[Histonet] Help cutting horse tendon.

Pablo Sanchez-Quinteiro pablo.sanchez <@t> usc.es
Wed Nov 24 13:09:13 CST 2010


Dear listers,

I am trying to cut whole sections of the tendon of the digital flexor 
muscle of the horse. The piece is around 1 mm long and very elastic 
and fibrous. I have tried formalin and Bouin fixation, the latest 
better. With paraffin embedding the fibrous elements of the tendon 
spoil the sections. In freezing microtome the sections seem nice but 
the interfascicullar spaces are too wide at the microscope.

I would very grateful for any hint, suggestion, etc.

Best regards,

Pablo




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