[Histonet] Mounting Medium Issues

[Jack Ratliff]

Jim,

I must say that I have used Eukitt exclusively for over 13 years and  
the only problem I have had with Eukitt is when I do not use enough  
and especially when coverslipping thicker sections. It is xylenes  
based so maybe you are experiencing excessive evaporation during  
drying??? If you are diluting the Eukitt with xylenes so that it flows  
better (not as viscious) and reduces the chance for air bubbles, this  
might cause excessive shrinkage and/or the problems you are  
experiencing.

Jack

On May 14, 2010, at 3:22 PM, "Herrick, James L. (Jim)" <Herrick.James <@t> mayo.edu 
 > wrote:

> Hello everyone,
>
> Hope you have all had a good week!!
>
> Has anyone had any negative experiences using Eukitt as your mounting
> media? We embed our specimens (human - iliac crest, animal - femurs,
> tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
> of time that a fairly large number of our slides are beginning to show
> signs of the medium breaking up below the cover slip. It looks as if
> there are large air pockets left behind. We are not yet sure why  
> this is
> happening. Would any of you geniuses have a resolution for us or  
> know of
> a better mounting media to use with plastic embedded specimens -
> brightfield and fluorescence? Thanks for your expertise. It is very  
> much
> appreciated.
>
> Have a great weekend!!
>
> Jim
>
>
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