[Histonet] Freezing spray artifact

[Sebree Linda A]

Erin,  
One can certainly get cracking of the tissue and surrounding paraffin
from a too intense application of freezing spray.  This is usually
grossly visible, at least in the paraffin. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, May 19, 2010 11:09 AM
To: histonet
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?  I think
we may be having a problem with it but I can't find any pictures or
descriptions of what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

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