[Histonet] weekend fixation

[Mike Pence]

Yeah. Good luck with that one. The minimum clearly states 6 hrs.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> xbiotech.com
Sent: Thursday, May 20, 2010 3:08 PM
To: LINDA MARGRAF
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] weekend fixation



   From  what I have seen and heard you can have fixation times up to=2
   hours and still be ok?

   Sarah Goebel, B.A., HT (ASCP)

   <=iv>

   Histo=echnician
   
   XBiotech USA Inc.

   8201 East Rivers=de Dr. Bldg 4 Suite 100

   Austin, Texas  78744
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] weekend fixation
   From: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>
   Date: Thu, May 20, 2010 12:04 pm
   To: <histonet <@t> lists.utsouthwestern.edu>
   Histonetters:
   Here's a message I was asked to post......
   Dear Colleagues,
   I  have  the  following question concerning tissue processing. We do
a
   lot  o= IHC work on NF fixed tissue. To standardize and minimize the
   effect  of  NF=ixation, we fixate the tissue always for 24h. This is
   of  course  a  problem=for  tissues taken on Friday. In the past, we
   asked  our  technicians  to  come=n Saturday to embed the tissues in
   paraffin.  Unfortunately,  this is not p=ssible anymore, and that is
   why  I  need  your  advice.  What would you suggest= 1) to leave the
   tissue  in  NF  until  Sunday evening and start processing, =r 2) to
   keep  the  fixation  time (24 hours) and leave the tissue in alcohol
70% until Sunday evening and then start processing.
   Thanks for your advice.
   Kind regards,
   Wim.
   Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
   Cell biology and Histology
   Department of Morphology - Faculty of Veterinary Medicine
   Ghent University
   Salisburylaan 133, B-9820 Merelbeke, BELGIUM
   Please consider the environment before printing this e-mail.
   This  e-mail,  facsimile,  or  letter  and  any  files  or
attachments
   transmitted =ith it contains
   information  that  is confidential and privileged. This information
is
   intend=d only for the
   use  of  the individual(s) and entity(ies) to whom it is addressed.
If
   you ar= the intended
   recipient,   further   disclosures   are   prohibited  without
proper
   authorization.=f you are not
   the  intended  recipient, any disclosure, copying, printing, or use
of
   this i=formation is
   strictly  prohibited  and possibly a violation of federal or state
law
   and re=ulations. If you
   have  received  this  information  in  error, please notify
Children's
   Medical C=nter Dallas
   immediately  at  214-456-4444  or via e-mail at
privacy <@t> childrens.com.
   Childre='s Medical
   Center   Dallas   and  its  affiliates  hereby  claim  all
applicable
   privileges rel=ted to this
   information.
   _______________________________________________
   Histonet mailing list
   Histonet <@t> lists.utsouthwestern.edu
   [1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<=r>

References

   1.
3D"http://lists.utsouthwestern.edu/mailman/listin_______________________
________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet