[Histonet] Help cutting horse tendon.
pablo.sanchez <@t> usc.es
Wed Nov 24 13:09:13 CST 2010
I am trying to cut whole sections of the tendon of the digital flexor
muscle of the horse. The piece is around 1 mm long and very elastic
and fibrous. I have tried formalin and Bouin fixation, the latest
better. With paraffin embedding the fibrous elements of the tendon
spoil the sections. In freezing microtome the sections seem nice but
the interfascicullar spaces are too wide at the microscope.
I would very grateful for any hint, suggestion, etc.
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