FW: [Histonet] Mounting Medium Issues

[Herrick, James L. (Jim)]

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Herrick,
James L. (Jim)
Sent: Friday, May 14, 2010 3:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium Issues

Hello everyone,

Hope you have all had a good week!!

Has anyone had any negative experiences using Eukitt as your mounting
media? We embed our specimens (human - iliac crest, animal - femurs,
tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
of time that a fairly large number of our slides are beginning to show
signs of the medium breaking up below the cover slip. It looks as if
there are large air pockets left behind. We are not yet sure why this is
happening. Would any of you geniuses have a resolution for us or know of
a better mounting media to use with plastic embedded specimens -
brightfield and fluorescence? Thanks for your expertise. It is very much
appreciated.

Have a great weekend!!

Jim


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