[Histonet] Paraffin liver sections

[Pamela Marcum]

What is your processing schedule for the livers?  It is hard to know without that information. 



Pam Marcum 

UAMS 





----- Original Message ----- 
From: "Adam ." <anonwums1 <@t> gmail.com> 
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, November 4, 2010 1:08:03 PM 
Subject: [Histonet] Paraffin liver sections 

Dear all, 

One of my colleagues has consulted me about some paraffin embedded mouse 
livers he's been trying to cut. They were fixed in neutral buffered formalin 
for 3 days and then processed and embedded into paraffin. He was trying to 
cut 5 uM sections on our microtome, except as soon as the blade hit the 
liver, the liver would start to roll up and collapse into a small sliver, 
and you'd just get sections of paraffin with a hole where the liver should 
be. We tried changing cutting angle and soaking the blocks in ice water with 
no success. This is the first time we've tried livers, but we routinely cut 
decalcified bones on that microtome and have never seen that. Any ideas on 
what is going on? 

Thanks, 
Adam 
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Histonet mailing list 
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 






----- Original Message ----- 
From: "Adam ." <anonwums1 <@t> gmail.com> 
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, November 4, 2010 1:08:03 PM 
Subject: [Histonet] Paraffin liver sections 

Dear all, 

One of my colleagues has consulted me about some paraffin embedded mouse 
livers he's been trying to cut. They were fixed in neutral buffered formalin 
for 3 days and then processed and embedded into paraffin. He was trying to 
cut 5 uM sections on our microtome, except as soon as the blade hit the 
liver, the liver would start to roll up and collapse into a small sliver, 
and you'd just get sections of paraffin with a hole where the liver should 
be. We tried changing cutting angle and soaking the blocks in ice water with 
no success. This is the first time we've tried livers, but we routinely cut 
decalcified bones on that microtome and have never seen that. Any ideas on 
what is going on? 

Thanks, 
Adam 
_______________________________________________ 
Histonet mailing list 
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet