[Histonet] Nissl Stain P0 Mouse Head

[Kevin Gillinder]

Hi,

I am having some trouble with Nissl staining newborn (P0) mouse heads/hindbrain.
The nissl stain appears very faint in the centre of my sections. (Paraffin embedded, 7um thick)

Is this likely to be a penetration issue?
I fixed in 4% PFA  O/N before processing - Would that affect it?
I left the skull intact, but removed the skin - should I have removed some of the skull?

Any help appreciated!!!!!!

Regards,

--
Kevin