[Histonet] RE: possible AFB contaminant

[Della Speranza, Vinnie]

Do you use DI water in your flotation baths ?

If your DI system is not already so equipped, install a 0.5 micron filter where you withdraw the water and be sure to change this filter monthly.

I'm willing to bet the bugs are on the slides before the stain is performed 


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Debra.Ortiz <@t> uchospitals.edu
Sent: Thursday, May 20, 2010 3:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] possible AFB contaminant

Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are "false positives" on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths.
Any advice or comments would be greatly appreciated.
 
Thanks Debi

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Thank you
University of Chicago Medical Center 
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