[Histonet] Vibratome sectioning of mouse spinal cord

Michael, Susan michaels <@t> janelia.hhmi.org
Thu Nov 11 15:18:04 CST 2010

Can anyone give me some tips on sectioning mouse spinal cord on the vibratome?  Specifically, once the dura is successfully removed, I have been sectioning 40 um horizontal sections, and some cords have been curling up on themselves, both rostral-caudal and medial-lateral, and are impossible to uncurl without destroying the tissue.  I’ve tried every angle of approach, speed, amplitude, the tissue has been in sucrose, embedded in agarose, and chilled.



More information about the Histonet mailing list