SPAM-LOW: [Histonet] glutaraldehyde fixed tissues and genomic DNA

Patsy Ruegg pruegg <@t>
Sun Nov 21 12:52:11 CST 2010

Wow this sounds like a molecular biology question that will have to be
figured out and probably no one has done that yet for this particular
situation.  Please write it up if you do figure it out.


Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. Ste.215
Aurora, CO 80045
fax 720-859-4110

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Subject: SPAM-LOW: [Histonet] glutaraldehyde fixed tissues and genomic DNA


I have PFA+glutaraldehyde fixed tissues that I might want to digest down
in order to extract genomic DNA suitable for bisulfite sequencing.

The tissues are embryonic mouse heads. The fixative is a PFA +
glutaraldehyde mix:  3.6 PFA with 5% glut. The glut is grade 1 or electron
miccroscopy grade.

I'd like to pro-K the tissues and then phenol-chloroform extract genomic

My hope is that the amount and quality of the DNA will be suitable for
bisulfite conversions for methylation anaylsis. Will the DNA be abundant?
Will it be fairly protein free (does the fixative fix protein to the


University of Calgary
Facutly of Medicine

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