
website (just as a reference, not necessarily an endorsement! :o) and they
run about $20-30 each.  They last a very long time, too-We¹ve had ours for
several years.

We use them for our direct IFs, FISH, anything dark field.


Stephanie Rodriguez, HTL(ASCP), QIHC
Lead Molecular Technologist-FISH
IHC Technologist III
Phenopath Laboratories
Seattle, WA
(206) 374-9000 

On 10/5/10 10:15 AM, "histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu> wrote:

> Message: 11
> Date: Tue, 5 Oct 2010 10:53:36 -0400
> From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
> Subject: [Histonet] Direct immunofluorescence question
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>  <F93BD6329FC3AE4C8DB116B985FBC31327C3ACAC <@t> KGHMAIL.KGH.ON.CA>
> Content-Type: text/plain; charset="iso-8859-1"
> Barb,
> Have you tried a diamond pencil?  Available from a variety of sources, these
> "pencils" can be used to etch a circle or other shape around the tissue to be
> viewed.  I believe they use lesser-quality industrial diamonds to make the
> tips on the pencils (i.e. not the diamond ring quality). Coming across the
> etched line under the fluorescent microscope produces brightness that helps
> the pathologist find the tissue.
> We circle our DIF's on the back of the slide - it won't wash off and won't
> interfere with reagents this way, but is still visible in a darkened room.
> Hope this helps,
> Eric Gagnon MLT
> Histology Laboratory
> Kingston General Hospital
> Kingston, Ontario, Canada


Message: 1
Date: Mon, 4 Oct 2010 12:49:44 -0500
From: bamoe <@t> gundluth.org
Subject: [Histonet] Direct immunofluoresent question
To: histonet <@t> lists.utsouthwestern.edu
    <OF9419A09E.870A453A-ON862577B2.00614E6B-862577B2.0061EF73 <@t> gundluth.org>
Content-Type: text/plain; charset=US-ASCII

Hi all -

The pathologist that reads our direct IF slides likes to have the sections
of tissue circled on the slide so that they are easier to find.  We
currently use black marker on the back of the slide, but find that many
times it smears and are looking for a better solution.

What kind of slides do others use, and if you circle your sections what
marker/pen/etcher do you use?

Any thoughts are greatly appreciated!

Barb Moe
Gundersen Lutheran Medical Center
La Crosse WI

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