[Histonet] Alpha Dystroglycan Immuno

[Malika Benatti]

** Proprietary **
** Reply Requested When Convenient **


Having issue with Millipore Alpha Dystroglycan antibody.

Millipore recommend 1:100 dilution so we  tried various titrations ranging from 1:100, 3:200 to 9:200, normal and abnormal muscle tissue with very poor results. After staining up to  9:200 dilution immuno come out patchy not strong enough

Any one on the histonet list use this antibody, if so at what dilution.

any input would be really appreciated

Malika



Malika Benatti
 
Specialist BMS 
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH

 
Tel:  +44 20 7405 9200 ext 5475
Fax:  +44 20 7829 7875
 
benatm <@t> gosh.nhs.uk


*********************************************************************************************************
This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it.

Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful.

Thank you for your co-operation.

*********************************************************************************************************