[Histonet] RE: Higgins Ink

Truscott, Tom ttruscot <@t> vetmed.wsu.edu
Wed May 26 09:40:32 CDT 2010


Hi Allison, Trying to think why it washed off, I wondered if the skin went back into formalin after the acetic acid, or did it go to alcohol, perhaps setting up an acid alcohol condition that maybe was enough to decolorize it. Tom T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Tuesday, May 25, 2010 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Higgins Ink

Hello to all in histo land.  Our PA used Higgins ink (india ink) on some
skin specimens and set the ink with 10%acetic acid soln.  After the
specimens were processed, all of the ink was gone, washed off.  We have
regular alcohols and xylene on the processor, this should not have made
the ink wash off.  Any suggestions on why it washed off, how to prevent
this from happening again.  Any help in this matter will be greatly
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
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