[Histonet] Processing rodent tissue

Wed Nov 10 13:36:50 CST 2010

I would just add that you do not need more than 2 hours total time in paraffin before embedding.

Date: Wed, 10 Nov 2010 10:16:43 -0500
From: Merced M Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] Paraffin Tissue Crumbles
To: Michael Mashore <mmashore <@t> vapop.ucsd.edu>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <449016B996B2D4CDC661E7FC <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=utf-8; format=flowed

Having some experience processing and embedding rodent tissues myself (by 
hand), I would say that you are over-dehydrating the tissues. Try cutting 
back the alcohol incubation times to 30 min or even 10 min each.

Regards,
Merced

--On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore 
<mmashore <@t> vapop.ucsd.edu> wrote:

> Hello Histonet Users,
>
>
>
> I have just started using paraffin and am having many difficulties. Most
> of the time my tissue crumbles when sectioning. I have no real experience
> in paraffin histology and have been given the task of becoming proficient
> by myself, so I am hoping for feedback as to why my tissue keeps
> crumbling. The tissue in question has been:  skeletal muscle, cardiac
> muscle, liver, and brain (all from rat).
>
>
>
> The tissue was fixed in 10% neutral buffered formalin for 7 days at 4Â°C
> and then transferred to an automated tissue processor, with the following
> schedule:
>
>
>
> 2 hours 70% dehydration alcohol
>
> 2 hours 80% dehydration alcohol
>
> 2 hours 95% dehydration alcohol
>
> 2.5 hours 95% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> .5 hour Hemo-De
>
> .5 hour Hemo-De
>
> .5 hour Hemo-De
>
> 1 hour paraffin
>
> 4 hours paraffin
>
>
>
> They were infiltrated for 1 hour without vacuum then embedded.
>
> The blocks were stored in the freezer before cutting.
>
> The knife angle was 5Â°.
>
> Sections were 5Âµm thick.
>
>
>
> I would appreciate any feedback whatsoever. 

John Ronan
Research Associate
Bioanalytics & Pathology
732 594-6378 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, November 10, 2010 12:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 84, Issue 11

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Today's Topics:

   1. RE: looking for references to stain protocols (Roberta Horner)
   2. Jaclyn Bhamornsiri - Important Message
      (Jaclyn_Bhamornsiri <@t> vwr.com)
   3. Histology Lab Survey (Lesley Bechtold)
   4. Cutting and embedding (Lin Bustamante)
   5. NSH Validation WS attendees (Patsy Ruegg)
   6. how to fix floating sections? (Caroline Bass)
   7. Part-time Lab opportunity in Scottsdale, AZ (Carlos Rodriguez, MD)
   8. Paraffin Tissue Crumbles (Michael Mashore)
   9. Histo data needed (O'Donnell, Bill)
  10. RE: Distance Learning (joelle weaver)
  11. Oil Red O trivia (Finlay Finlay)
  12. Re: Oil Red O trivia (Geoff McAuliffe)
  13. Oil Red O control (Inman, Anna)
  14. Sending Slides Out in St. Louis, MO (Alyssa Peterson)
  15. Re: Oil Red O control (Drew Meyer)
  16. RE: Oil Red O control (Sun,Yuping)
  17. Re: Paraffin Tissue Crumbles (Merced M Leiker)
  18. RE: Oil Red O control (Weems, Joyce)
  19. RE: Paraffin Tissue Crumbles (Nails, Felton)
  20. RE: Sending Slides Out in St. Louis, MO (Langenberg, Stacey)
  21. Position - Muscle Enzyme Histochem Tech (Barone, Carol )
  22. NH Histology Job Opportunity (Alisha Dynan)
  23. New York Dermpath Lab Manager (Alisha Dynan)
  24. Laboratory Manager position in MI (Alisha Dynan)
  25. Histology Supervisor Job in Oklahoma (Alisha Dynan)
  26. Histology Supervisor Position in GA (Alisha Dynan)

----------------------------------------------------------------------

Message: 1
Date: Tue, 9 Nov 2010 13:44:52 -0500
From: Roberta Horner <rjr6 <@t> psu.edu>
Subject: [Histonet] RE: looking for references to stain protocols
To: "Burton, Lynn" <Lynn.Burton <@t> Illinois.gov>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F12BB68C2BA80B4686A8EA261F80DD4599073991F5 <@t> pdc.padlspsu.psu.edu>
Content-Type: text/plain; charset="us-ascii"

This is the reference that I have for this stain.
Andersen A.A. & VanRompay D (2005). Chlamydiosis. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens, Fifth Edition. Submitted USA.

Roberta Horner HT/HTL
Animal Diagnostic La
Penn State University

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Burton, Lynn
Sent: Tuesday, November 09, 2010 12:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] looking for references to stain protocols

I am in need of references for stain protocols. The stain I am searching for in particular  is the modified Gimenez, also known as Pierce-Vanderkamp for chlamydia and rickettsia. If anyone can point me in the right direction I would appreciate it.

Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
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------------------------------

Message: 2
Date: Tue, 9 Nov 2010 14:00:43 -0500
From: Jaclyn_Bhamornsiri <@t> vwr.com
Subject: [Histonet] Jaclyn Bhamornsiri - Important Message
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OFD207FAAE.DAE0822E-ON852577D6.00686F8D-852577D6.00686F8D <@t> vwr.com>
Content-Type: text/plain; charset=US-ASCII

I will be out of the office starting  11/09/2010 and will not return until
12/31/2014.

As of Nov 9th, 2010, I am no longer with VWR. If you need assistance, pls
contact VWR Customer Service at 1800-932-5000 (or
vwrcustomerservice <@t> vwr.com). For more urgent matters, pls contact Leize
Lessig at 770-733-8190 (or leize_lessig <@t> vwr.com).

Thanks!
Jackie B.

------------------------------

Message: 3
Date: Tue, 9 Nov 2010 14:14:48 -0500
From: Lesley Bechtold <Lesley.Bechtold <@t> jax.org>
Subject: [Histonet] Histology Lab Survey
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<3BDA51FFD1A83B4E90829F594A5C371743316EECB8 <@t> JAXBHMAIL01.jax.org>
Content-Type: text/plain; charset="us-ascii"

Dear Histonetters,

Scientific Services at The Jackson Laboratory is conducting benchmarking surveys in an effort to develop an understanding of the current best practices in operations, management and technical delivery at peer institutions. The goal is to gather quality data that can be used to assist us and all survey participants with operational assessment and planning. Survey participants will receive compiled results which will be de-identified before distribution.

If you would like to participate, just click on the link below and answer the questions in the survey.  Additionally, for those of you who do EM, there is a link to an EM Facility survey as well.   It will only take a few minutes of your time.  With thanks in advance!

Lesley

Lesley S. Bechtold
Senior Manager, Histopathology Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322 (phone)
207-288-6325 (fax)

Histo Survey - http://www.surveymonkey.com/s/5RPSYG2

EM Survey - http://www.surveymonkey.com/s/5RGYH9W

------------------------------

Message: 4
Date: Tue, 09 Nov 2010 12:34:59 -0600
From: "Lin Bustamante" <lbustamante <@t> cvm.tamu.edu>
Subject: [Histonet] Cutting and embedding
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4CD93FF3020000B9000E58DC <@t> CVM.TAMU.EDU>
Content-Type: text/plain; charset=US-ASCII

Could you please tell me what is the average for embedding surgical
blocks in one hour?
Is there an average for cutting?
Thank you.
Lin.

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458

------------------------------

Message: 5
Date: Tue, 9 Nov 2010 13:29:37 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] NSH Validation WS attendees
To: "'ihcrg Group \(E-mail\)'" <ihcrg <@t> googlegroups.com>,
	<histonet <@t> lists.utsouthwestern.edu>,	"'National Society of
	Histotechnology'" <histo <@t> nsh.org>
Cc: "'Morken, Tim'" <Timothy.Morken <@t> ucsfmedctr.org>,
	Diane.Tokugawa <@t> kp.org
Message-ID: <78B71368E2844D3E9B0E2253896F8291 <@t> Patsyoffice>
Content-Type: text/plain;	charset="us-ascii"

For those of you who attended the IHC ws at NSH on Validation by Tim Morken
and Patsy Ruegg we have posted the rest of the information we promised you
to my ftp://ihctech.net <ftp://ihctech.net/>  site for you to download.  We
provided the user name and pass word you will need in the workshop.  The
folder is labeled "Tim Morken" and you can download the files to your
computer.  Remember this site is for uploading and downloading not opening.

Best regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email  <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site  <http://www.ihctech.net> www.ihctech.net

This email is confidential and intended solely for the use of the Person(s)
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------------------------------

Message: 6
Date: Tue, 9 Nov 2010 21:51:02 +0000
From: Caroline Bass <cbass <@t> wfubmc.edu>
Subject: [Histonet] how to fix floating sections?
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C8FF2C75.1344%cbass <@t> wfubmc.edu>
Content-Type: text/plain; charset="Windows-1252"

Hello Everyone,

I have a bunch of rat brains that I have frozen in isopentane and stored at -80. I'd like to collect 300 micron sections on a cryostat and collect tissue punches for RNA isolation. I would then like to take the remaining tissue and stain. There are a few ways of doing this, what would work the best is to take two 50 micron section, followed by a 300 micron, followed by two more 50, and go through the brain serially. I will then stain the 50 micron sections with nissl or thionin, so that the structures are easily identified and then use these to direct the tissue punches. The second 50 micron sections would be reserved for GFP IHC. Is this feasible? Here are some direct questions:

  1.  how would I store the 300 micron sections?
  2.  Any suggestions/protocols for staining the 50 micron sections for structure? Which stain would you use?
  3.  Can I post-fix the 50 micron sections? How about the 300 micron?

One reason I want to use floating sections for the nissl/thionin is that I can mount these very flat, no wrinkles and I'm very comfortable working with floating sections. I don't want to risk precious tissue trying to optimize this. One methodology recommended to me is to freeze a formalin solution in a 24 well plate, put the section on top flat and let them thaw together at room temperature or 4 degrees. This gives the tissue a nice, slow fixation.

Any other suggestions? How about working with the RNA? I'm thinking of using RNAlater-ice for example. Does anyone know if this screws up native fluorescence of GFP?

Thanks!

Caroline Bass

------------------------------

Message: 7
Date: Tue, 9 Nov 2010 15:47:21 -0700
From: "Carlos Rodriguez, MD" <cfrmd1 <@t> gmail.com>
Subject: [Histonet] Part-time Lab opportunity in Scottsdale, AZ
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTikaT=rR7TL1QZDkDCfT_-Pf9iN-Wg1j=W8NZDco <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi all.

We have a part-time job opportunity for someone interested primarily in
accessioning and grossing skin specimens at our in-house dermatopathology
lab (North Scottsdale Dermatology). A small amount of general lab management
will also be required (ie, maintaining CLIA logs, ordering supplies etc).
The hours needed will likely be Monday, Wednesday and Thursday mornings (~5
or 6am-9am). The start date will be around late December. The hours and
start date are flexible, and the workload is very reasonable.

Qualifications are either HT certification, or at a minimum an Associate's
Degree in a health-related field with college-level credit in Chemistry,
Biology, and Math, in addition to work experience in grossing and
accessioning skin specimens.

If interested, please respond to me by email and include a CV and/or
description of your qualifications.

Thanks!

Carlos Rodriguez, MD

------------------------------

Message: 8
Date: Tue, 9 Nov 2010 14:56:49 -0800
From: "Michael Mashore" <mmashore <@t> vapop.ucsd.edu>
Subject: [Histonet] Paraffin Tissue Crumbles
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <652586E049884D8491A0E70448595D40 <@t> vandeberg>
Content-Type: text/plain;	charset="iso-8859-1"

Hello Histonet Users,

I have just started using paraffin and am having many difficulties. Most of
the time my tissue crumbles when sectioning. I have no real experience in
paraffin histology and have been given the task of becoming proficient by
myself, so I am hoping for feedback as to why my tissue keeps crumbling. The
tissue in question has been:  skeletal muscle, cardiac muscle, liver, and
brain (all from rat).

The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C and
then transferred to an automated tissue processor, with the following
schedule:

2 hours 70% dehydration alcohol 

2 hours 80% dehydration alcohol

2 hours 95% dehydration alcohol

2.5 hours 95% dehydration alcohol

2 hours 100% dehydration alcohol

2 hours 100% dehydration alcohol

2 hours 100% dehydration alcohol

.5 hour Hemo-De

.5 hour Hemo-De

.5 hour Hemo-De

1 hour paraffin

4 hours paraffin

They were infiltrated for 1 hour without vacuum then embedded.

The blocks were stored in the freezer before cutting.

The knife angle was 5°. 

Sections were 5µm thick.

I would appreciate any feedback whatsoever. 

Thank you very much.

Michael

------------------------------

Message: 9
Date: Tue, 9 Nov 2010 16:39:27 -0700
From: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>
Subject: [Histonet] Histo data needed
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<E5BF2D5BFDCDFD49882F9DF0B9F1A609A1FC4E <@t> chimsx016.CHI.catholichealth.net>

Content-Type: text/plain; charset=us-ascii

Those who work in similar labs please help me out:

Could anyone tell me how a clinical histology lab that do 6500 cases a
year is staffed. (Average aboout 70 blocks a day, 20-30% small biopsies
that require 3 levels so about 120-140 "ribbons" a day) 
We do not do cyto processing, we have automated H&E.and coverslipping.
All special stains and immuno is does manually, but volume is low, 2-4
SS a week and maybe 6 antibodies.

We do gross probalbly 60-70% of cases and assist at gross for the rest.
We have no aides and are staffed @ 1.5 FTE. I'm trying to justify some
help, but the suits need numbers to crunch.

I hope that is enough  info for comparison.

Thanks in advance,
Bill 'the tired tech" O'Donnell

Kearrney, NE

------------------------------

Message: 10
Date: Wed, 10 Nov 2010 10:47:57 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] Distance Learning
To: <dchihc <@t> yahoo.com>, Histonet <histonet <@t> lists.utsouthwestern.edu>,
	<jweave01 <@t> cscc.edu>
Message-ID: <SNT135-w62044634E6908B279BFA94D8310 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Hi Phyllis
We offer an on line, distance learning program from Columbus State Community Colllege that is fully accredited by NAACLS. It awards a certificate in histotechnology and meets the training and eligibility requirements for the ASCP HT registry examination. The certificate program is linked to an Associates degree in the Department of Allied and Multi-competency Health for those wanting a degree option. We are currently accepting students for the next program year. Our application and admissions process and application form is available from our program web page at www.cscc.edu/histology 
We are looking to expand our market and meet the needs of students and laboratories outside of Ohio. We have a few clinical affiliates in the Midwest, other than Ohio, and we would like to extend this further into under-served areas, ( as far as histology schools). We are able to offer 24/7 access to materials, instruction in theory and practice, practical work assessment, and interactive delivery via Blackboard learning platform. In the future we plan to offer continuing education opportunities that are histology specific to those aready certified or who may participate in the ASCP- CMP program ,and want to meet the continuing education requirements needed for participation. 
If I may answer any questions about histology training, certification or the on line study of histology, please feel free to contact me at jweave01 <@t> cscc.edu or at 614-287-2217. 

 Joelle Weaver HTL (ASCP)

> Date: Mon, 8 Nov 2010 07:26:59 -0800
> From: dchihc <@t> yahoo.com
> To: Histonet <@t> lists.utsouthwestern.edu
> CC: 
> Subject: [Histonet] Distance Learning
> 
> We are thinking of using one of the online or distance learning programs to help 
> in the training of new histotechs. Has anyone had any experience with any of 
> these programs, what colleges offer the program, pros, cons. Any feedback is 
> welcome.
> 
> Thanks
>  Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL 
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 11
Date: Wed, 10 Nov 2010 14:24:32 -0000
From: "Finlay Finlay" <f.finlay <@t> formed.gla.ac.uk>
Subject: [Histonet] Oil Red O trivia
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<DED6E938EF692144990EBEFADEAEBB62894191 <@t> formed-ex.formed.internal>
Content-Type: text/plain;	charset="us-ascii"

Hello

Just wondering if anyone knows the what the 'O' in oil red O stands for.
I suspect that it stands for nothing other than O similar to the 'G' in
Orange G. One of our pathologists is looking for a tricky stain question
to annoy trainees with.

Thanks for your help

Finlay

------------------------------

Message: 12
Date: Wed, 10 Nov 2010 09:39:10 -0500
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Oil Red O trivia
To: Finlay Finlay <f.finlay <@t> formed.gla.ac.uk>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4CDAAE8E.20102 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

So, the pathologist does not know the answer but he wants to annoy 
trainees? Glad I don't work for him!

Geoff

Finlay Finlay wrote:
> Hello
>  
> Just wondering if anyone knows the what the 'O' in oil red O stands for.
> I suspect that it stands for nothing other than O similar to the 'G' in
> Orange G. One of our pathologists is looking for a tricky stain question
> to annoy trainees with.
>  
> Thanks for your help
>  
> Finlay
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************

------------------------------

Message: 13
Date: Wed, 10 Nov 2010 07:45:28 -0700
From: "Inman, Anna" <Anna.Inman <@t> stmarygj.org>
Subject: [Histonet] Oil Red O control
To: "Geoff McAuliffe" <mcauliff <@t> umdnj.edu>,	"Finlay Finlay"
	<f.finlay <@t> formed.gla.ac.uk>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<2925AE271EAAD440AF48FCCEB8002D091443E583 <@t> smgmail01.smgj.sclhs.net>
Content-Type: text/plain; charset="us-ascii"

Hello -

How does everyone handle having a positive control for Oil Red O - We
very infrequently have this stain and have had difficulty keeping
control on hand. 

Thank you 

Anna

CONFIDENTIALITY NOTICE:  This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.

------------------------------

Message: 14
Date: Wed, 10 Nov 2010 09:51:19 -0500
From: Alyssa Peterson <alyssa <@t> alliedsearchpartners.com>
Subject: [Histonet] Sending Slides Out in St. Louis, MO
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTinyJNcfvsgQtm0o0rNf+E4rFg7Lc7o3aA6_TRsi <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hello,

A friend of mine who has a brand new laboratory in St. Louis, MO currently
has their histotech prepare their slides but are looking for a laboratory to
have them read at because they do not have a Dermatopathologist. Please
contact me if you have  lab in the area who would be interested in doing
that for them. Thank you!

-- 
Alyssa Peterson, Director of Candidate Recruitment
LinkedIN:http://www.linkedin.com/in/alyssapetersonasp

Allied Search Partners

T: 888.388.7571

F: 888.388.7572

www.alliedsearchpartners.com

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or its attachments is prohibited.  If you have received this message in
error, please notify us immediately, and delete this message and its
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------------------------------

Message: 15
Date: Wed, 10 Nov 2010 09:54:46 -0500
From: Drew Meyer <41dmb41 <@t> gmail.com>
Subject: Re: [Histonet] Oil Red O control
To: "Inman, Anna" <Anna.Inman <@t> stmarygj.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTimKNCBrB-ix-62azG2eJDPOtqUO88yFtJHEe+NO <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

There was a discussion on this recently, so you may want to search the
archives for it.  In my opinion, the easiest and best control to use is
Mayo.  Just smear it on a slide as you need it and there you go.  Cheap and
easy.

Drew

On Wed, Nov 10, 2010 at 09:45, Inman, Anna <Anna.Inman <@t> stmarygj.org> wrote:

> Hello -
>
> How does everyone handle having a positive control for Oil Red O - We
> very infrequently have this stain and have had difficulty keeping
> control on hand.
>
>
>
>
>
> Thank you
>
> Anna
>
> CONFIDENTIALITY NOTICE:  This e-mail message, including any attachments, is
> for the sole use of the intended recipient(s) and may contain confidential
> and privileged information. Any unauthorized review, use, disclosure or
> distribution is prohibited. If you are not the intended recipient, please
> contact the sender by reply e-mail and destroy all copies of the original
> message.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

------------------------------

Message: 16
Date: Wed, 10 Nov 2010 10:11:41 -0500
From: "Sun,Yuping" <sun <@t> pathology.ufl.edu>
Subject: RE: [Histonet] Oil Red O control
To: "'Drew Meyer'" <41dmb41 <@t> gmail.com>, "'Inman, Anna'"
	<Anna.Inman <@t> stmarygj.org>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<530A57D46C74F34B862F95E3F4DFF3228D5D2722 <@t> HSC-CMS01.ad.ufl.edu>
Content-Type: text/plain; charset="us-ascii"

Hello Histonet Users,

I have a question about Oil Red O staining.
Could the Oil Red O solution(Poly Scientific, Cat.No. S1848) be reused?

I would appreciate any feedback whatsoever. 

Thank you very much.
Cathy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Drew Meyer
Sent: Wednesday, November 10, 2010 9:55 AM
To: Inman, Anna
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil Red O control

There was a discussion on this recently, so you may want to search the
archives for it.  In my opinion, the easiest and best control to use is
Mayo.  Just smear it on a slide as you need it and there you go.  Cheap and
easy.

Drew

On Wed, Nov 10, 2010 at 09:45, Inman, Anna <Anna.Inman <@t> stmarygj.org> wrote:

> Hello -
>
> How does everyone handle having a positive control for Oil Red O - We
> very infrequently have this stain and have had difficulty keeping
> control on hand.
>
>
>
>
>
> Thank you
>
> Anna
>
> CONFIDENTIALITY NOTICE:  This e-mail message, including any attachments, is
> for the sole use of the intended recipient(s) and may contain confidential
> and privileged information. Any unauthorized review, use, disclosure or
> distribution is prohibited. If you are not the intended recipient, please
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> message.
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_______________________________________________
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------------------------------

Message: 17
Date: Wed, 10 Nov 2010 10:16:43 -0500
From: Merced M Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] Paraffin Tissue Crumbles
To: Michael Mashore <mmashore <@t> vapop.ucsd.edu>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <449016B996B2D4CDC661E7FC <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=utf-8; format=flowed

Having some experience processing and embedding rodent tissues myself (by 
hand), I would say that you are over-dehydrating the tissues. Try cutting 
back the alcohol incubation times to 30 min or even 10 min each.

Regards,
Merced

--On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore 
<mmashore <@t> vapop.ucsd.edu> wrote:

> Hello Histonet Users,
>
>
>
> I have just started using paraffin and am having many difficulties. Most
> of the time my tissue crumbles when sectioning. I have no real experience
> in paraffin histology and have been given the task of becoming proficient
> by myself, so I am hoping for feedback as to why my tissue keeps
> crumbling. The tissue in question has been:  skeletal muscle, cardiac
> muscle, liver, and brain (all from rat).
>
>
>
> The tissue was fixed in 10% neutral buffered formalin for 7 days at 4Â°C
> and then transferred to an automated tissue processor, with the following
> schedule:
>
>
>
> 2 hours 70% dehydration alcohol
>
> 2 hours 80% dehydration alcohol
>
> 2 hours 95% dehydration alcohol
>
> 2.5 hours 95% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> .5 hour Hemo-De
>
> .5 hour Hemo-De
>
> .5 hour Hemo-De
>
> 1 hour paraffin
>
> 4 hours paraffin
>
>
>
> They were infiltrated for 1 hour without vacuum then embedded.
>
> The blocks were stored in the freezer before cutting.
>
> The knife angle was 5Â°.
>
> Sections were 5Âµm thick.
>
>
>
> I would appreciate any feedback whatsoever.
>
>
>
> Thank you very much.
>
>
>
> Michael
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.

------------------------------

Message: 18
Date: Wed, 10 Nov 2010 10:20:27 -0500
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Oil Red O control
To: "Inman, Anna" <Anna.Inman <@t> stmarygj.org>, Geoff McAuliffe
	<mcauliff <@t> umdnj.edu>, Finlay Finlay <f.finlay <@t> formed.gla.ac.uk>
Cc: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<92AD9B20A6C38C4587A9FEBE3A30E164044A071B7A <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

Smear mayonnaise on a slide.. It works well. And of course - not the fat free kind!  j 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Inman, Anna
Sent: Wednesday, November 10, 2010 09:45
To: Geoff McAuliffe; Finlay Finlay
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Oil Red O control

Hello -

How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. 

Thank you 

Anna

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------------------------------

Message: 19
Date: Wed, 10 Nov 2010 09:25:47 -0600
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Subject: RE: [Histonet] Paraffin Tissue Crumbles
To: "'Merced M Leiker'" <leiker <@t> buffalo.edu>,	"Michael Mashore"
	<mmashore <@t> vapop.ucsd.edu>,	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<C1FE5960057C084CA389CE97779062908C70BD9F <@t> TCDMSG01.ad.TexasChildrensHospital.org>

Content-Type: text/plain; charset=iso-8859-1

I agree most of your times are too long, but you can still get sections if you put water on your ice block and allow the tissue to rehydrate a bit or put a little ammonium hydroxide and water on your ice and sit your blocks to be section in this solution. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M Leiker
Sent: Wednesday, November 10, 2010 9:17 AM
To: Michael Mashore; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Tissue Crumbles

Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each.

Regards,
Merced

--On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore <mmashore <@t> vapop.ucsd.edu> wrote:

> Hello Histonet Users,
>
>
>
> I have just started using paraffin and am having many difficulties. 
> Most of the time my tissue crumbles when sectioning. I have no real 
> experience in paraffin histology and have been given the task of 
> becoming proficient by myself, so I am hoping for feedback as to why 
> my tissue keeps crumbling. The tissue in question has been:  skeletal 
> muscle, cardiac muscle, liver, and brain (all from rat).
>
>
>
> The tissue was fixed in 10% neutral buffered formalin for 7 days at 
> 4°C and then transferred to an automated tissue processor, with the 
> following
> schedule:
>
>
>
> 2 hours 70% dehydration alcohol
>
> 2 hours 80% dehydration alcohol
>
> 2 hours 95% dehydration alcohol
>
> 2.5 hours 95% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> 2 hours 100% dehydration alcohol
>
> .5 hour Hemo-De
>
> .5 hour Hemo-De
>
> .5 hour Hemo-De
>
> 1 hour paraffin
>
> 4 hours paraffin
>
>
>
> They were infiltrated for 1 hour without vacuum then embedded.
>
> The blocks were stored in the freezer before cutting.
>
> The knife angle was 5°.
>
> Sections were 5µm thick.
>
>
>
> I would appreciate any feedback whatsoever.
>
>
>
> Thank you very much.
>
>
>
> Michael
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Message: 20
Date: Wed, 10 Nov 2010 08:30:22 -0700
From: "Langenberg, Stacey" <STACEY.LANGENBERG <@t> UCDENVER.EDU>
Subject: RE: [Histonet] Sending Slides Out in St. Louis, MO
To: Alyssa Peterson <alyssa <@t> alliedsearchpartners.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1F70FCBB6D4EC549B2ADF69B9F9EAC0348F696A29E <@t> STEAMBOAT.ucdenver.pvt>
Content-Type: text/plain; charset="us-ascii"

Hi Alyssa,
We are not in St Louis but I work for a really great Dermpath here in Colorado who might read them for you.

"People are not an interruption of our business. People are our business."

Stacey Langenberg HT (ASCP) QIHC
Laboratory Manager
Histology/IF
CU Dermatopathology Consultants
1999 N. Fitzsimons Pkwy Suite 120
Aurora, CO 80045
Lab-720-859-3559  Fax- 303-344-0789  Office- 303-577-2303 Cell-970-405-7742
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson [alyssa <@t> alliedsearchpartners.com]
Sent: Wednesday, November 10, 2010 7:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sending Slides Out in St. Louis, MO

Hello,

A friend of mine who has a brand new laboratory in St. Louis, MO currently
has their histotech prepare their slides but are looking for a laboratory to
have them read at because they do not have a Dermatopathologist. Please
contact me if you have  lab in the area who would be interested in doing
that for them. Thank you!

--
Alyssa Peterson, Director of Candidate Recruitment
LinkedIN:http://www.linkedin.com/in/alyssapetersonasp

Allied Search Partners

T: 888.388.7571

F: 888.388.7572

www.alliedsearchpartners.com

This email including its attachments is intended only for the confidential
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------------------------------

Message: 21
Date: Wed, 10 Nov 2010 11:00:57 -0500
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] Position - Muscle Enzyme Histochem Tech
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<37E4BAC017F57141AF64FAA5AEB04CE8033A81A3 <@t> wlmmsx01.nemours.org>
Content-Type: text/plain;	charset="us-ascii"

Hello Histonetters: We presently have a position available for a HT/HTL
(certified, or eligible) in our clinical muscle enzyme histochemistry
lab (within the Department of Biomedical Research). We request 3 yrs
experience minimum in EHC... and skills for manual IF, as well. If you
meet these requirements and have interest, please contact
cbarone <@t> nemours.org. We are looking for you!

------------------------------

Message: 22
Date: 10 Nov 2010 11:55:24 -0500
From: Alisha Dynan <alisha <@t> ka-recruiting.com>
Subject: [Histonet] NH Histology Job Opportunity
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <607630384.1289408124244.JavaMail.cfservice <@t> SL4APP4>
Content-Type: text/plain; charset="utf-8"

Hi Histonet Members,

I am currently working on a Histology Supervisor and Histology Technologist job opportunity in Southern NH. These job opportunities are with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salray and benefits.