[Histonet] RE: Higgins Ink

[Truscott, Tom]

Hi Allison, Trying to think why it washed off, I wondered if the skin went back into formalin after the acetic acid, or did it go to alcohol, perhaps setting up an acid alcohol condition that maybe was enough to decolorize it. Tom T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Tuesday, May 25, 2010 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Higgins Ink

Hello to all in histo land.  Our PA used Higgins ink (india ink) on some
skin specimens and set the ink with 10%acetic acid soln.  After the
specimens were processed, all of the ink was gone, washed off.  We have
regular alcohols and xylene on the processor, this should not have made
the ink wash off.  Any suggestions on why it washed off, how to prevent
this from happening again.  Any help in this matter will be greatly
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet