[Histonet] weekend fixation

[Liz Chlipala]

I found the reference

Goldstein NS, Ferkowicz M, Odish E, et al: Minimum formalin fixation
time for consistent estrogen receptor immunohistochemical staining of
invasive breast carcinoma Am J Clin Pathol 120:86-92, 2003

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LINDA
MARGRAF
Sent: Thursday, May 20, 2010 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] weekend fixation


 Histonetters:
Here's a message I was asked to post......

Dear Colleagues,
 I  have the following question concerning tissue processing. We do a
lot of IHC work on NF fixed tissue. To standardize and minimize the
effect of NF fixation, we fixate the tissue always for 24h. This is of
course a problem for tissues taken on Friday. In the past, we asked our
technicians to come on Saturday to embed the tissues in paraffin.
Unfortunately, this is not possible anymore, and that is why I need your
advice. What would you suggest ? 1) to leave the tissue in NF until
Sunday evening and start processing, or 2) to keep the fixation time (24
hours) and leave the tissue in alcohol 70% until Sunday evening and then
start processing.
 Thanks for your advice.
Kind regards,
Wim.
 

Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM



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