[Histonet] RE: Higgins Ink

Tom McNemar TMcNemar <@t> lmhealth.org
Wed May 26 05:08:25 CDT 2010


We use Higgins as well.  For many years we have set it with nothing more than regular white vinegar.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Tuesday, May 25, 2010 4:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Higgins Ink

Hello to all in histo land.  Our PA used Higgins ink (india ink) on some
skin specimens and set the ink with 10%acetic acid soln.  After the
specimens were processed, all of the ink was gone, washed off.  We have
regular alcohols and xylene on the processor, this should not have made
the ink wash off.  Any suggestions on why it washed off, how to prevent
this from happening again.  Any help in this matter will be greatly
appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
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