[Histonet] Yellow counterstain

[Bryan Hewlett]

Mike,

There are a number of yellow counterstains commonly used, including picric 
acid.
For your purpose, I would recommend the following in order of preference;

Tartrazine (Acid yellow 23, Food yellow 4) CI 19140,
Metanil yellow (Acid yellow 31) CI 13065,
Martius yellow (Acid yellow 24) CI 10315.

Regards,

Bryan

----- Original Message ----- 
From: "Mike Tighe" <mtighe <@t> trudeauinstitute.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, May 24, 2010 12:13 PM
Subject: [Histonet] Yellow counterstain



I have seen Tissue sections counterstained with a yellow counterstain. I 
would like to use an Iron hematoxylin with this counter stain. Can anyone 
tell me what this is? Are there any tips/tricks to this counterstain? Thanks 
in advance!

Mike


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