[Histonet] Problems w/IHC's

[Karla Arrington]

Hello Histo's:

We are having troubles with getting our Cyclin D1 to stain for longer than one month. I seems to whimp out after a month, so we make up fresh.  Any ideas? We are using a Dako clone. Also we are currently working up Surfactant A with little luck.  Our lung control does not stain at all while our patient panel is weak. We are doing 1:00 dilution for 30 mts and 30 mts using high pH (9.9) for retrieval. Any help would be greatly appreciated!!

Karla Arrington
HT(ASCP)