[Histonet] carnoys fluid

IRENA SREBOTNIK KIRBIS irena.kirbis <@t> hotmail.com
Thu Nov 25 01:40:33 CST 2010


I would recommend methanol or ethanol for fixation, smears itself can be culprit of inconsistent staining
Irena
> To: histonet <@t> lists.utsouthwestern.edu
> From: heather.mcleod <@t> webmail.co.za
> Date: Wed, 24 Nov 2010 19:42:51 +0200
> Subject: [Histonet] carnoys fluid
> 
> Dear Histonetters,
> 
> We do IHC on pap stained cyto smears. The results do not always "behave"
> accordingly on the smears that have been exposed to Carnoys. Does anybody know
> whether Carnoys Fluid affects IHC on cytology smears? I have seen papers that
> suggest improved IHC after Carnoys but maybe Histonetters feel difefrently?
> Thanks 
> Heather
> 
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