[Histonet] Paraffin liver sections
Adam .
anonwums1 <@t> gmail.com
Thu Nov 4 22:34:21 CDT 2010
Thanks to all the prompt responses. He fixed the entire liver in excess
formalin (I think around 15 mL) with rocking for 3 days. I'm not sure about
the processing schedule; I'll have to contact the histology core to ask.
I forwarded all of the advice along, and he tried soaking the blocks in ice
water for several minutes. It fixed most of the problem, at least for now.
You people are far too helpful.
Thanks,
Adam
On Thu, Nov 4, 2010 at 2:53 PM, Jay Lundgren <jaylundgren <@t> gmail.com> wrote:
> Sounds to me like they are overprocessed (dried out). Try soaking them on
> ice for 30 minutes or so before cutting. Some people use a drop of fabric
> softener on their ice tray to soften the tissue, or there are commercial
> products like soft block from Polyscience.
>
> Jay A. Lundgren M.S.,
> HTL (ASCP)
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