[Histonet] Correcting van Gieson over-stain

Sean McBride smcbride <@t> andrew.cmu.edu
Wed Nov 10 15:36:34 CST 2010

Hi folks,

We have a set of slides that we stained in van Gieson's solution, and unfortunately, they turned out too dark.  I tried immersion in 100% ethanol for ~2 minutes, and that helped a little, but they are still too dark.  Does anyone have any experience or suggestions as to what solution to use to better differentiate the staining?  

Thanks in advance for all of your suggestions!


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