[Histonet] unsubscribe
Aidan Schurr
Aidan.Schurr <@t> leica-microsystems.com
Thu May 20 01:15:24 CDT 2010
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Subject: Histonet Digest, Vol 78, Issue 28
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Today's Topics:
1. RE: Freezing spray artifact (Shirley A. Powell)
2. RE: IHC Validation on new instrument (Liz Chlipala)
3. Re: Freezing spray artifact (Rena Fail)
4. RE: Freezing spray artifact (Sebree Linda A)
5. IHC Validation on microwaved tissue (Matt Brooks)
6. charging for cytospins (Tench, Bill)
7. Re: Re: Grossing Technician Qualifications (Daniel Schneider)
8. Temp Histo Tech Needed (Marcia Fisher)
9. IHC Validation on new instrument (Troutman, Kenneth A)
10. PPE (Cindy DeRiso)
11. RE: Freezing spray artifact (sgoebel <@t> xbiotech.com)
12. RE: Re: Grossing Technician Qualifications (Mahoney,Janice A)
13. RE: PPE (Liz Chlipala)
14. RE: PPE (Sherwood, Margaret )
15. Re: Re: Grossing Technician Qualifications (Daniel Schneider)
16. IHC Validation (again) (Laurie Colbert)
17. Re: alternative for bunsen burner? (Debbie Dreesen)
18. Thomas Crowell is out of the office. (thomas.crowell <@t> novartis.com)
19. RE: IHC Validation (again) (Liz Chlipala)
20. Re: Freezing spray artifact (James L Burchette)
21. Equipment Repair Dude (sgoebel <@t> xbiotech.com)
22. AANLkTik8dnsQt16RPMSS8_x5fgqQE_18-ctUeNp71V0L <@t> mail.gmail.com
(Andrew Burgeson)
23. Histonet (Rebecca Johnson)
24. bone processing (Andrew Burgeson)
25. Re: Grossing Technician Qualifications (Robert Richmond)
26. RE: Freezing spray artifact (Jennifer MacDonald)
----------------------------------------------------------------------
Message: 1
Date: Wed, 19 May 2010 13:15:37 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: [Histonet] RE: Freezing spray artifact
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE2268C5518AA <@t> MERCERMAIL.MercerU.local>
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There is a text written by the late Lee Luna and Samuel Wesley Thompson entitled An Atlas of Artifacts in this the artifact from freeze spray is pictured. The ISBN # is 0-398-03624-1 Published by Charles C. Thomas, Publisher.
Shirley Powell
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Wednesday, May 19, 2010 12:09 PM
To: histonet
Subject: [Histonet] Freezing spray artifact
Has anyone run into a problem or artifact from freezing spray? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.
Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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------------------------------
Message: 2
Date: Wed, 19 May 2010 11:21:32 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] IHC Validation on new instrument
To: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B101134 <@t> server.PremierLab.local>
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We perform our entire validation process as a new piece of equipment.
Our validation protocols are quite extensive, up to about 85 pages long on each piece of major equipment, at least that's what it was for our new prisma stainer and glass coverslipper. We perform an installation/operational qualification protocol or an IOQ. If we move the instrument we also do the same thing, we already have the protocol written which takes most of the time we just execute it again. We are a GLP lab so we work off a Validation Master Plan that basically tells us how we are going to validate each piece of equipment in the lab.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Wednesday, May 19, 2010 9:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation on new instrument
What do others do when validating a new model of a piece of equipment - same manufacturer, same basic staining process, but an updated version of the equipment?
I've been told the protocols should be the same and that we only need to run three controls with three different but similar protocols to determine what looks best. Do you all think that is thorough enough, or would you run actual patient cases and compare old and new equipment? I don't see where the CAP checklist refers to new equipment - just new antibodies and new antibody lots.
Laurie Colbert
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------------------------------
Message: 3
Date: Wed, 19 May 2010 10:30:49 -0700 (PDT)
From: Rena Fail <renafail <@t> bellsouth.net>
Subject: Re: [Histonet] Freezing spray artifact
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <96641.83001.qm <@t> web180301.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Erin,
Holding the can too close and/or spraying for a prolonged period both cause freeze artifact. Look at your block, it will not have a smooth look to the paraffin. it will have lines it much like a cracked piece of glass. the tissue will appear cracked on the waterbath and will even separate along these lines. The cracks can be seen microscopically amd interfere with the architecture of the tissue.
Rena Fail
----- Original Message ----
From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Wed, May 19, 2010 12:09:22 PM
Subject: [Histonet] Freezing spray artifact
Has anyone run into a problem or artifact from freezing spray? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.
Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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------------------------------
Message: 4
Date: Wed, 19 May 2010 12:38:32 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Freezing spray artifact
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>, "histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8C023B4AB999614BA4791BAEB26E2738399E63 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
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Erin,
One can certainly get cracking of the tissue and surrounding paraffin from a too intense application of freezing spray. This is usually grossly visible, at least in the paraffin.
Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Wednesday, May 19, 2010 11:09 AM
To: histonet
Subject: [Histonet] Freezing spray artifact
Has anyone run into a problem or artifact from freezing spray? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like.
Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Wed, 19 May 2010 10:44:40 -0700
From: "Matt Brooks" <mbrooks <@t> incytepathology.com>
Subject: [Histonet] IHC Validation on microwaved tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<706224670091FE47997AEF88EFADE7CA01550C9A <@t> EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>
Content-Type: text/plain; charset="us-ascii"
Hello All,
We have new microwave tissue processor, until this purchase all tissue has been placed on a standard VIP processor. My medical director would like for most of our 125 antibodies worked up on microwave processed tissue. I have been challenged with getting specimens to process on the instrument for IHC antibody validation purposes. Initially we will be processing GI biopsy specimens, but we will eventually progress to other biopsy tissues and so on. I have had success getting some colon cancer cases, some tonsil and other tissue. We will be mainly performing H.
pylori, which is hard to get a large enough specimen to obtain a sample for this purpose. Anyway I was wondering how other labs are handling validation in this situation. Thank you for your time in this matter.
Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks <@t> incytepathology.com
509-892-2744
------------------------------
Message: 6
Date: Wed, 19 May 2010 10:52:21 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] charging for cytospins
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863103 <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii
Can you charge for two different stains for the urine cytospin?
The answer to this question is a "depends." If you are just doing any of the "optional" stains that may be used on a cytology preparation (namely Pap, H&E, romanovsky) you are NOT permitted to charge for each of these (they are not considered "special stains"). If you did an iron stain, or melanin stain, or mucin stain, you could charge separately for these "special" stains.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
mail2.pph.org made the following annotations
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Message: 7
Date: Wed, 19 May 2010 12:57:55 -0500
From: Daniel Schneider <dlschneider <@t> gmail.com>
Subject: Re: [Histonet] Re: Grossing Technician Qualifications
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AANLkTilwnx44kDOoCv4wzPcvG-q-iPvRlEt76IHJcoeL <@t> mail.gmail.com>
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Just to clarify or perhaps cloud the picture a little more
Not all grossing is "grossing."
So when we're talking about transferring small biopsies, in their entirety, from a formalin container to a cassette, and describing the size, number, and color of the tissue pieces submitted, with no dissection or knowledge of anatomy required, that's not "grossing" in the strict sense, at least according to CAP the last time I looked, which admittedly was a couple of years ago.
So what sort of educational achievements, training, or credentials are required for the above?
Dan Schneider
------------------------------
Message: 8
Date: Wed, 19 May 2010 10:57:39 -0700
From: "Marcia Fisher" <mfisher <@t> ecrmc.org>
Subject: [Histonet] Temp Histo Tech Needed
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3ACBB5D73A417547A01970CD3EB55093047B626B <@t> MAIL1.ecrmc.ci.el-centro.ca.us>
Content-Type: text/plain; charset="iso-8859-1"
We are in need of a temporary, ASCP certified histotech for 4-6 weeks beginning June 21 at El Centro Regional Medical Center. Located just 2 hours east of San Diego, 100 miles south of Palm Springs and only 60 miles west of Yuma. Our new lab was just completed in February, 2010 and is state-of-the-art.
El Centro Regional Medical Center is a nonprofit, community based hospital owned by the City of El Centro. For over 50 years, ECRMC has provided medical care to the Imperial Valley and has become a leading healthcare system as an excellent source for health care services.
In addition to the medical center, the community is served by two Outpatient Centers, a Wound Healing Center, a Children's Specialist Clinic and various educational programs including Asthma and Diabetes.
With more than 150 accredited physicians and over 900 employees, El Centro Regional Medical Center strives for excellence in the Imperial Valley.
Please contact me thru work email if interested and more details. mfisher <@t> ecrmc.org
M. Fisher
El Centro Regional Medical Center
1415 Ross Ave
El Centro, CA 92243
760-339-7267
760-482-5365(F)
www.ecrmc.org <http://www.ecrmc.org/>
Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments.
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Message: 9
Date: Wed, 19 May 2010 13:06:27 -0500
From: "Troutman, Kenneth A" <kenneth.a.troutman <@t> Vanderbilt.Edu>
Subject: [Histonet] IHC Validation on new instrument
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7B310892042DA74CB3590053F424CFE60B83F97011 <@t> ITS-HCWNEM06.ds.Vanderbilt.edu>
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Hi Laurie,
I have a Benchmark Ultra and a Benchmark XT from Ventana and they follow the basic steps, similar protocols and I needed to revalidate everything. They are sufficiently different (in my experience) that it warranted a complete revalidation. A part of the reasoning for this was some of the bulk reagents were different (a consideration that may lead you to not completely revalidate). For me, there were a few protocols that were quite different from one to the other. I would go to the trouble up front, that way there is no question later.
Good luck!
Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN 37232
615-343-9134
Message: 4
Date: Wed, 19 May 2010 07:59:30 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: [Histonet] IHC Validation on new instrument
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<57BE698966D5C54EAE8612E8941D768308BCAF81 <@t> EXCHANGE3.huntingtonhospital.com>
Content-Type: text/plain; charset="us-ascii"
What do others do when validating a new model of a piece of equipment - same manufacturer, same basic staining process, but an updated version of the equipment?
I've been told the protocols should be the same and that we only need to run three controls with three different but similar protocols to determine what looks best. Do you all think that is thorough enough, or would you run actual patient cases and compare old and new equipment? I don't see where the CAP checklist refers to new equipment - just new antibodies and new antibody lots.
Laurie Colbert
------------------------------
Message: 10
Date: Wed, 19 May 2010 14:20:42 -0400
From: Cindy DeRiso <cindy.deriso <@t> yale.edu>
Subject: [Histonet] PPE
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <4BF42BFA.6030006 <@t> yale.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Does any one use or require gloves when cutting and embedding? How about safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology
------------------------------
Message: 11
Date: Wed, 19 May 2010 11:37:30 -0700
From: sgoebel <@t> xbiotech.com
Subject: RE: [Histonet] Freezing spray artifact
To: "Martin,Erin" <Erin.Martin <@t> ucsf.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<20100519113730.9e2d9aa830e8449a2412eb1e4f2f067e.24d0cf7c87.wbe <@t> email04.secureserver.net>
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[DEL: 3D"" :DEL]
Picture on the left has the artifact, picture on the right is good
to go. Hope this
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
< XBiotech USA Inc.
8201 Eas (512)386-51
-------- Original Message --------
Subject: [Histonet] Freezing spray artifact
From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Date: Wed, May 19, 2010 9:09 am
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Has anyone run into a problem or artifact from freezing spray? I think
we descriptio Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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[1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
------------------------------
Message: 12
Date: Wed, 19 May 2010 13:38:43 -0500
From: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Subject: RE: [Histonet] Re: Grossing Technician Qualifications
To: 'Daniel Schneider' <dlschneider <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A1B6 <@t> EXCHMBC2.ad.ah.local>
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Things have changed. All "grossing" is back to being grossing.
Jan Mahoney
Omaha, NE
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
Sent: Wednesday, May 19, 2010 12:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Grossing Technician Qualifications
Just to clarify or perhaps cloud the picture a little more
Not all grossing is "grossing."
So when we're talking about transferring small biopsies, in their
entirety, from a formalin container to a cassette, and describing the size,
number, and color of the tissue pieces submitted, with no dissection or
knowledge of anatomy required, that's not "grossing" in the strict sense, at
least according to CAP the last time I looked, which admittedly was a couple
of years ago.
So what sort of educational achievements, training, or credentials are
required for the above?
Dan Schneider
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Message: 13
Date: Wed, 19 May 2010 13:15:08 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] PPE
To: <cindy.deriso <@t> yale.edu>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B10113B <@t> server.PremierLab.local>
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I require gloves when sectioning only because it helps eliminate
epithelial floaters, with respects to embedding the tech can choose to
or not to use gloves. No safety goggles are required.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cindy
DeRiso
Sent: Wednesday, May 19, 2010 12:21 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PPE
Does any one use or require gloves when cutting and embedding? How about
safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 19 May 2010 15:16:44 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] PPE
To: <cindy.deriso <@t> yale.edu>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9703E242FF <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"
We wear gloves when handling tissue and embedding. No gloves for sectioning and
no safety goggles.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cindy DeRiso
Sent: Wednesday, May 19, 2010 2:21 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PPE
Does any one use or require gloves when cutting and embedding? How about
safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology
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Message: 15
Date: Wed, 19 May 2010 14:18:46 -0500
From: Daniel Schneider <dlschneider <@t> gmail.com>
Subject: Re: [Histonet] Re: Grossing Technician Qualifications
To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AANLkTiltIGXW_P1pHbPu4FzATAgFWLl_VeHpYpcYHyiU <@t> mail.gmail.com>
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Really. Are you sure?
"Received in formalin, labeled with the patient's name and number, the
specimen consists of a single tan-gray fragment of tissue measuring 3
millimeters in greatest dimension, submitted entirely in A1" requires 60
some odd hours?
I have no gripe with requiring a certain degree of credentials for any
grossing which requires dissection, judgement, and knowledge of anatomy, but
this is merely counting, measuring, and transferring. I think the CAP term
was "processing" as distinguished from "grossing." So "processing" (in the
above sense, not in the histo sense) is gone now?
Is this coming from CAP? Could someone give me chapter and verse so that I
can make copies and discuss it with my colleagues locally?
Thanks,
On Wed, May 19, 2010 at 1:38 PM, Mahoney,Janice A <
Janice.Mahoney <@t> alegent.org> wrote:
> Things have changed. All "grossing" is back to being grossing.
> Jan Mahoney
> Omaha, NE
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
> Sent: Wednesday, May 19, 2010 12:58 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Re: Grossing Technician Qualifications
>
> Just to clarify or perhaps cloud the picture a little more
>
> Not all grossing is "grossing."
>
> So when we're talking about transferring small biopsies, in their
> entirety, from a formalin container to a cassette, and describing the size,
> number, and color of the tissue pieces submitted, with no dissection or
> knowledge of anatomy required, that's not "grossing" in the strict sense,
> at
> least according to CAP the last time I looked, which admittedly was a
> couple
> of years ago.
>
> So what sort of educational achievements, training, or credentials are
> required for the above?
>
> Dan Schneider
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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------------------------------
Message: 16
Date: Wed, 19 May 2010 13:00:39 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: [Histonet] IHC Validation (again)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<57BE698966D5C54EAE8612E8941D768308BCB03A <@t> EXCHANGE3.huntingtonhospital.com>
Content-Type: text/plain; charset="us-ascii"
Can anyone tell me if there is a specific question on the CAP checklist
that addresses revalidation of antibodies when starting up a new IHC
stainer?
Laurie Colbert
------------------------------
Message: 17
Date: Wed, 19 May 2010 13:10:36 -0700 (PDT)
From: Debbie Dreesen <ddreesen <@t> sbcglobal.net>
Subject: Re: [Histonet] alternative for bunsen burner?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <481061.10086.qm <@t> web81003.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Brandi,
The electric three well forceps warmer that Jackie mentioned is really handy and you can find it at most medical supply companies. They sell the exact same unit but for widely different prices. It's in the Cardinal catalogue for $600 Cat. No. M7323, Mfr. No. FW-120. Mercedes Medical sells it for $700 TRX FW120 and American MasterTech sells their's for $625 EQFW-120.
Debbie Dreesen, HT(ASCP)
Message: 2
Date: Tue, 18 May 2010 11:30:11 -0400
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: [Histonet] alternative for bunsen burner?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTil97WigWL23eyeryT15gxZBULVktNBJjAUYUn9Q <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello all,
Our hospital is moving our histology department across the hall and have
asked us if we can find an alternative to the bunsen burner so they don't
have to install a gas line. The pathologist has told me that there is some
machine that can be used instead of a flame to burn the forceps while
embedding (which is our main use for the bunsen flame). This is the only
lab I have ever worked at and I don't know what such a machine would be
called. If anyone knows the name, or any other alternative method can you
let me know. Model numbers/companies would be a bonus too! Thanks so much
for your help.
Brandi Higgins, BS, HT(ASCP)
------------------------------
Message: 18
Date: Wed, 19 May 2010 16:26:52 -0400
From: thomas.crowell <@t> novartis.com
Subject: [Histonet] Thomas Crowell is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF58DF6E49.73B4F2E7-ON85257728.007052F0-85257728.007052F2 <@t> ah.novartis.com>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office starting 05/19/2010 and will not return until
05/25/2010.
Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
------------------------------
Message: 19
Date: Wed, 19 May 2010 14:41:36 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] IHC Validation (again)
To: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B101142 <@t> server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
Laurie
I'm not aware of a particular question, but I would believe you would
have to perform some validation steps for each antibody. I would
approach it the same way you approach validating new lots of antisera.
The CAP paper on standardization of IHC recommends 25 different samples
when you initially validate an antibody - 10 samples that have high
levels of target antigen, 10 intermediate to low levels and 5 negative.
To revalidate new lots they recommend only 3 tissue samples - 1 high, 1
med to low, and 1 negative. Granted this only applies to routine
markers. For prognostic markers such as ER/PR and Her2 then additional
samples need to be tested - there are new guidelines for ER/ER out and
the new recommendations for validation for ER/ER are briefly reviewed in
the CAP Today April issue. Guidelines for validation of ER/PR will be
published in the June issue of Archives of Pathology and Laboratory
Medicine.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Wednesday, May 19, 2010 2:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation (again)
Can anyone tell me if there is a specific question on the CAP checklist
that addresses revalidation of antibodies when starting up a new IHC
stainer?
Laurie Colbert
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Wed, 19 May 2010 17:04:46 -0400
From: James L Burchette <burch007 <@t> mc.duke.edu>
Subject: Re: [Histonet] Freezing spray artifact
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFEDC715CE.3E65F3F5-ON85257728.0072FF6B-85257728.0073CB15 <@t> notes.duke.edu>
Content-Type: text/plain; charset="US-ASCII"
Erin -
Try this trick when using the freezing spray: take a folded kim wipe
tissue and fold it again, then again then the long fold is folded twice so
you end up with roughly a 1 inch by 1 inch multi layer slide wiper. Run
your finger down each crease and after the final fold, give it a little
pinch and twist so it holds its shape. You are probably wondering what
does this have to do with freezing spray artifact... Dip the square of Kim
Wipe in your water bath and press it on the cut surface of your tissue
block. It will act as an insulator when you spray the block surface. They
are also good for soaking the face of a block and are better than a wad of
tissue when wiping slides.
JB
"Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/19/2010 12:41 PM
To
histonet <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Freezing spray artifact
Has anyone run into a problem or artifact from freezing spray? I think we
may be having a problem with it but I can't find any pictures or
descriptions of what it looks like.
Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Wed, 19 May 2010 14:54:07 -0700
From: sgoebel <@t> xbiotech.com
Subject: [Histonet] Equipment Repair Dude
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<20100519145407.9e2d9aa830e8449a2412eb1e4f2f067e.7f63da1337.wbe <@t> email04.secureserver.net>
Content-Type: text/plain; charset="utf-8"
Hey ya'll,
I'm in the Austin Texas area and am in need fix a cryostat and a microtome (or at least com dia problem). Does anyone out there know of someone who could c and work on some of my equipment that won't charge the cost of just replacing it?
Thanks!!
Sarah Goebel,
Histotechnician
XBiotech USA Inc.
<
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
<
(512)386-5107
------------------------------
Message: 22
Date: Wed, 19 May 2010 18:08:03 -0400
From: "Andrew Burgeson" <napoli <@t> siscom.net>
Subject: [Histonet]
AANLkTik8dnsQt16RPMSS8_x5fgqQE_18-ctUeNp71V0L <@t> mail.gmail.com
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <4bf46143.268.24d1.1221708583 <@t> siscom.net>
Content-Type: text/plain; charset="iso-8859-1"
"One thing i forgot to mention wasthat when you embed, try
to orientate the
tissue so that the long axis (if there is one) lies in the
same direction as
the cutting stroke. when embedding, orientate the tissue at
a slight
diagonal, so that the knife dous not continously pass
through the tissue on
the cutting stroke -
(this works well for skins also, except make sure the
dermis is away from the knife)
I do not agree with the above statement about the "dermis
being embedded so as to be facing away from the blade." The
last tissue to hit the knife edge should be EPIDERMIS.
Dermis and SubQ fat should be the first tissues to hit the
blade. Perhaps this is what you meant by "dermis?"
Otherwise, I would agree with that methodology of
orientation and angle.
MethylMethacrylate bone embedding works very well from what
I understand. See link:
http://www.jhc.org/cgi/content/full/45/2/307
------------------------------
Message: 23
Date: Wed, 19 May 2010 17:09:29 -0500
From: "Rebecca Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Histonet
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E56C9F853A1F4725A3EFC73F1DCF8A39 <@t> CHURCH>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Does anyone have Peggy Wenk email or phone.
Thanks
------------------------------
Message: 24
Date: Wed, 19 May 2010 18:20:47 -0400
From: "Andrew Burgeson" <napoli <@t> siscom.net>
Subject: [Histonet] bone processing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4bf4643f.238.2918.1412147363 <@t> siscom.net>
Content-Type: text/plain; charset="iso-8859-1"
I have seen a lot of traffic on the site regarding bone
processing...If anyone is interested, I have personally
witnessed the following system being used very successfully
for the embedding and sectioning of bone, including bone
that has been surgically implanted with metal devices.
I just thought I would give this company's product(s) a
"plug" because I know that the "EXAKT System" works
extremely well for certain applications.
Anyone interested click the link below: (worth a look)
http://www.exaktusa.com/applications/
AB
------------------------------
Message: 25
Date: Wed, 19 May 2010 21:00:55 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Grossing Technician Qualifications
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTinji0wHETknKOY9c7m1C_MvGzsFVrcaXgVQD6hc <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Thanks to all who've been most helpful.
I probably shouldn't be admitting it, but I don't think I had enough
college science courses to be allowed to gross today. Maybe if they
don't find out that two of my biology courses were in paleontology. I
can gross a trilobite like you wouldn't believe!
Bob Richmond
Samurai Pathologist
Konxville TN
------------------------------
Message: 26
Date: Wed, 19 May 2010 21:55:37 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: RE: [Histonet] Freezing spray artifact
To: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu, "Martin, Erin"
<Erin.Martin <@t> ucsf.edu>
Message-ID:
<OF90B79D8E.1081D6E0-ON88257729.001B05DC-88257729.001AF4F9 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
We also had the appearance of burnt tissue when someone was using the
spray excessively.
"Sebree Linda A" <LSebree <@t> uwhealth.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/19/2010 11:07 AM
To
"Martin, Erin" <Erin.Martin <@t> ucsf.edu>, "histonet"
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
RE: [Histonet] Freezing spray artifact
Erin,
One can certainly get cracking of the tissue and surrounding paraffin
from a too intense application of freezing spray. This is usually
grossly visible, at least in the paraffin.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, May 19, 2010 11:09 AM
To: histonet
Subject: [Histonet] Freezing spray artifact
Has anyone run into a problem or artifact from freezing spray? I think
we may be having a problem with it but I can't find any pictures or
descriptions of what it looks like.
Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 78, Issue 28
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