[Histonet] fibrous bone tissue

[Vicki Kalscheur]

The thickness of tissue would work better if thinner, however, if you can infiltrate in paraffin overnight for an extra 12-18 hours it should embed and section just fine.  I face the block warm; soak on ice for a short time and then section. I find that taking the cuts in deeper, after the block warms up in the chuck ( brush with a damp gauze a few times) , gives me beautiful sections of fibrous area and bone. Perhaps section thicker.  The blade must be very sharp. A D profile steel blade can be used ( although I have had good results with dispo blades).  I have also adjusted my knife angle more straight up.  Vicki