[Histonet] weekend fixation

Mike Pence mpence <@t> grhs.net
Thu May 20 16:28:51 CDT 2010


Yeah. Good luck with that one. The minimum clearly states 6 hrs.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> xbiotech.com
Sent: Thursday, May 20, 2010 3:08 PM
To: LINDA MARGRAF
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] weekend fixation



   From  what I have seen and heard you can have fixation times up to=2
   hours and still be ok?

   Sarah Goebel, B.A., HT (ASCP)

   <=iv>

   Histo=echnician
   
   XBiotech USA Inc.

   8201 East Rivers=de Dr. Bldg 4 Suite 100

   Austin, Texas  78744
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] weekend fixation
   From: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>
   Date: Thu, May 20, 2010 12:04 pm
   To: <histonet <@t> lists.utsouthwestern.edu>
   Histonetters:
   Here's a message I was asked to post......
   Dear Colleagues,
   I  have  the  following question concerning tissue processing. We do
a
   lot  o= IHC work on NF fixed tissue. To standardize and minimize the
   effect  of  NF=ixation, we fixate the tissue always for 24h. This is
   of  course  a  problem=for  tissues taken on Friday. In the past, we
   asked  our  technicians  to  come=n Saturday to embed the tissues in
   paraffin.  Unfortunately,  this is not p=ssible anymore, and that is
   why  I  need  your  advice.  What would you suggest= 1) to leave the
   tissue  in  NF  until  Sunday evening and start processing, =r 2) to
   keep  the  fixation  time (24 hours) and leave the tissue in alcohol
70% until Sunday evening and then start processing.
   Thanks for your advice.
   Kind regards,
   Wim.
   Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
   Cell biology and Histology
   Department of Morphology - Faculty of Veterinary Medicine
   Ghent University
   Salisburylaan 133, B-9820 Merelbeke, BELGIUM
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