[Histonet] large fibrous bone tissue

[Wimer, Helen]

Hi Reul,  It is possible that you are not processing your bone long enough in the tissue processor.  If you would like to discuss this possibility, please email me.  Helen

Helen F Wimer HT (ASCP)
Smithsonian Institution
Department of Vertebrate Zoology
Washington, DC
(301) 496-1391
Wimerh <@t> si.edu
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jack Ratliff [ratliffjack <@t> hotmail.com]
Sent: Wednesday, May 19, 2010 1:33 AM
To: louise renton; Reuel Cornelia
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] large fibrous bone tissue

Louise has very good advice here as related to paraffin processing of
this tissue. I may even add to soak the block a little more before
taking the final sections. However, have you ever thought of
processing into MMA resin? If you have these capabilities you may be
very pleased with the results and find the microtomy less problematic.
Let me know if or how I can be of assistance!

Jack

On May 15, 2010, at 4:30 AM, louise renton <louise.renton <@t> gmail.com>
wrote:

> I have found this helps.....
> 1. Embed the tissue in  a dep mould, as this provides more
> stability, then
> 2. Face the block
> 3.. leave in -20 deg freezer overnight
> 4. remove from freezer and cut sections
> 5. If you have multiple blocks to work with, leave them in the
> freezer until
> ready to cut
>
> regards
> On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org
> >wrote:
>
>> How do you process a fibrous bone tissue ( 7 mm thick).  We have use
>> Paraffin Type 9 from Richard allan Scientific to embed works well
>> with our
>> bone femur( 7 mm) when cutting but on fibrous bone it does not give
>> us a
>> good result in cutting the blocks. It is like cutting a uterus
>> tissue but a
>> little bit harder. Please give me your opinion on how to remedy
>> this kind of
>> tissue not mentioning double embedding method or plastic. Thank you.
>>
>> Reuel Cornelia
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
> No trees were killed in the sending of this message.
> However, many electrons were terribly inconvenienced.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet