SPAM-LOW: Re: [Histonet] Prolonged FFPE slide and block storage

[Patsy Ruegg]

To store cut sections for use later for IHC I do not bake them to melt the
paraffin, they keep better, melt them just before use.  I also store the cut
slides at 4dc.  For really precious control blocks, I reseal them with
paraffin (just dip in hot paraffin and then cool) and I do also store the
blocks as 4dc.  We just ran into a problem with tonsil sections going bad
for IMP3 ab., this is a real concern.

Best regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joseph Saby
Sent: Friday, May 14, 2010 4:21 PM
To: Perry, Samuel; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] Prolonged FFPE slide and block storage

Sam-

Are you storing sectioned slides?

What we do is to deparaffinize and coverslip sectioned but unstained
slides.  I haven' had to go back, but I believe they will store that way a
long time.

Joe Saby, BA HT




________________________________
From: "Perry, Samuel" <Samuel_Perry <@t> DFCI.HARVARD.EDU>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Fri, May 14, 2010 4:16:06 PM
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose
their
antigenicity, since we use them for IHC.  

Any suggestions for inexpensive methods for prolonged storage of FFPE slides
and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


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