[Histonet] Direct immunofluoresent question

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Oct 4 14:21:26 CDT 2010

I used to mark on the slide, around the section, with a wax marker. Once dried and the procedure completed, cover and observe.
René J.

--- On Mon, 10/4/10, bamoe <@t> gundluth.org <bamoe <@t> gundluth.org> wrote:

From: bamoe <@t> gundluth.org <bamoe <@t> gundluth.org>
Subject: [Histonet] Direct immunofluoresent question
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, October 4, 2010, 1:49 PM

Hi all -

The pathologist that reads our direct IF slides likes to have the sections
of tissue circled on the slide so that they are easier to find.  We
currently use black marker on the back of the slide, but find that many
times it smears and are looking for a better solution.

What kind of slides do others use, and if you circle your sections what
marker/pen/etcher do you use?

Any thoughts are greatly appreciated!

Barb Moe
Gundersen Lutheran Medical Center
La Crosse WI

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