[Histonet] afb contamination

[Jackie M O'Connor]

You can eliminate the crossover contamination by performing the stain 
horizontally on a staining rack.  A lot of labs re-use the carbol fuchsin, 
which can have lots of extraneous AFB floating around, waiting patiently 
to attach itself to an unsuspecting patient slide.  Using the staining 
rack, you don't use a lot of reagent, and you never cross-contaminate.




From:
"Tench, Bill" <Bill.Tench <@t> pph.org>
To:
histonet <@t> lists.utsouthwestern.edu
Date:
05/21/2010 02:34 PM
Subject:
[Histonet] afb contamination
Sent by:
histonet-bounces <@t> lists.utsouthwestern.edu



We had a problem with contamination on our AFB stains, and we discovered
that it was the control slide flaking off in the copland jar which was
being used for staining the control and target slide at the same time
(makes sense as a real "control').  We identified these contaminants
because they were frequently large clusters (by "large" I would say 4-8
organisms) which we almost never see in real cases, and fortunately,
they were also not in the same plane of focus (but that can be subtle).
they did create problems.  Our solution was to stain the control
separately from the case.  No more problems. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

Bill.Tench <@t> pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


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