[Histonet] IHC Validation (again)

[Liz Chlipala]

Laurie

I'm not aware of a particular question, but I would believe you would
have to perform some validation steps for each antibody.  I would
approach it the same way you approach validating new lots of antisera.
The CAP paper on standardization of IHC recommends 25 different samples
when you initially validate an antibody - 10 samples that have high
levels of target antigen, 10 intermediate to low levels and 5 negative.
To revalidate new lots they recommend only 3 tissue samples - 1 high, 1
med to low, and 1 negative.  Granted this only applies to routine
markers.  For prognostic markers such as ER/PR and Her2 then additional
samples need to be tested - there are new guidelines for ER/ER out and
the new recommendations for validation for ER/ER are briefly reviewed in
the CAP Today April issue.  Guidelines for validation of ER/PR will be
published in the June issue of Archives of Pathology and Laboratory
Medicine.

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Wednesday, May 19, 2010 2:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation (again)

Can anyone tell me if there is a specific question on the CAP checklist
that addresses revalidation of antibodies when starting up a new IHC
stainer?

 

Laurie Colbert

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