[Histonet] Frozen section ...HELP

[louise renton]

Hi all,

after more than a decade of NOT cutting frozen sections, I find myself
back at the ice-face.
To get my hand in (not literally) I thought I would do some trial
sections on stored tissue - stuff that was in formalin and now in 70%
alcohol.

Horror ; dismay. The tissue, once frozen, is all mushy in the middle.
Is this because of teh long storage?

is there anything I can do to improve the situation?

much appreciated

-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
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